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    Human C-Reactive Protein (CRP) ELISA Kit

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    Human CRP ELISA Kit

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    • 入驻年限  2年
    • 北京市朝阳区小营路10号阳明广场南楼9B1
    • 货号: 1000
      供应商: 北京博蕾德生物科技有限公司
      数量: 1
      规格: 1 kit
      ADI公司位于美国德克萨斯州的圣安东尼奥市,提供的主要客户服务如肽合成和多克隆抗体。ADI公司在生产和发展中专营多种多样人和动物的酶联免疫试剂和试剂盒供研究使用。ADI公司引进了大量专业的抗体包括:肥胖基因、瘦素基因、膜受体和神经递质转运剂等。

      Introduction  
      C-reactive protein (CRP) has been  regarded as an acute phase reactant in serum.  It 
      consists of five single subunits, which noncovalently linked and assembled, as a cyclic 
      pentamer with a mol. Wt. Range of 110-140 kDa.  CRP has been found to be increased in 
      serum of patients with a wide variety of diseases including infections by gram-positive and 
      gram-negative bacteria, acute phase of rheumatoid arthritis, abdominal abscesses, 
      inflammation of bile ducts (4), myocardial infarction, and malignant tumors.  CRP may be 
      found in patients with Guillain-Barre syndrome and multiple sclerosis, certain viral 
      infections, tuberculosis, acute infectious hepatitis, many other necrotic and inflammatory 
      diseases, burned patients, and after surgical trauma.  Although the detection of elevated 
      levels of CRP in the serum is not specific for any particular disease, it is useful indicator of 
      inflammatory processes.  CRP levels rise in serum within hours of the onset of 
      inflammation, reach a peak during the acute stage and decrease with resolution of 
      inflammation  trauma.  The detection of CRP is a more reliable and sensitive indicator of 
      the inflammatory process than the erythrocyte sedimentation rate, which may also be 
      influenced by physiological changes not associated with an inflammation process.  Current 
      quantification methods including latex agglutination, nephelometry, radial immunodiffusion 
      have the general disadvantage accompany agglutination and precipitation techniques.   
       
      ADI’s CRP ELISA provides is a very specific and sensitive assay for CRP.   


      PRINCIPLE OF THE TEST 
       
      Human CRP ELISA kit is based on simultaneous binding of human CRP from samples to 
      two antibodies, one immobilized on the microtiter well plates, and other conjugated to the 
      enzyme horseradish peroxidase.  After a washing step, chromogenic substrate is added 
      and colors developed.  The enzymatic reaction (color) is directly proportional to the amount 
      of CRP present in the sample.  Adding stopping solution terminates the reaction.  
      Absorbance is then measured on a microtiter well ELISA reader at 450 nm. and the 
      concentration of CRP in samples and control is read off the standard curve. 
       
      MATERIALS AND EQUIPMENT REQUIRED 
       
      Adjustable micropipet (5-1000 ul) and multichannel pipet with disposable plastic tips.  
      Reagent troughs, plate washer (recommended) and ELISA plates Reader. 
       
      PRECAUTIONS AND SAFETY INSTRUCTIONS 
       
      ADI CRP ELISA kit is intended for in vitro research use only.  The reagents contain prolcin-
      100  (0.1%) as preservative; necessary care should be taken when disposing solutions.  
      The stds./controls sera contain human serum that has been shown to be negative for 
      HbsAg, HCV, and HIV antibodies.  Nevertheless, such tests are unable to prove the 
      complete absence of viruses, therefore, sera should be handled at biosafety level 2, as 
      recommended for any potentially infectious human serum or blood specimen in the 
      CDC/NIH Manual, “Biosafety in microbiological and biomedical laboratories, 1984”.  
       
      Applicable MSDS, if not already on file, for the following reagents can be obtained from 
      ADI or the web site. 
       
      TMB (substrate), H2SO4 (stop solution), and Prolcin-300 (0.1% v/v in standards, sample 
      diluent and HRP-conjugates).   All waste material should be properly disinfected before 
      disposal.  Avoid contact with the stop solution (1N sulfuric acid).   
       
      SPECIMEN COLLECTION AND HANDLING 

       
      Collect blood by venipuncture, allow clotting, and separating the serum by centrifugation at 
      room temperature.  Do not heat inactivate the serum..  Do not add azide or other 
      preservatives.  If sera cannot be immediately assayed , these could be stored at -20oC for 
      up to six months.  Avoid repeated freezing and thawing of samples.  No preservatives 
      should be added to the serum.  This kit has not been optimized for plasma, urine, or saliva 
      culture medium.  Users must optimized the assa

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      资料下载:

      1000-Human-C-Reactive-Protein-CRP-ELISA-kit-manual (1).pdf 附件下载 (下载 1 次)

    • Human C-Reactive Protein (CRP) ELISA Kit

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