产品概述:Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
靶点/蛋白:Lymphoblastoid cell line Daudi
来源:Mouse
存储溶液:0.5 ml at 200ug/ml; Conjugated to PE
参考文献:1. Schlossman S, et al. (eds). Leukocyte Typing V, Oxford University Press, Oxford, p511-515, 1995.2. Tedder TF and Schlossman SF. Phosphorylation of the B1 (CD20) molecule by normal and malignant human B lymphocytes. J Biol Chem 1988, 263(20):10009-10015.3. Bubien JK et al. Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes. J Cell Biol 1993, 121(5):1121-1132.4. Tedder TF and Engel P. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol Today 1994, 15(9):450-454.5. Kanzaki M et al. Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells. J Biol Chem 1995, 270(22):13099-13104.
简单描述:Mouse Monoclonal CD20 Antibody [Clone B9E9]. Validated for use in FC. Clone: B9E9.
详情:FunctionThis protein may be involved in the regulation of B-cell activation and proliferation.Cellular_LocationCell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchorTissue_LocationExpressed on B-cells.