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- 技术资料
- 保存条件:
参照说明书
- 保质期:
参照说明书
- 英文名:
ImmPRESS*-AP ANTI-GOAT IgG (MADE IN HORSE) (15ml)
- 库存:
1
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
15 ml/50 ml
| 规格: | 15 ml | 产品价格: | ¥2440.0 |
|---|---|---|---|
| 规格: | 50 ml | 产品价格: | ¥6360.0 |
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
in a humid chamber for 1 hour, RT. Blot off antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of the secondary antibody (Horse anti-mouse IgG-Vector Catalog No. BA2001) in 1.0% BSA/PBS, 2.0% normal horse serum. Prepare
150µl of working dilution. Incubate sections in a humid chamber for 1 hour, RT. Blot off antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of the secondary antibody (Horse anti-mouse IgG-Vector Catalog No. BA2001) in 1.0%
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