甲胎蛋白单克隆免疫组化荧光抗体(检测)

中文名称甲胎蛋白单克隆抗体(检测)
别    名Alpha Fetoprotein; Alpha-fetoprotein; AFP; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.  
文献应用：

规格价格50ul  100ul  
研究领域肿瘤  
抗体来源Mouse
克隆类型Monoclonal
克 隆 号A4
交叉反应 Human, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量65kDa
细胞定位分泌型蛋白 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原AFP protein:full length 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M PBS(pH7.4) with 0.03% Proclin300.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
甲胎蛋白单克隆抗体(检测)产品介绍background:
Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. 

Function:
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Subunit:
Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma. Synthesized by the fetal liver and yolk sac.

Similarity:
Belongs to the ALB/AFP/VDB family. 
Contains 3 albumin domains.

SWISS:
P02771

Gene ID:
174

Database links:
Entrez Gene: 174 Human
Omim: 104150 Human
SwissProt: P02771 Human
Unigene: 518808 Human


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

甲胎蛋白单克隆抗体(检测)AFP是由胚胎卵黄囊细胞，胚胎肝细胞和胎儿肠道细胞合成的一种糖蛋白。主要用于原发性或转移性肝细胞癌、肝样胃癌和性腺或性腺外某些生殖细胞肿瘤(如内胚窦瘤)的诊断和鉴别诊断。
AFP敏感度较低，特异性强，大约60%左右的肝细胞肝癌阳性，在低分化肿瘤中常呈阳性表达。
该抗体与bsm-1621M（包被）（Anti-Alpha Fetoprotein）配对使用；主要用于ELISA试剂盒，胶体金试剂条制备生产都很好。大量订购可来电商议。

产品图片	Sample:  Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-AFP(A4) (bsm-1622M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD