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- 文献和实验
- 技术资料
- 抗体名:
RAN GTPase酶激活蛋白1抗体
- 抗体英文名:
Anti-RanGAP1
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
PBS, pH 7.4 产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海一研
- 库存:
47
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human RanGAP1
- 规格:
0.2ml/200μg
英文名称 Anti-RanGAP1
中文名称 RAN GTPase酶激活蛋白1抗体说明书
别 名 Fug 1; Fug-1; Fug1; KIAA1835; RAGP1_HUMAN; Ran 1; RAN GTPase activating protein 1; RAN GTPase-activating protein 1; Ran-1; Ran1; RANGAP 1; RanGAP1; RANGAP1; SD antibody Segregation distorter homolog; Segregation distortion.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig
产品类型 一抗
研究领域 细胞生物 免疫学 细胞周期蛋白 细胞分化 表观遗传学
蛋白分子量 predicted molecular weight: 65kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human RanGAP1
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq, Jul 2008].
Function : GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Subunit : Homodimer. Forms a tight complex in association with RANBP2/NUP358 and UBE2I/UBC9, the ubiquitin-conjugating enzyme E2. Interacts with UBE2I; the interaction conjugates SUMO1 to RANGAP1, and subsequently stabilizes interactions of sumoylated RANGAP1 with RANBP2/NUP358. The SUMO1/RANGAP1/UBC9/NUP358 complex associates with nuclear pore complexes. Interacts with TRAF6.
Subcellular Location : Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Note=Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
Tissue Specificity : Highly expressed in brain, thymus and testis.
Post-translational modifications : Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
Similarity : Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats.
Database links : UniProtKB/Swiss-Prot: P46060.1
英文名称 Anti-Retinoic acid induced 17
中文名称 维甲酸诱导蛋白17抗体
别 名 Zinc finger MIZ domain-containing protein 1; MIZ; PIAS like protein hZimp10; PIAS like protein on chromosome 10; PIAS like protein Zimp10; PIAS-like protein Zimp10; RAI 17; RAI17; RAI-17; Retinoic acid induced 17; Retinoic acid induced protein 17; Retinoic acid-induced protein 17; TRAFIP10; Zimp10; Zinc finger containing Miz1 PIAS like protein on chromosome 10; Zinc finger MIZ domain-containing protein 1; Zinc finger MIZ-type containing 1; Zmiz1; ZMIZ1_HUMAN; hZIMP10; KIAA1224.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep
产品类型 一抗
研究领域 细胞生物 细胞周期蛋白 转录调节因子 表观遗传学
蛋白分子量 predicted molecular weight: 115kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Retinoic acid induced 17
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation.
Tissue specificity:Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.
Function : Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation.
Subunit : Interacts with AR, but not with ESR1, NR3C1, PGR, THRB nor VDR.
Subcellular Location : Nucleus speckle. Cytoplasm.
Tissue Specificity : Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.
Similarity : Contains 1 SP-RING-type zinc finger.
Database links : UniProtKB/Swiss-Prot: Q9ULJ6.3
RAN GTPase酶激活蛋白1抗体说明书储存温度:
存在于血清、组织培养上清液或腹水中的抗体在-20℃下能够长期储存。低温存放不会损伤抗体活性。抗体的工作液通常储存于4℃中。此温度下,抗体的活性能稳定数月至数年亦不损失。抗体溶液不应反复冻融,因为反复冻融将导致部分抗体失活并产生不需要的蛋白—蛋白凝聚物。抗体的凝聚能导致活性丧失。这是由于抗原结合位点的空间构象被破坏,或由于产生的不溶物被离心或过滤而除去。冷凝蛋白类的抗体不能存放于4℃。因为在4℃下此类抗体会发生沉淀。一些鼠源性IgG3亚类的抗体有此特性。如果抗体在4℃下发生沉淀,就应该存放于室温下的叠氮钠溶液中。
RAN GTPase酶激活蛋白1抗体说明书的分类:
按作用对象,可将其分为抗毒素、抗菌抗体、抗病毒抗体和亲细胞抗体(能与细胞结合的免疫球蛋白,如1型变态反应中的lgE反应素抗体,能吸附在靶细胞膜上);按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类;按抗体的来源,可 将其分为天然抗体和免疫抗体。抗体就是免疫球蛋白,是改变了的球蛋白分子。由特异性抗原刺激产生,抗体的产生是由于抗原侵入人体后引起各种免疫细胞相互作用,使淋巴细胞中的B细胞分化增殖而形成浆细胞,浆细胞可产生分泌抗体。
