产品概述:Ras, a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4).
靶点/蛋白:A synthetic peptide corresponding to residues in the effector region of human Ras was used as an immunogen. Predicted to react with other Ras family members and cross-react with most species based on sequence homology.
来源:Rabbit
存储溶液:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:1. Ellis RW, et al. Nature. 1981 Aug 6; 292(5823):506
2. Campbell, S. L., (1998) Oncogene 17, 1395-1413
3. Zheng, Y., and Quilliam, L. A. (2003) EMBO Rep 4, 463-468
4. Bos JL. Cancer Res. 1989; 49:4682
特异性:Human
别名:GTPase NRas, Transforming protein N-Ras, NRAS, HRAS1
应用:WB~~1:2000~10000
简单描述:Rabbit Monoclonal p21/Ras Antibody. Validated for use in WB, IHC. Clone: Y131.
详情:FunctionRas proteins bind GDP/GTP and possess intrinsic GTPase activity.Cellular_LocationCell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus