- 询价
- invitrogen
- MA119490
- 2025年12月16日
- Flow Cytometry
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
SSEA1 Monoclonal Antibody (MEM-158), Biotin
- 抗体英文名：
SSEA1 Monoclonal Antibody
- 靶点：
SSEA1
- 浓度：
1 mg/ml
- 应用范围：
Flow Cytometry
- 宿主：
Mouse
- 适应物种：
Human
- 克隆性：
Monoclonal
- 保存条件：
4° C, do not freeze
- 形态：
Liquid
- 亚型：
IgM
- 免疫原：
Human granulocytes.
- 规格：
100 µg
产品详细信息
This antibody will not cross-react with porcine, bovine or sheep.
靶标信息
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
SSEA1 Antibody (MA1-19490) in Flow
Separation of human neutrophil granulocytes (red-filled) from CD15 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD15 (MEM-158) Biotin Monoclonal antibody (Product # MA1-19490) (concentration in sample 2 µg/mL, Streptavidin APC)
SSEA1 Antibody (MA1-19490) in Flow
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (MEM-158) Biotin Monoclonal antibody (Product # MA1-19490) (concentration in sample 2 µg/mL, Streptavidin APC)
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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