产品概述:Novel homeobox gene, denoted TGIF (5TG3' interacting factor), belongs to an expanding TALE (three amino acid loop extension) superclass of atypical homeodomains. The TGIF homeodomain binds to a previously characterized retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE), which contains an unusual DNA target for a homeobox (1). In vitro studies have implicated TGIF as a transcriptional repressor and corepressor in retinoid and TGF-beta signaling pathways that regulate several important biological processes. Heterozygous nonsense and missense mutations of the human TGIF gene have been associated with holoprosencephaly, the most common congenital malformation of the forebrain (2). It has been reported that TGIF plays an unexpected role in the inhibition of Smad2 phosphorylation, which occurs by a mechanism independent of its association with Smad2. This inhibitory function of TGIF is executed in concert with c-Jun, which facilitates the interaction of TGIF with cPML, resulting in the nuclear sequestration of cPML and the disruption of the cPML-SARA complex (3).
靶点/蛋白:A synthetic peptide corresponding to residues on the C-terminus of human TGIF was used as an immunogen. This antibody detects both phosphorylated and unphosphorylated TGIF.
来源:Rabbit
存储溶液:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:1. Bertolino E, et al. J Biol Chem 270(52):31178-88, 1995.
2. Shen J, et al. Mol Cell Biol 25(9):3639-47, 2005.
3. Seo SR, et al. Mol Cell, 23(4):547-59, 2006.
特异性:Human
别名:Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1, TGIF1, TGIF
应用:WB~~1:1000
简单描述:Rabbit Monoclonal TGIF Antibody (C-term). Validated for use in WB. Clone: EP637Y.
详情:FunctionBinds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII- RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.Cellular_LocationNucleus.