- 询价
- invitrogen
- PA540096
- 2025年12月22日
- ChIP assay, ChIP-sequencing, Immunocytochemistry, Immunofluorescence, Peptide Array, Western Blot
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Acetyl-Histone H3 (Lys27) Polyclonal Antibody
- 靶点：
Acetyl-Histone H3 (Lys27)
- 浓度：
Lot-Specific
- 应用范围：
ChIP assay, ChIP-sequencing, Immunocytochemistry, Immunofluorescence, Peptide Array, Western Blot
- 宿主：
Rabbit
- 适应物种：
Human
- 标记物：
Acetylated: Lys27
- 克隆性：
Polyclonal
- 保存条件：
-20°C or -80°C if preferred
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH-conjugated synthetic peptide corresponding to human histone H3, acetylated at lysine 27 (H3K27ac).
- 规格：
50 µg
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer
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