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- 详细信息
- 技术资料
- 抗体名:
磷酸化Rho相关蛋白激酶2抗体价格
- 抗体英文名:
Anti-phospho-ROCK2(Thr249)
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海一研
- 库存:
55
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated Synthesised phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249
- 规格:
0.1ml/100μg
英文名称 Anti-phospho-ROCK2(Thr249)
中文名称 磷酸化Rho相关蛋白激酶2抗体价格
别 名 ROCK2(phospho T249); p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig
产品类型 一抗 磷酸化抗体
研究领域 细胞生物 免疫学 信号转导 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 153kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Function : Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus.
Subunit : Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1.
Subcellular Location : Cytoplasm. Cell membrane; Peripheral membrane protein (By similarity). Nucleus. Cytoplasm, cytoskeleton, centrosome.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.
Database links : UniProtKB/Swiss-Prot: O75116.4
ROCK2(Rho kinase alpha)是ROCK1的同工酶。是一个丝/苏氨基酸激酶,可调节细胞因子,平滑肌收缩和肌动蛋白的形成。
磷酸化Rho相关蛋白激酶2抗体价格的功能
抗体的主要功能是与抗原(包括外来的和自身的)相结合,从而有效地清除侵入机体内的微生物、寄生虫等异物,抗体(antibody)是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活,也可能无效但有时也会对机体造成病理性损害,如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生,对人体可造成危害。
公司抗体应用于WB、IHC、IF、ELISA等实验中,磷酸化Rho相关蛋白激酶2抗体价格品质保证,提供完善的售前、售中、售后服务!欢迎来电咨询或联系在线客服!我们将竭诚为您服务!
磷酸化Rho相关蛋白激酶2抗体价格的优点:
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子,稳定性好,室温下可以长期保存;
②制备简单,操作方便,可以进行批量;
③不必免疫动物,且可获得免疫动物所不能得到的“抗体”;
④可以反复使用;
⑤价格低廉。因此,设计、合成既具有类似生物抗体的高亲合性和高专一性,又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体,在部分领域替代生物抗体以进行仿生分子识别,或者完成一些生物抗体所不能完成的工作,具有重要的科学意义,在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗,品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌,品种多达7000多种,由于产品的数量众多,产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
磷酸化Rho相关蛋白激酶2抗体价格具有高度的特异性,免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来,以此作为抗原或半抗原,通过免疫动物后获得特异性的抗体,再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的,因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来,以其达到对组织或细胞中的未知抗原进行定性,定位或定量的研究。
磷酸化Rho相关蛋白激酶2抗体价格包括以下几个步骤:
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试,看看是否成功免疫。
5、如果成功免疫,杀死实验动物,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
Columbianadin 二氢欧山芹醇当归酸酯 5058-13-9 20mg
Neoagarohexaose 新琼六糖 25023-93-2 10mg
Abscisic acid 脱落酸 21293-29-8 20mg
L-Cysteine L-半胱氨酸 52-90-4 200mg
0.2ml八连排PCR管(平盖),10盒/箱 125排 盒
Hydroxyurea 羟基脲 1g 瓶
Curcumin 姜黄素 5g 瓶
Schizandrin B 五味子乙素 61281-37-6 20mg
Schisantherin A 五味子酯甲 58546-56-8 20mgphospho-MAP4K4(Ser631) 磷酸化丝裂原活化蛋白激酶MAP4K4抗体规格: 0.1mlphospho-DDX58 (Thr170) 磷酸化DDX58抗体规格: 0.1ml
Proteasome 19S S3 蛋白酶体PSMD3抗体规格: 0.2mlEAAT2 胶质细胞谷氨酸运载蛋白2抗体规格: 0.1ml
磷酸化Rho相关蛋白激酶2抗体价格Mouse Anti-Goat IgG/APC APC标记的小鼠抗羊IgG规格: 0.1mlRabbit Anti-human IgM/PE-CY5 PE-CY5标记的兔抗人IgM规格: 0.1ml
phospho-Androgen Receptor(Ser660) 磷酸化雄激素受体抗体规格: 0.1mlNFAT4 核因子活化T细胞胞浆蛋白3抗体 0.2ml
Donkey Anti-rabbit IgG/Alexa Fluor 350 Alexa Fluor 350标记的驴抗兔IgG规格: 0.1mlFactor VIII 第八因子抗体 0.1ml
GLO1 乙二醛酶1抗体规格: 0.1mlTIMP-1(CT) 金属蛋白酶组织抑制因子-1抗体(C端) 0.1ml
ARRDC4 抑制蛋白结构域蛋白4抗体规格: 0.2mlADK/AK 苷酸激酶抗体 0.1ml
FGF1/AFGF 酸性成纤维细胞生因子抗体规格: 0.1mlCG6856/CG6856-PA 果蝇CG6856-PA抗体规格: 0.5ml
ARSH 芳香基硫酸酯酶H抗体规格: 0.2mlPhospho-CHK2 (ser33) 磷酸化细胞周期检测点激酶2抗体规格: 0.1ml
Goat Anti-Bovine IgG/PE PE标记的羊抗牛IgG规格: 0.1mlCIDEB 促进凋亡基因抗体规格: 0.2ml
中文名称 磷酸化Rho相关蛋白激酶2抗体价格
别 名 ROCK2(phospho T249); p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig
产品类型 一抗 磷酸化抗体
研究领域 细胞生物 免疫学 信号转导 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 153kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Function : Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus.
