COX4细胞色素c氧化酶IV亚型1单克隆抗体

英文名称COX4
中文名称细胞色素c氧化酶IV亚型1单克隆抗体
别    名COX-IV; COX 4; COX 4I1; COX 4I2; COXIV Isoform 2; COX IV 1; COX IV-1; COX IV; COX4 2; COX4; COX4B; COX4I1; COX4I2; COX4L2; COXIV 2; COXIV; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome C Oxidase Subunit IV; Cytochrome c oxidase subunit IV isoform 1; Cytochrome c oxidase subunit IV isoform 2 (lung); dJ857M17.2; MGC72016; COX IV-1; Cox4a Cytochrome c oxidase polypeptide IV; COX41_HUMAN; COX4I1.  
规格50ul  100ul  
研究领域细胞生物  免疫学  线粒体  
抗体来源Mouse
克隆类型Monoclonal
克 隆 号8D8
交叉反应 Human, Mouse, Rat, 
产品应用WB=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:200-800 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量17kDa
细胞定位细胞浆 细胞膜 线粒体
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原Recombinant human COX4: 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]

Function:
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Subcellular Location:
Mitochondrion inner membrane.

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the cytochrome c oxidase IV family.

SWISS:
P13073

Gene ID:
1327

Database links:
Entrez Gene: 1327 Human
Entrez Gene: 12857 Mouse
Entrez Gene: 29445 Rat
Entrez Gene: 281090 Cow
Omim: 123864 Human
SwissProt: P00423 Cow
SwissProt: Q3SZS0 Cow
SwissProt: P13073 Human
SwissProt: P19783 Mouse
SwissProt: P10888 Rat
Unigene: 433419 Human
Unigene: 386758 Mouse
Unigene: 2528 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

细胞色素c氧化酶4亚型1抗体；（细胞色素c氧化酶IV亚型1）是一种存在线粒体上的大型跨膜蛋白复合物。由于细胞色素氧化酶是呼吸电子传递链的第四个中心酶复合物，因此又被称为复合物IV。
 

产品图片

Sample: Jurkat Cell (Human) Lysate at 40 ug Primary: Anti- COX4 (bsm-33037M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 16 kD Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti- COX4 (bsm-33037M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 16 kD Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (bsm-33037M-8D8) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining.