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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
96T
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文献和实验must be incorporates into the test to identify these potential problems in ELISA. The enzyme catalyzes a change in a substrate molecule, but the enzyme itself is not consumed in the process. The enzyme molecule continues to act on more substrate molecules and produce
The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites, capable of binding to antibody, since at least two antibodies act in the sandwich
0.3ml enzyme (alkaline phosphatase, Sigma type VII, sp act. 1,140 U/ml) at 8,000 x g for 2 mins. 2. Add to the pellet 0.1 ml specific antibody (animal specific, pathogen specific) affinity purified, resulting in a 3:1 ratio of enzyme to antibody.
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