- $50 - 2700
- prospecbio
- ENZ-553
- 以色列
- 2025年07月13日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Luciferin 4-Monooxygenase Firefly Recombinant
- 保质期：
1年
- 供应商：
上海沪震实业有限公司
- 保存条件：
-20°C
- 规格：
5μg/20μg/1mg
CATALOGUE NUMBER
ENZ-553
SYNONYMS
Luciferase-like monooxygenase, LUC, EC 1.13.12.7.INTRODUCTION
Luciferase is a general term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which involves the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is broadly used as a reporter for studying gene regulation and function, and for pharmaceutical screening.DESCRIPTION
Luciferase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 571 amino acids (1-550 a.a.) and having a molecular mass of 62.9kDa.Luciferase is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SOURCE
Escherichia Coli.PHYSICAL APPEARANCE
Sterile filtered colorless solution.FORMULATION
The Luciferase protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH-8), 1mM DTT, and 10% glycerol.STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
Greater than 90.0% as determined by SDS-PAGE.AMINO ACID SEQUENCE
MGSSHHHHHH SSGLVPRGSH MMEDAKNIKK GPAPFYPLED GTAGEQLHKA MKRYALVPGT IAFTDAHIEV DITYAEYFEM SVRLAEAMKR YGLNTNHRIV VCSENSLQFF MPVLGALFIG VAVAPANDIY NERELLNSMG ISQPTVVFVS KKGLQKILNV QKKLPIIQKI IIMDSKTDYQ GFQSMYTFVT SHLPPGFNEY DFVPESFDRD KTIALIMNSS GSTGLPKGVA LPHRTACVRF SHARDPIFGN QIIPDTAILS VVPFHHGFGM FTTLGYLICG FRVVLMYRFE EELFLRSLQD YKIQSALLVP TLFSFFAKST LIDKYDLSNL HEIASGGAPL SKEVGEAVAK RFHLPGIRQG YGLTETTSAI LITPEGDDKP GAVGKVVPFF EAKVVDLDTG KTLGVNQRGE LCVRGPMIMS GYVNNPEATN ALIDKDGWLH SGDIAYWDED EHFFIVDRLK SLIKYKGYQV APAELESILL QHPNIFDAGV AGLPDDDAGE LPAAVVVLEH GKTMTEKEIV DYVASQVTTA KKLRGGVVFV DEVPKGLTGK LDARKIREIL IKAKKGGKIA V.USAGE
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验【求助】topflash/foplash的luciferase opening reading fream针对的就是firefly萤光素酶吗？
orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. 这里的Luciferase没有标明是Firefly的啊，那我要不要再转一个PGL3载体呢？？？ 在线等答案，谢谢啦！ paroxysm 荧光素酶已经带上了，但是不是firefly种属，看说明书上说的。 但kit里的底物应该是针对这个荧光素酶的。
Improvements in the Application of Firefly Luciferase Assays
The firefly luciferase-based assay differs from most familiar enzyme-based determinations. Most enzyme assays are based either on the production of a product or the disappearance of a substrate. Usually the compound measured is stable
Application of the Firefly Luciferase Reporter Gene
) as a reporter gene proved to be both laborious and onerous. In contrast, the luciferase gene possesses intrinsic qualities that most other reporter genes lack greater sensitivity, ease of use, instantaneous quantification, “ environment friendliness
技术资料暂无技术资料 索取技术资料
