细胞色素C抗体

英文名称Cytochrome C
中文名称细胞色素C抗体
别    名CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome c; MSA06; THC4.  
规格50ul  100ul  200ul

研究领域肿瘤  心血管  细胞生物  神经生物学  信号转导  细胞凋亡  脂蛋白  线粒体  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Guinea Pig, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量12.8/26kDa
细胞定位细胞浆 细胞膜 线粒体
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Cytochrome C:51-105/105 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis. 


Function:
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Subcellular Location:
Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.

Post-translational modifications:
Binds 1 heme group per subunit. 
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

DISEASE:
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

Similarity:
Belongs to the cytochrome c family.

SWISS:
P99999

Gene ID:
54205

Database links:
Entrez Gene: 54205 Human
Entrez Gene: 13063 Mouse
Entrez Gene: 25309 Rat
Omim: 123970 Human
SwissProt: P99999 Human
SwissProt: P62897 Mouse
SwissProt: P62898 Rat
Unigene: 437060 Human
UniProtKB/Swiss-Prot: P99999.2
 


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

细胞色素C（cytC）是一种电子传递链蛋白为线粒体呼吸链必须的成份之一。在哺乳动物细胞中，如此高度保守性蛋白常分布在线立体内膜。
新近研究证明细胞浆中细胞色素C为激活细胞调亡所必需的因子。在调亡的过程中，细胞色素C从线立体膜被易位到细胞浆，由细胞色素C激活Caspase-3（CPP32）。
细胞色素C的易位可被过量表达的Bcl-2阻断。细胞色素B与细胞色素C1和Rieske蛋白相结合而形成复合物III（也称细胞色素B-C1复合物）参与细胞呼吸链。该蛋白动物种属间同源性较高；如 ：猪、犬、牛、鸡、豚鼠等。

产品图片

Sample:  Heart(Rat)A549 Cell Lysate at 40 ug Primary: Anti-Cytochrome C (bs-0013R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/26 kD Observed band size: 26 kD Sample: Cerebellum (Mouse) lysate at 30ug; Primary: Anti-Cytochrome C (bs-0013R) at 1:300;  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size:12.8/26 kD Observed band size:27 kD Sample: Brain (Mouse) lysate at 30ug; Primary: Anti-Cytochrome C (bs-0013R) at 1:300;  Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;  Predicted band size:12.8/26 kD Observed band size:28 kD Sample: Hela Cell lysate at 30ug; Primary: Anti-Cytochrome C (bs-0013R) at 1:300;  Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;  Predicted band size:12.8/26 kD Observed band size:26 kD Sample:  heart (Mouse) Lysate at 40 ug Primary: Anti-Cytochrome C (bs-0013R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/28 kD Observed band size: 13/28 kD Blank control: HepG2(blue).  Primary Antibody:Rabbit Anti-Cytochrome C antibody (bs-0013R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;  Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0013R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.