首页 / ELISA 试剂盒 / 人ELISA试剂盒 / Human C-Reactive Protein (CRP) ELISA Kit

我的询价

99
商家已回复你的询价 【点击查看】

询价列表

暂时没有已询价产品

当你希望让更多商家联系你时,可以勾选后发送询价,平台会将你的询价消息推荐给更多商家。
个人收货地址

编辑个人收货信息

Human C-Reactive Protein (CRP) ELISA Kit

收藏

Human CRP ELISA Kit

丁香通采购保障计划,让您的采购更放心。 查看详情

微信二维码询价

扫一扫微信联系

促销详情
促销信息
68折促销
备注
包邮。
倒计时
99000000

站内推广

  • 商家诚信度 
  • 入驻年限  2年
  • 北京市朝阳区小营路10号阳明广场南楼9B1
  • 货号: 1000
    供应商: 北京博蕾德生物科技有限公司
    数量: 1
    规格: 1 kit
    ADI公司位于美国德克萨斯州的圣安东尼奥市,提供的主要客户服务如肽合成和多克隆抗体。ADI公司在生产和发展中专营多种多样人和动物的酶联免疫试剂和试剂盒供研究使用。ADI公司引进了大量专业的抗体包括:肥胖基因、瘦素基因、膜受体和神经递质转运剂等。

    Introduction  
    C-reactive protein (CRP) has been  regarded as an acute phase reactant in serum.  It 
    consists of five single subunits, which noncovalently linked and assembled, as a cyclic 
    pentamer with a mol. Wt. Range of 110-140 kDa.  CRP has been found to be increased in 
    serum of patients with a wide variety of diseases including infections by gram-positive and 
    gram-negative bacteria, acute phase of rheumatoid arthritis, abdominal abscesses, 
    inflammation of bile ducts (4), myocardial infarction, and malignant tumors.  CRP may be 
    found in patients with Guillain-Barre syndrome and multiple sclerosis, certain viral 
    infections, tuberculosis, acute infectious hepatitis, many other necrotic and inflammatory 
    diseases, burned patients, and after surgical trauma.  Although the detection of elevated 
    levels of CRP in the serum is not specific for any particular disease, it is useful indicator of 
    inflammatory processes.  CRP levels rise in serum within hours of the onset of 
    inflammation, reach a peak during the acute stage and decrease with resolution of 
    inflammation  trauma.  The detection of CRP is a more reliable and sensitive indicator of 
    the inflammatory process than the erythrocyte sedimentation rate, which may also be 
    influenced by physiological changes not associated with an inflammation process.  Current 
    quantification methods including latex agglutination, nephelometry, radial immunodiffusion 
    have the general disadvantage accompany agglutination and precipitation techniques.   
     
    ADI’s CRP ELISA provides is a very specific and sensitive assay for CRP.   


    PRINCIPLE OF THE TEST 
     
    Human CRP ELISA kit is based on simultaneous binding of human CRP from samples to 
    two antibodies, one immobilized on the microtiter well plates, and other conjugated to the 
    enzyme horseradish peroxidase.  After a washing step, chromogenic substrate is added 
    and colors developed.  The enzymatic reaction (color) is directly proportional to the amount 
    of CRP present in the sample.  Adding stopping solution terminates the reaction.  
    Absorbance is then measured on a microtiter well ELISA reader at 450 nm. and the 
    concentration of CRP in samples and control is read off the standard curve. 
     
    MATERIALS AND EQUIPMENT REQUIRED 
     
    Adjustable micropipet (5-1000 ul) and multichannel pipet with disposable plastic tips.  
    Reagent troughs, plate washer (recommended) and ELISA plates Reader. 
     
    PRECAUTIONS AND SAFETY INSTRUCTIONS 
     
    ADI CRP ELISA kit is intended for in vitro research use only.  The reagents contain prolcin-
    100  (0.1%) as preservative; necessary care should be taken when disposing solutions.  
    The stds./controls sera contain human serum that has been shown to be negative for 
    HbsAg, HCV, and HIV antibodies.  Nevertheless, such tests are unable to prove the 
    complete absence of viruses, therefore, sera should be handled at biosafety level 2, as 
    recommended for any potentially infectious human serum or blood specimen in the 
    CDC/NIH Manual, “Biosafety in microbiological and biomedical laboratories, 1984”.  
     
    Applicable MSDS, if not already on file, for the following reagents can be obtained from 
    ADI or the web site. 
     
    TMB (substrate), H2SO4 (stop solution), and Prolcin-300 (0.1% v/v in standards, sample 
    diluent and HRP-conjugates).   All waste material should be properly disinfected before 
    disposal.  Avoid contact with the stop solution (1N sulfuric acid).   
     
    SPECIMEN COLLECTION AND HANDLING 

     
    Collect blood by venipuncture, allow clotting, and separating the serum by centrifugation at 
    room temperature.  Do not heat inactivate the serum..  Do not add azide or other 
    preservatives.  If sera cannot be immediately assayed , these could be stored at -20oC for 
    up to six months.  Avoid repeated freezing and thawing of samples.  No preservatives 
    should be added to the serum.  This kit has not been optimized for plasma, urine, or saliva 
    culture medium.  Users must optimized the assa

    资料下载:

    1000-Human-C-Reactive-Protein-CRP-ELISA-kit-manual (1).pdf 附件下载 (下载 1 次)

  • Human C-Reactive Protein (CRP) ELISA Kit

    * 联系人
    * 电话
    * 验证码
    换一个

    填写更多信息,方便商家更快联系你 展开

    采购截止时间
    数量
    单位
    单位/学校
    职位
    地址
    详细地址
    邮箱
    0/120

询价发送成功

会在1个工作日内联系您!

马上登录注册,即可保存询价历史

你可能感兴趣的产品