产品概述:Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
靶点/蛋白:A synthetic phospho-peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8 was used as immunogen. The antibody only detects Keratin 8 phosphorylated on Serine 73.
来源:Rabbit
存储溶液:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:1. Ku, N.O. and M.B. Omary. Identification and mutational analysis of the glycosylation sites of human keratin 18. J Biol Chem. 270: 11820
2. Kulesh, D.A. and R.G. Oshima. Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells. Mol Cell Biol. 8: 1540
3. Marceau, N. and A. Loranger. Cytokeratin expression, fibrillar organization and subtle function in liver cells. Biochem. Cell Biol. 73: 619
4. Fuchs, E. Keratins and the skin. Annu. Rev. Cell. Dev. Biol. 11: 123
特异性:Human, Mouse
别名:Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
应用:WB~~1:5000IHC~~1:500
简单描述:Rabbit Monoclonal Cytokeratin-8 Antibody Phospho (pS73). Validated for use in WB, IHC, IF. Clone: E431-2.
详情:FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.Cellular_LocationCytoplasm. Nucleus, nucleoplasm Nucleus matrixTissue_LocationObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.