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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Hepatitis B Surface Antigen, adw2 Recombinant
- 保质期:
1年
- 供应商:
上海沪震实业有限公司
- 保存条件:
-20°C
- 规格:
10µg/50µg/1mg
CATALOGUE NUMBER
HBS-885
INTRODUCTION
HBsAg is the surface antigenof the Hepatitis-B-Virus (HBV). The capsidof a virus has different surface proteins from the rest of the virus. The antigen is a protein that binds specifically on one of these surface proteins. It is commonly referred to as the Australian Antigen.DESCRIPTION
HbsAg subtype adw2 produced in E.Coli, is a single, non-glycosylated, polypeptide chain containing 226 amino acids and having a molecular weight of approximately 23.0kDa. HBsAg adw2 migrates on SDS-PAGE as a 23kDa monomer band with minor amounts of dimer (46kDa) and trimer (69kDa) forms.The HBsAg is fused to a 6 His tag on C-terminus and purified by proprietary chromatographic techniques.
SOURCE
Escherichia Coli.PHYSICAL APPEARANCE
Sterile filtered colorless solution.FORMULATION
Sterile filtered solution containing 50mM arginine and 1M urea.STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
Greater than 90.0% as determined by SDS-PAGE.USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验在本月的欧洲肝病研究学会的官方期刊《Journal of Hepatology》上刊登了一篇香港学者关于乙型肝炎表面抗原(HBsAg)的综述性文章,该文章详细论述了目前HBsAg的最新进展以及在临床治疗和疾病预后中的意义。 图 HBV 生命周期 已经证实HBsAg定量检测有助于对HBV感染者的管理,但检测方法比较麻烦。最近,商业定量检测试剂盒的推广应用再次使人们的目光注意到血清HBsAg定量检测是一种有用
以已知抗体(抗HBs)包被载体,然后将含有抗原的待检标本加入,共同孵育后,抗原结合于包被抗体上,再加入酶标记特异抗体(酶标抗HBs),酶标抗体连接到抗原上,最后加入底物溶液,根据颜色反应来判定抗原含量。一、材料HBsAg试剂盒,本试剂盒含:1、包被抗-HBs反应条1瓶2、酶结合物1瓶3、HBsAg阳性对照1瓶4、HBsAg阴性对照1瓶5、洗涤液:用前每瓶作1:20稀释1瓶6、显色剂(TMB)A1瓶7、显色剂(TMB)B1瓶8、终止液1瓶二、方法1、加入待测标本每孔0.05ml,并设HBsAg
g阳性对照 1瓶 4、HBSA g阴性对照 1瓶 5、洗涤液:用前每瓶作1:20稀释 1瓶 6、显色剂(TMB)A 1瓶 7、显色剂(TMB)B 1瓶 8、终止液 1瓶 【方法】 1、加入待测标本每孔0.05ml,并设HBSA g阳性对照2孔,HBsAg阴性对照2孔,空白对照1孔,然后加入酶结合物每孔1滴(0.05ml、空白对照孔不加
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