Subunit : Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1.
Subcellular Location : Cytoplasm. Cell membrane; Peripheral membrane protein (By similarity). Nucleus. Cytoplasm, cytoskeleton, centrosome.
Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.
Database links : UniProtKB/Swiss-Prot: O75116.4
ROCK2(Rho kinase alpha)是ROCK1的同工酶。是一个丝/苏氨基酸激酶,可调节细胞因子,平滑肌收缩和肌动蛋白的形成。
磷酸化Rho相关蛋白激酶2抗体价格的功能
抗体的主要功能是与抗原(包括外来的和自身的)相结合,从而有效地清除侵入机体内的微生物、寄生虫等异物,抗体(antibody)是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活,也可能无效但有时也会对机体造成病理性损害,如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生,对人体可造成危害。
公司抗体应用于WB、IHC、IF、ELISA等实验中,磷酸化Rho相关蛋白激酶2抗体价格品质保证,提供完善的售前、售中、售后服务!欢迎来电咨询或联系在线客服!我们将竭诚为您服务!
磷酸化Rho相关蛋白激酶2抗体价格的优点:
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子,稳定性好,室温下可以长期保存;
②制备简单,操作方便,可以进行批量;
③不必免疫动物,且可获得免疫动物所不能得到的“抗体”;
④可以反复使用;
⑤价格低廉。因此,设计、合成既具有类似生物抗体的高亲合性和高专一性,又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体,在部分领域替代生物抗体以进行仿生分子识别,或者完成一些生物抗体所不能完成的工作,具有重要的科学意义,在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗,品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌,品种多达7000多种,由于产品的数量众多,产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
磷酸化Rho相关蛋白激酶2抗体价格具有高度的特异性,免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来,以此作为抗原或半抗原,通过免疫动物后获得特异性的抗体,再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的,因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来,以其达到对组织或细胞中的未知抗原进行定性,定位或定量的研究。
磷酸化Rho相关蛋白激酶2抗体价格包括以下几个步骤:
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试,看看是否成功免疫。
5、如果成功免疫,杀死实验动物,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
Columbianadin 二氢欧山芹醇当归酸酯 5058-13-9 20mg
Neoagarohexaose 新琼六糖 25023-93-2 10mg
Abscisic acid 脱落酸 21293-29-8 20mg
L-Cysteine L-半胱氨酸 52-90-4 200mg
0.2ml八连排PCR管(平盖),10盒/箱 125排 盒
Hydroxyurea 羟基脲 1g 瓶
Curcumin 姜黄素 5g 瓶
Schizandrin B 五味子乙素 61281-37-6 20mg
Schisantherin A 五味子酯甲 58546-56-8 20mgphospho-MAP4K4(Ser631) 磷酸化丝裂原活化蛋白激酶MAP4K4抗体规格: 0.1mlphospho-DDX58 (Thr170) 磷酸化DDX58抗体规格: 0.1ml
Proteasome 19S S3 蛋白酶体PSMD3抗体规格: 0.2mlEAAT2 胶质细胞谷氨酸运载蛋白2抗体规格: 0.1ml
磷酸化Rho相关蛋白激酶2抗体价格Mouse Anti-Goat IgG/APC APC标记的小鼠抗羊IgG规格: 0.1mlRabbit Anti-human IgM/PE-CY5 PE-CY5标记的兔抗人IgM规格: 0.1ml
phospho-Androgen Receptor(Ser660) 磷酸化雄激素受体抗体规格: 0.1mlNFAT4 核因子活化T细胞胞浆蛋白3抗体 0.2ml
Donkey Anti-rabbit IgG/Alexa Fluor 350 Alexa Fluor 350标记的驴抗兔IgG规格: 0.1mlFactor VIII 第八因子抗体 0.1ml
GLO1 乙二醛酶1抗体规格: 0.1mlTIMP-1(CT) 金属蛋白酶组织抑制因子-1抗体(C端) 0.1ml
ARRDC4 抑制蛋白结构域蛋白4抗体规格: 0.2mlADK/AK 苷酸激酶抗体 0.1ml
FGF1/AFGF 酸性成纤维细胞生因子抗体规格: 0.1mlCG6856/CG6856-PA 果蝇CG6856-PA抗体规格: 0.5ml
ARSH 芳香基硫酸酯酶H抗体规格: 0.2mlPhospho-CHK2 (ser33) 磷酸化细胞周期检测点激酶2抗体规格: 0.1ml
Goat Anti-Bovine IgG/PE PE标记的羊抗牛IgG规格: 0.1mlCIDEB 促进凋亡基因抗体规格: 0.2ml
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磷酸化Rho相关蛋白激酶2抗体价格
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