产品概述:PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta.
靶点/蛋白:A synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen. This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recognized on the western blot gel.
来源:Rabbit
存储溶液:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:1. Yu, J., Zhang, L., Hwang, P.M., Kinzler, K.W. and Vogelstein, B., 2001. Mol. Cell 7, pp. 673 2. Nakano, K. and Vousden, K.H., 2001. Mol. Cell 7, pp. 683 3. Han, J. et al. 2001. Proc. Natl. Acad. Sci. USA 98, pp. 11318
简单描述:Rabbit Monoclonal PUMA Antibody (C-term). Validated for use in WB, IHC, IF. Clone: EP512Y.
详情:Function Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis. Cellular_Location Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release Tissue_Location Ubiquitously expressed.