产品概述:Interleukin enhancer-binding factor 3 (ILF3) is a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2 (1). ILF3 regulates protein arginine N-methyltransferase 1 activity and has been suggested to regulate transcription of the IL2 gene during T-cell activation. ILF3 promotes the formation of stable DNA-dependent
靶点/蛋白:A synthetic peptide corresponding to residues in human ILF3 was used as an immunogen.
来源:Rabbit
存储溶液:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:1. NCBI Reference Sequence (RefSeq): a curated non- redundant sequence database of genomes, transcripts and proteins Pruitt KD, Tatusova, T, Maglott DR Nucleic Acids Res 2007 Jan 1;35(Database issue):D61-5 2. The UniProt Consortium, The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008)
特异性:Human
别名:Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, DRBP76, M-phase phosphoprotein 4, MPP4, Nuclear factor associated with dsRNA, NFAR, Nuclear factor of activated T-cells 90 kDa, NF-AT-90, Translational control protein 80, TCP80, ILF3, DRBF, MPHOSPH4, NF90
应用:WB~~1:20000~100000IHC~~1:250~500
简单描述:Rabbit Monoclonal ILF3 Antibody. Validated for use in WB, IHC, IF. Clone: EPR3627.
详情:FunctionMay facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N- methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication (By similarity).Cellular_LocationNucleus, nucleolus. Cytoplasm. Nucleus. Note=Localizes in the cytoplasm in response to viral dsRNA. The unphosphorylated form is retained in the nucleus by ILF2 Phosphorylation at Thr-188 and Thr-315 causes the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3. Localized in cytoplasmic mRNP granules containing untranslated mRNAsTissue_LocationUbiquitous.