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- 详细信息
- 技术资料
- 抗体名:
磷酸化细胞周期检查控制蛋白质抗体
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海一研
- 库存:
34
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
ed phosphopeptide derived from human RAD9 around the phosphorylation site of Tyr28 [EL(p-Y)LE]
- 规格:
0.1ml/100μg
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英文名称 Anti-phospho-RAD9(Tyr28)
中文名称 磷酸化细胞周期检查控制蛋白质抗体说明书
别 名 RAD9A(phospho Y28); Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9A_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 免疫学 染色质和核信号 信号转导 细胞凋亡 细胞周期蛋白 转录调节因子
蛋白分子量 predicted molecular weight: 43kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RAD9 around the phosphorylation site of Tyr28 [EL(p-Y)LE]
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq].
Function : Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Subunit : Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.
Subcellular Location : Nucleus.
Post-translational modifications : Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Similarity : Belongs to the rad9 family.
Rad9由391个AA构成,Rad9有很多重要生物学功能,如:可调控DNA损伤反应、调节细胞的周期、对损伤的DNA进行修复、诱导细胞凋亡、调控部分基因的转录、具有3’端到5’端核酸外切酵素(exonuclease)的活性、促进核糖核酸的生合成以及参与胚胎发育过程。曾有报道指出Rad9在调节细胞周期、诱导细胞凋亡及维持整个基因体的完整性等方面的生物功能都和p53极为相似,因此Rad9及p53这两种蛋白在功能上可能具有相互协调作用。
磷酸化细胞周期检查控制蛋白质抗体说明书储存温度:
存在于血清、组织培养上清液或腹水中的抗体在-20℃下能够长期储存。低温存放不会损伤抗体活性。抗体的工作液通常储存于4℃中。此温度下,抗体的活性能稳定数月至数年亦不损失。抗体溶液不应反复冻融,因为反复冻融将导致部分抗体失活并产生不需要的蛋白—蛋白凝聚物。抗体的凝聚能导致活性丧失。这是由于抗原结合位点的空间构象被破坏,或由于产生的不溶物被离心或过滤而除去。冷凝蛋白类的抗体不能存放于4℃。因为在4℃下此类抗体会发生沉淀。一些鼠源性IgG3亚类的抗体有此特性。如果抗体在4℃下发生沉淀,就应该存放于室温下的叠氮钠溶液中。
磷酸化细胞周期检查控制蛋白质抗体说明书的分类:
按作用对象,可将其分为抗毒素、抗菌抗体、抗病毒抗体和亲细胞抗体(能与细胞结合的免疫球蛋白,如1型变态反应中的lgE反应素抗体,能吸附在靶细胞膜上);按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类;按抗体的来源,可 将其分为天然抗体和免疫抗体。抗体就是免疫球蛋白,是改变了的球蛋白分子。由特异性抗原刺激产生,抗体的产生是由于抗原侵入人体后引起各种免疫细胞相互作用,使淋巴细胞中的B细胞分化增殖而形成浆细胞,浆细胞可产生分泌抗体。
钙离子荧光探针Rhod-2, AM 超级 大包装 Rhod-2, AM UltraPure Grade Bulk packaging
6-荧光素氨基磷酯 6-Fluorescein phosphoramidite
iFluor 514-标记链霉亲和素偶联物 1 mg/ml iFluor 514-streptavidin conjugate 1 mg/ml
5-FAM炔烃 5-FAM Alkyne
6-HEX 叠氮化物 6-HEX azide
2C-D (hydrochloride) (50 mg) 2,5-dimethoxy-4-methyl-benzeneethanamine, monohydrochloride; 2,5-Dimethoxy-4-methylphenethylamine; 2C-D (hydrochloride)
