产品概述:The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion; in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). As a filamentous (F) actin crosslinking protein, MARCKS binds to actin, calmodulin, and synapsin. In murine, MARCKS binding activity is regulated by PKC phosphorylation at Ser152, 156, and 163 of MARCKS during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion. After phosphorylation, MARCKS is redistributed from plasma membrane to cytoplasm and is involved in leukocyte motility. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).
靶点/蛋白:A synthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein. The orthologous phosphorylation sites in mouse and rat is serine 152 and 156
来源:Rabbit
存储溶液:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:1. Harlan DM, et al. J Biol Chem 266(22):14399-405, 1991
2. Hartwig JH, et al. Nature 356(6370):618-22, 1992.
特异性:Human, Mouse, Rat
别名:Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL, MARCKS, MACS, PRKCSL
应用:WB~~1:1000~2000
简单描述:Rabbit Monoclonal MARCKS Antibody Phospho (pS159/S163). Validated for use in WB. Clone: EP2113Y.
详情:FunctionMARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.Cellular_LocationCytoplasm, cytoskeleton. Membrane; Lipid-anchor