相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
4°C, sealed storage, away from moisture and light
- 英文名:
T668
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
115532-50-8
- 规格:
10 mM * 1 mL/5 mg/10 mg/50 mg/100 mg
| 规格: | 10 mM * 1 mL | 产品价格: | ¥551.0 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | ¥500.0 |
| 规格: | 10 mg | 产品价格: | ¥800.0 |
| 规格: | 50 mg | 产品价格: | ¥2800.0 |
| 规格: | 100 mg | 产品价格: | ¥4500.0 |
MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
TMRM Perchlorate
CAS No. : 115532-50-8
MCE 国际站:TMRM Perchlorate
产品活性:罗丹明类染料是一种膜渗透性的阳离子荧光探针,可对线粒体膜电位进行特异性识别,从而附着在线粒体中,并产生明亮荧光,在一定浓度下,罗丹明类染料对细胞具有低毒性,因此普遍用于检测动物细胞、植物细胞以及微生物中的线粒体。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: 1.Preparation of TMRM working solution
1.1Preparation of the stock solution
Dissolve 1 mg TMRM in 525 μL DMSO to obtain 5 mM of stock solution.
1.2Preparation of TMRM working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
Note: Please adjust the concentration of TMRM working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.
相关产品:MitoSOX Red | BODIPY 493/503 | Nile Red | D-Luciferin potassium | JC-1 | FITC | Propidium Iodide | CFDA-SE | FITC-Dextran (MW 4000) | Dihydroethidium | DiR | Calcein-AM | Thiazolyl Blue | 2-NBDG | DiI | Fluo-4 AM | TMRE | Cy5.5 | Indocyanine green | Acridine Orange hydrochloride | CY5 | BODIPY 581/591 C11 | FM4-64 | CY5-SE | PKH 26 | DiD perchlorate | Diphenylterazine
热门产品线:重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验、与配体耦联、与肽耦联、与人工合成的寡聚核苷酸耦联的探针,可用于免疫组化、荧光原位杂交、受体标记等 2)FITC (fluorescent isothiocyanate) BODIPY FL 503/512 3)异硫氰基荧光素 494/518 4)TRITC 四甲基异硫氰基罗丹明 544/572 BODIPY TMR 543/569 5)Texas Red 德州红 595/615 BODIPY TR592/618 6)PE 藻红蛋白 565
光原位杂交: 染色体基因定位 单细胞凝胶电泳 GFP的表达 游离Ca2+ 的分布与定量 定位、定量研究中常用荧光探针 1)Amine-Reactive Probes与抗体耦联、与配体耦联、与肽耦联、与人工合成的寡聚核苷酸耦联的探针,可用于免疫组化、荧光原位杂交、受体标记等 2)FITC (fluorescent isothiocyanate) BODIPY FL 503/512 3)异硫氰基荧光素 494/518 4)TRITC 四甲基异硫氰基罗丹明 544/572 BODIPY
技术资料暂无技术资料 索取技术资料


















