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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Rhesus Monkey IgG Antigen ELISA Kit
- 保质期：
1年
- 供应商：
上海钰博生物科技有限公司
- 保存条件：
-2-8℃
- 规格：
盒
Rhesus Monkey IgG Antigen ELISA Kit
Product Description
Rhesus Monkey IgG Antigen ELISA Kit
This rhesus macaque (Macaca mulatta) monkey Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total IgG antigen in rhesus monkey serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses.Rhesus monkey IgG will bind to the monoclonal capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled monoclonal anti-rhesus IgG primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rhesus monkey IgG. Color development is proportional to the concentration of IgG in the samples.
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文献和实验-human immunoglobulin (Ig)A, anti-human IgM and anti-human IgE were developed for the simultaneous capture of total IgG, IgA, IgM and IgE (IgGAME) antibodies from human or non-human primate serum. The fraction of antigen-specific antibodies captured
thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
Antigen Design Sera Purification Tech Sheet
. It has the capacity to bind at least two molecules of IgG. The binding is specific to the Fc portion and does not affect the antigen binding sites. Protein G is isolated from Group G streptococci and binds the Fc region of the IgG in a similar manner to Protein
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