BIM5078 (10 mg) 1-[(2-chloro-5-nitrophenyl)sulfonyl]-2-methyl-1H-benzimidazole; BIM5078
17-phenyl trinor Prostaglandin D2 (100 mg) 9。alpha。,15S-dihydroxy-11-oxo-17-phenyl-18,19,20-trinor-prosta-5Z,13E-dien-1-oic acid; 17-phenyl trinor PGD2; 17-phenyl trinor Prostaglandin D2
2-amino-5-methyl-4-phenyl Thiazole (250 mg) 5-methyl-4-phenyl-2-thiazolamine; NSC 54435; 2-amino-5-methyl-4-phenyl Thiazolephgophs-ATG4C(Ser398) 磷酸化自噬相关蛋白4C抗体规格: 0.1mlDRIP1/C14orf28 多巴胺受体相互作用蛋白1抗体规格: 0.2ml
磷酸化细胞周期检查控制蛋白质抗体说明书phospho-Rab24(Tyr17) 磷酸化ras基因相关蛋白Rab24抗体规格: 0.1mlRabbit Anti-dog IgG/APC APC标记的兔抗狗IgG规格: 0.1ml
phospho-RUNX1(Ser266) 磷酸化急性髓细胞白病1蛋白抗体规格: 0.1mlER-Alpha 雌激素受体α抗体规格: 0.1ml
SARS putative orflab polyprotein 冠状病毒抗体规格: 0.2mlTIP30 TIP30蛋白抗体规格: 0.2ml
Phospho-Smad1/5(Ser463/465) 磷酸化细胞信号转导分子Smad-1/5抗体规格: 0.1mlrHPV 重组人瘤病毒抗体规格: 0.2ml
SMMHC/Myosin 平滑肌肌球蛋白重链抗体规格: 0.1mlGLT8D2 糖基转移酶GLT8D2抗体规格: 0.2ml
ARSF 芳香基硫酸酯酶F抗体规格: 0.2mlTIMP-3 金属蛋白酶组织抑制因子-3抗体规格: 0.1ml
FBXO38 F-box蛋白38抗体规格: 0.2mlCD3/CD3-ε CD3抗体规格: 0.1ml
ZNRF4 锌指/环指蛋白4抗体规格: 0.2mlV.cholera Ogawa 01群稻叶型霍乱弧菌抗体规格: 0.2ml
RNF138 环指蛋白138抗体规格: 0.2mlDNPK1 DNA依赖蛋白激酶催化亚基抗体规格: 0.1ml
磷酸化细胞周期检查控制蛋白质抗体说明书按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类。
IgM抗体是免疫应答中首先分泌的抗体。它们在与抗原结合后启动补体的级联反应。它们还把入侵者相互连接起来,聚成一堆便于巨噬细胞的吞噬;
IgG抗体激活补体,中和多种毒素。IgG持续的时间长,是 唯一能在母亲妊娠期穿过胎盘保护胎儿的抗体。他们还从乳腺分泌进入初乳,使新生儿得 到保护;
IgA抗体进入身体的黏 膜表面,包括呼吸、消化、生殖等管道的黏膜,中和感染因子。还可以通过母乳的初乳把这种抗体输送到新生儿的消化道黏膜中,是在母乳中含量最多,最为重要的一类抗体;
IgE抗体的尾部与嗜碱细胞、肥大细胞的细胞膜结合。当抗体与抗原结合后,嗜碱细胞与肥大细胞释放组织胺一类物质促进炎症的发展。这也是引发速发型过敏反应的抗体;
IgD抗体的作用还不太清楚。它们主要出现在成熟的B淋巴细胞表面上,可能与B细胞的分化有关。(IgD于1995年从人骨髓瘤蛋白中发现,分子量为175kD,主要由扁桃体、脾等处浆细胞产生,人血清中IgD浓度为3~40μg/ml,不到血清总Ig的1%,在个体发育中合成较晚。IgD铰链区很长,且对蛋白酶水解敏感,因此IgD半衰期很短,仅2.8天。血清中IgD确切的免疫功能尚不清楚。在B细胞分化到成熟B细胞阶段,除了表达SmIgD,抗原刺激后表现为免疫耐受。成熟B细胞活化后或者活化后或者变成记忆B细胞时,SmIgD逐渐消失。
英文名称 Anti-phospho-RAD9(Tyr28)
中文名称 磷酸化细胞周期检查控制蛋白质抗体说明书
别 名 RAD9A(phospho Y28); Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9A_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 免疫学 染色质和核信号 信号转导 细胞凋亡 细胞周期蛋白 转录调节因子
蛋白分子量 predicted molecular weight: 43kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RAD9 around the phosphorylation site of Tyr28 [EL(p-Y)LE]
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq].
Function : Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Subunit : Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.
Subcellular Location : Nucleus.
Post-translational modifications : Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Similarity : Belongs to the rad9 family.
Rad9由391个AA构成,Rad9有很多重要生物学功能,如:可调控DNA损伤反应、调节细胞的周期、对损伤的DNA进行修复、诱导细胞凋亡、调控部分基因的转录、具有3’端到5’端核酸外切酵素(exonuclease)的活性、促进核糖核酸的生合成以及参与胚胎发育过程。曾有报道指出Rad9在调节细胞周期、诱导细胞凋亡及维持整个基因体的完整性等方面的生物功能都和p53极为相似,因此Rad9及p53这两种蛋白在功能上可能具有相互协调作用。
磷酸化细胞周期检查控制蛋白质抗体说明书储存温度:
存在于血清、组织培养上清液或腹水中的抗体在-20℃下能够长期储存。低温存放不会损伤抗体活性。抗体的工作液通常储存于4℃中。此温度下,抗体的活性能稳定数月至数年亦不损失。抗体溶液不应反复冻融,因为反复冻融将导致部分抗体失活并产生不需要的蛋白—蛋白凝聚物。抗体的凝聚能导致活性丧失。这是由于抗原结合位点的空间构象被破坏,或由于产生的不溶物被离心或过滤而除去。冷凝蛋白类的抗体不能存放于4℃。因为在4℃下此类抗体会发生沉淀。一些鼠源性IgG3亚类的抗体有此特性。如果抗体在4℃下发生沉淀,就应该存放于室温下的叠氮钠溶液中。
磷酸化细胞周期检查控制蛋白质抗体说明书的分类:
按作用对象,可将其分为抗毒素、抗菌抗体、抗病毒抗体和亲细胞抗体(能与细胞结合的免疫球蛋白,如1型变态反应中的lgE反应素抗体,能吸附在靶细胞膜上);按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类;按抗体的来源,可 将其分为天然抗体和免疫抗体。抗体就是免疫球蛋白,是改变了的球蛋白分子。由特异性抗原刺激产生,抗体的产生是由于抗原侵入人体后引起各种免疫细胞相互作用,使淋巴细胞中的B细胞分化增殖而形成浆细胞,浆细胞可产生分泌抗体。
钙离子荧光探针Rhod-2, AM 超级 大包装 Rhod-2, AM UltraPure Grade Bulk packaging
6-荧光素氨基磷酯 6-Fluorescein phosphoramidite
iFluor 514-标记链霉亲和素偶联物 1 mg/ml iFluor 514-streptavidin conjugate 1 mg/ml
5-FAM炔烃 5-FAM Alkyne
6-HEX 叠氮化物 6-HEX azide
2C-D (hydrochloride) (50 mg) 2,5-dimethoxy-4-methyl-benzeneethanamine, monohydrochloride; 2,5-Dimethoxy-4-methylphenethylamine; 2C-D (hydrochloride)
BIM5078 (10 mg) 1-[(2-chloro-5-nitrophenyl)sulfonyl]-2-methyl-1H-benzimidazole; BIM5078
17-phenyl trinor Prostaglandin D2 (100 mg) 9。alpha。,15S-dihydroxy-11-oxo-17-phenyl-18,19,20-trinor-prosta-5Z,13E-dien-1-oic acid; 17-phenyl trinor PGD2; 17-phenyl trinor Prostaglandin D2
