产品概述:Collagenase-3 (MMP13), a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, is expressed in the skeleton during embryonic development and is highly overexpressed in human carcinomas and in chondrocytes and synovial cells in rheumatoid arthritis and osteoarthritis. Findings demonstrate a unique role of Mmp13 in skeletal development (1). In arthritis, the destruction of fibrillar (type II) collagen is one of the hallmarks, with MMP-1 and MMP-13 being identified as key players in arthritic cartilage. MMP-13, furthermore, has been found in highly metastatic tumors. In contrast to many human MMPs, the MMP-13 distribution pattern is restrictive in normal tissues and selective in pathological conditions. MMP-13 demonstrates versatility in its substrate utilization. In addition to being highly active on type II collagen, MMP-13 cleaves other substrates, mostly macromolecules of the extracellular matrix, but also molecules such as connective tissue (CTGF) and fibrinogen. MMP-13 is controlled at multiple levels: i.e., the expression/synthesis, activation, and inhibition of the active enzyme (3).
靶点/蛋白:A synthetic peptide corresponding to residues near the C-terminus of human MMP-13 was used as an immunogen.
来源:Rabbit
存储溶液:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:1. Inada M, et al. Proc Natl Acad Sci USA 101(49):17192-7, 2004
2. Maskos K, et al. J Mol Biol 366(4):1222-31, 2007.
3. Tardif G, et al. Mod Rheumatol 14(3):197-204, 2004
简单描述:Rabbit Monoclonal MMP-13 Antibody. Validated for use in WB. Clone: EP1263Y.
详情:FunctionPlays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.Cellular_LocationSecreted, extracellular space, extracellular matrix. SecretedTissue_LocationDetected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue.