MEM + 20% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) We strongly suggest to purchase the complete medium from .
Cryopreservation medium:
90%FBS+10%DMSO
Genes Expressed:
The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.
Cellular Products:
neurofilament proteins
Tumor Formation:
Yes, in nude mice; forms serially transplantable intercranial and subcutaneous tumors Yes, in soft agar
Comments:
The cells produce tumors in nude mice. The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody. The c-myc oncogene is amplified in D341 Med cells.