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- PRO-517
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- 2025年07月12日
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- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Actin
- 保质期：
1年
- 供应商：
上海沪震实业有限公司
- 保存条件：
-20°C
- 规格：
10µg/50µg/1mg
CATALOGUE NUMBER
PRO-517
INTRODUCTION
Actin is a muscle protein localized in the I band of the myofibrils; acting along with myosin, it is responsible for contraction and relaxation of muscle. Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or magnesium ions, as well as several low affinity sites. Actin exists as a monomer in low salt concentrations, but filaments form rapidly as salt concentration rises, with the consequent hydrolysis of ATP. It occurs in globular (G-actin) and fibrous (F-actin) forms. Actin is found in all eukaryotic cells (except for nematode sperm). Actin is one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Its other functions include cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.DESCRIPTION
Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle.The purification method used (according to Spudich & Watts) results in a highly purified protein having a Molecular mass of 43,000 dalton.
SOURCE
Rabbit Muscle.PHYSICAL APPEARANCE
Sterile Filtered White lyophilized (freeze-dried) powder.FORMULATION
The protein was lyophilized from a 1mg/ml solution containing 10mM Tris/HCl buffer pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT and 0.5% (w/v) SDS.SOLUBILITY
It is recommended to reconstitute the lyophilized Actin in sterile 18MΩ-cm H2O not less than 1mg/ml, which can then be further diluted to other aqueous solutions.
STABILITY
Lyophilized Actin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Actin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
PURITY
Greater than 98.0% as determined by SDS-PAGE.USAGE
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文献和实验Isolation of Actin and Myosin Filaments
LEVEL III Materials Relaxing Solution 0.1 M KCl 0.001 M MgCl 5 mM ATP 0.016 M NaH PO Na HPO Adjust pH to 7.3 0.05 M Sodium phosphate buffer, pH
Isolation of Actin and Myosin Filaments
LEVEL III Materials Relaxing Solution 0.1 M KCl 0.001 M MgCl 5 mM ATP 0.016 M NaH PO Na HPO Adjust pH to 7.3 0.05 M Sodium phosphate buffer, pH
dingxiangyua 有哪位高手知道F-GFP和GFP-actin的抗性分别是什么?急求 Fasta921 定位在膜上的GFP （F-GFP） 绿荧光蛋白(GFP)标记肌动蛋白(GFP-Actin) 抗性？不明白！ biyuntian478 lz是啥意思？ 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验
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