靶点/蛋白:Hamster anti-Mouse CD81 antibody, clone Eat2 recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti-Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti-Ig and IL-4 induced proliferation (Maeckeret al.2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubensteinet al.2006).
来源:Hamster
参考文献:1. Clark, K. et al. (2001) PGRL is a major CD81 - associated protein on lymphocytes and distinguishes a new family of cell surface proteins. J. Immunol. 167: 5115 - 5121. 2. Maecker, H.T. et al. (2000) Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies.Hybridoma 19: 15-22. 3. Conde-Vancells, J. et al. (2010) Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.Proteomics Clin Appl. 4: 416-425. 4. Conde-Vancells, J. et al. (2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.J Proteome Res. 7: 5157-66. 5. Takeda, Y. et al. (2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.J Biol Chem. 283: 26089-97. 6. Suzuki, M. et al. (2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.J Immunol. 182: 6485-93. 7. Ha, C.T. et al. (2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1.J Leukoc Biol. 77: 948-57. 8. Pan, Q. et al. (2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi).Gut. 61: 1330-9. 9. Jin, Y. et al. (2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.PLoS One. 8: e73706. 10. Royo, F. et al. (2013) Transcriptome of extracellular vesicles released by hepatocytes.PLoS One. 8: e68693. 11. Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.Cancer Res. 61: 5248-54.
特异性:Mouse
别名:CD81 antigen, 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, CD81, Cd81, Tapa1
简单描述:Hamster Monoclonal Anti-Mouse CD81 Antibody, clone Eat2 (RPE). Validated for use in WB, IHC-F, FC, E, IP. Clone: Eat2.