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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Macrophages / Monocytes Monoclonal Antibody (MOMA-2)
- 靶点:
Macrophages / Monocytes
- 浓度:
0.5 mg/ml
- 应用范围:
Flow Cytometry, Immunofluorescence, Immunohistochemistry (Frozen)
- 宿主:
Rat
- 适应物种:
Mouse
- 克隆性:
Monoclonal
- 保存条件:
4°C or -20°C if preferred
- 形态:
Liquid
- 亚型:
IgG2b
- 免疫原:
Murine lymph node stroma.
- 规格:
250 µg
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文献和实验Monocytes/Macrophages: Enrichment of PBMCs with Monocytes
This protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70 monocytes. This cell suspension is than used for positive or negative MACS
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
Monoclonal Antibody Production Protocol
of debris, rounded and refractive under phase contrast, and that they are harvested in log or late log phase growth (between 3.5 and 9 x105 cells/ml). Thaw cells 7 days before scheduled fusion. Myeloma do not grow well after being in culture
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