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- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Rat Ceruloplasmin ELISA Kit
- 保质期：
1年
- 供应商：
上海钰博生物科技有限公司
- 保存条件：
-2-8℃
- 规格：
盒
Rat Ceruloplasmin ELISA Kit
Product Description
Rat Ceruloplasmin ELISA Kit
In this assay the Ceruloplasmin present in samples reacts with the anti-Ceruloplasmin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CP in the test sample. The quantity of CP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
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