2-amino-5-methyl-4-phenyl Thiazole (250 mg) 5-methyl-4-phenyl-2-thiazolamine; NSC 54435; 2-amino-5-methyl-4-phenyl Thiazolephgophs-ATG4C(Ser398) 磷酸化自噬相关蛋白4C抗体规格: 0.1mlDRIP1/C14orf28 多巴胺受体相互作用蛋白1抗体规格: 0.2ml
磷酸化细胞周期检查控制蛋白质抗体说明书phospho-Rab24(Tyr17) 磷酸化ras基因相关蛋白Rab24抗体规格: 0.1mlRabbit Anti-dog IgG/APC APC标记的兔抗狗IgG规格: 0.1ml
phospho-RUNX1(Ser266) 磷酸化急性髓细胞白病1蛋白抗体规格: 0.1mlER-Alpha 雌激素受体α抗体规格: 0.1ml
SARS putative orflab polyprotein 冠状病毒抗体规格: 0.2mlTIP30 TIP30蛋白抗体规格: 0.2ml
Phospho-Smad1/5(Ser463/465) 磷酸化细胞信号转导分子Smad-1/5抗体规格: 0.1mlrHPV 重组人瘤病毒抗体规格: 0.2ml
SMMHC/Myosin 平滑肌肌球蛋白重链抗体规格: 0.1mlGLT8D2 糖基转移酶GLT8D2抗体规格: 0.2ml
ARSF 芳香基硫酸酯酶F抗体规格: 0.2mlTIMP-3 金属蛋白酶组织抑制因子-3抗体规格: 0.1ml
FBXO38 F-box蛋白38抗体规格: 0.2mlCD3/CD3-ε CD3抗体规格: 0.1ml
ZNRF4 锌指/环指蛋白4抗体规格: 0.2mlV.cholera Ogawa 01群稻叶型霍乱弧菌抗体规格: 0.2ml
RNF138 环指蛋白138抗体规格: 0.2mlDNPK1 DNA依赖蛋白激酶催化亚基抗体规格: 0.1ml
磷酸化细胞周期检查控制蛋白质抗体说明书按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类。
IgM抗体是免疫应答中首先分泌的抗体。它们在与抗原结合后启动补体的级联反应。它们还把入侵者相互连接起来,聚成一堆便于巨噬细胞的吞噬;
IgG抗体激活补体,中和多种毒素。IgG持续的时间长,是 唯一能在母亲妊娠期穿过胎盘保护胎儿的抗体。他们还从乳腺分泌进入初乳,使新生儿得 到保护;
IgA抗体进入身体的黏 膜表面,包括呼吸、消化、生殖等管道的黏膜,中和感染因子。还可以通过母乳的初乳把这种抗体输送到新生儿的消化道黏膜中,是在母乳中含量最多,最为重要的一类抗体;
IgE抗体的尾部与嗜碱细胞、肥大细胞的细胞膜结合。当抗体与抗原结合后,嗜碱细胞与肥大细胞释放组织胺一类物质促进炎症的发展。这也是引发速发型过敏反应的抗体;
IgD抗体的作用还不太清楚。它们主要出现在成熟的B淋巴细胞表面上,可能与B细胞的分化有关。(IgD于1995年从人骨髓瘤蛋白中发现,分子量为175kD,主要由扁桃体、脾等处浆细胞产生,人血清中IgD浓度为3~40μg/ml,不到血清总Ig的1%,在个体发育中合成较晚。IgD铰链区很长,且对蛋白酶水解敏感,因此IgD半衰期很短,仅2.8天。血清中IgD确切的免疫功能尚不清楚。在B细胞分化到成熟B细胞阶段,除了表达SmIgD,抗原刺激后表现为免疫耐受。成熟B细胞活化后或者活化后或者变成记忆B细胞时,SmIgD逐渐消失。
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磷酸化细胞周期检查控制蛋白质抗体说明书
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