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- prospecbio
- CYT-978
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- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
CD4 (26-396) Human Recombinant, Sf9
- 保质期：
1年
- 供应商：
上海沪震实业有限公司
- 保存条件：
-20°C
- 规格：
1μg/5μg/50μg
CATALOGUE NUMBER
CYT-978
SYNONYMS
CD4 Molecule, T-Cell Surface Antigen T4/Leu-3, CD4 Antigen (P55), T-Cell Surface Glycoprotein CD4, CD4 Receptor, CD4 Antigen, CD4mut.INTRODUCTION
CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.DESCRIPTION
CD4 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 377 amino acids (26-396a.a.) and having a molecular mass of 42.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).CD4 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
SOURCE
Sf9, Insect cells.PHYSICAL APPEARANCE
Sterile filtered colorless solution.FORMULATION
CD4 a protein solution (0.25mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
Greater than 95.0% as determined by SDS-PAGE.AMINO ACID SEQUENCE
KKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTKGPSK LNDRADSRRS LWDQGNFPLI IKNLKIEDSD TYICEVEDQK EEVQLLVFGL TANSDTHLLQ GQSLTLTLES PPGSSPSVQC RSPRGKNIQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ KASSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWITFD LKNKEVSVKR VTQDPKLQMG KKLPLHLTLP QALPQYAGSG NLTLALEAKT GKLHQEVNLV VMRATQLQKN LTCEVWGPTS PKLMLSLKLE NKEAKVSKRE KAVWVLNPEA GMWQCLLSDS GQVLLESNIK VLPTWSTPVQ PHHHHHH.USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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文献和实验CD26 分子 CD26 常用单克隆抗体或代号： 5.9,Tal 主要表达细胞： Ta,Ba,Mac [NL] 分子质量(kDa)和结构： gp110 功 能： 二肽酰酶Ⅳ(DPPⅣ),HIV另一类受体,参与T细胞活化,腺苷脱氨酶结合蛋白 CD26 Aka dipeptidylpeptidase IV, adenosine deaminase complexing protein
Production of Primary Human CD4+ T Cell Lines and Clones
Tetramer staining of CD4+ T cells is a valuable technique in immunology for detecting rare autoreactive T cells. Generating clones or cell lines from autoantigen tetramer-positive CD4+ T cells allows further characterization and phenotyping
Cell surface expression of recombinant olfactory receptors (ORs) is a major limitation in characterizing their functional nature. We have shown that the recombinant expression of a human OR, OR 17-210, in the baculovirus/Sf9
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