Ebselen (50 mg) 2-phenyl-1,2-benzisoselenazol-3(2H)-one; Ebselen
BAY-u9773 (25 ug) 4-[[(1R,2E,4E,6Z,9Z)-1-[(1S)-4-carboxy-1-hydroxybutyl]-2,4,6,9-pentadecatetraenyl]thio]-benzoic acid; BAY-u9773
PPOH (5 mg) 2-(2-propynyloxy)-benzenehexanoic acid; PPOH
Curcumin (50 g) 1,7-bis(4-hydroxy-3-methoxyphenyl)-1E,6E-heptadiene-3,5-dione; Indian Saffron|Turmeric yellow; Curcumin
AAPH (10 g) 2,2’-azobis-2-methyl-propanimidamide, dihydrochloride; AAPH
Eicosapentaenoic Acid methyl ester (500 mg) 5Z,8Z,11Z,14Z,17Z-eicosapentaenoic acid, methyl ester; EPA methyl ester; Eicosapentaenoic Acid methyl ester
Linoleoyl Ethanolamide-d4 (500 ug) N-(2-hydroxyethyl-1,1,2,2-d4)-9Z,12Z-octadecadienamide; Linoleoyl Ethanolamide-d4
JWH 018 2-hydroxyindole metabolite (10 mg) (2-hydroxy-1-pentyl-1H-indol-3-yl)(naphthalen-1-yl)-methanone; JWH 018 2-hydroxyindole metabolite
JWH 073 2’-naphthyl-N-(1-methylpropyl) isomer (1 mg) (1-(sec-butyl)-1H-indol-3-yl)(naphthalen-2-yl)-methanone; JWH 073 2’-naphthyl-N-(1-methylpropyl) isomer
JWH 081 6-methoxynaphthyl isomer (5 mg) (6-methoxy-1-naphthalenyl)(1-pentyl-1H-indol-3-yl)-methanone; JWH 166; JWH 081 6-methoxynaphthyl isomer
JWH 424 (10 mg) (8-bromonaphthalen-1-yl)(1-pentyl-1H-indol-3-yl)methanone; JWH 424γ-taxilin/LSR5 脂多糖相关反应蛋白5抗体 0.2mlIFRD1 干扰素相关发育调节蛋白1抗体(神经生因子诱导蛋白PC4)规格: 0.2ml
phospho-Histone H1.4(Thr18) 磷酸化组蛋白H1.4抗体规格: 0.1mlPlastin L 淋巴细胞胞浆蛋白LCP1抗体规格: 0.2ml
FAM160B1 FAM160B1蛋白抗体规格: 0.2mlOtoraplin/OTOR/Fibrocyte derived protein 纤维细胞源性蛋白规格: 0.2ml
TLR11 Toll样受体11抗体规格: 0.2mlPhospho-GRB10 (Tyr67) 磷酸化生因子受体结合蛋白10抗体规格: 0.1ml
RAN GTPase酶激活蛋白1抗体说明书API2 凋亡抑制因子2抗体规格: 0.2mlGRIM19 干扰素/维甲酸诱导凋亡相关基因抗体规格: 0.1ml
Mouse Anti-rabbit IgG/Alexa Fluor 555 Alexa Fluor 555标记的小鼠抗兔IgG规格: 0.1mlGlycogen synthase 1 葡萄糖合成酶1抗体规格: 0.2ml
LAMP-3/DC-LAMP/CD208 溶酶体相关膜蛋白3抗体规格: 0.1mlPhospho-HER3(Tyr1328) 磷酸化HER3受体抗体规格: 0.1ml
γ-Catenin γ-连环素/连接蛋白γ抗体规格: 0.2mlIGFR3/CD16 FC段γ受体3/免疫球蛋白G Fc段受体III抗体规格: 0.1ml
Phospho-p90RSK(Ser380) 磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体规格: 0.1mlPhospho-MAP3K8/Tpl2 (Ser400) 磷酸化丝裂原活化蛋白激酶激酶8抗体规格: 0.1ml
DKK1 抑蛋白DKK1抗体规格: 0.2mlphospho-MAP4K4(Ser801) 磷酸化丝裂原活化蛋白激酶MAP4K4抗体规格: 0.1ml
CCDC147 卷曲螺旋结构域蛋白147抗体规格: 0.2mlMLX 转化因子样蛋白4抗体规格: 0.2ml
RAN GTPase酶激活蛋白1抗体说明书按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类。
IgM抗体是免疫应答中首先分泌的抗体。它们在与抗原结合后启动补体的级联反应。它们还把入侵者相互连接起来,聚成一堆便于巨噬细胞的吞噬;
IgG抗体激活补体,中和多种毒素。IgG持续的时间长,是 唯一能在母亲妊娠期穿过胎盘保护胎儿的抗体。他们还从乳腺分泌进入初乳,使新生儿得 到保护;
IgA抗体进入身体的黏 膜表面,包括呼吸、消化、生殖等管道的黏膜,中和感染因子。还可以通过母乳的初乳把这种抗体输送到新生儿的消化道黏膜中,是在母乳中含量最多,最为重要的一类抗体;
IgE抗体的尾部与嗜碱细胞、肥大细胞的细胞膜结合。当抗体与抗原结合后,嗜碱细胞与肥大细胞释放组织胺一类物质促进炎症的发展。这也是引发速发型过敏反应的抗体;
IgD抗体的作用还不太清楚。它们主要出现在成熟的B淋巴细胞表面上,可能与B细胞的分化有关。(IgD于1995年从人骨髓瘤蛋白中发现,分子量为175kD,主要由扁桃体、脾等处浆细胞产生,人血清中IgD浓度为3~40μg/ml,不到血清总Ig的1%,在个体发育中合成较晚。IgD铰链区很长,且对蛋白酶水解敏感,因此IgD半衰期很短,仅2.8天。血清中IgD确切的免疫功能尚不清楚。在B细胞分化到成熟B细胞阶段,除了表达SmIgD,抗原刺激后表现为免疫耐受。成熟B细胞活化后或者活化后或者变成记忆B细胞时,SmIgD逐渐消失。
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 26S 蛋白酶体 (26S PSM)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 26S PSM 单抗包被于酶标板上,标准品和样品中的 26S PSM 与单抗结合,加入生物素化的抗人 26S PSM
The GTPase RAN, the regulators of its nucleotide-bound state, and its effectors represent a specialized network in the RAS GTPase superfamily and regulate the localization of macromolecules (RNAs and proteins) in subcellular
High-resolution scanning electron microscopy provides three-dimensional surface images of nuclear pore complexes (NPCs) embedded in the nuclear envelope. Here, we describe a method for exposing the nuclear surface in mammalian
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