pUC8-Not（pUC8Not）

pUC8-Not（pUC8Not）

质粒名称: pUC8-Not
其他名称:
质粒大小（bp）: 2664
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: M18133
其他属性: Created by Moore, July 1995, under contract with NCBI. Polylinker of pUC8 modified. General purpose vector modified to include a NotI site to facilitate library construction and chromosome walking. Restriction digests of the clone give the following sizes (kb): PstI–2.7; BamHI–uncut; EcoRI–2.7. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JM101, E.coli. Related vectors: pUC8.
质粒图谱:

 

 

质粒序列:

1	gcgcccaata	cgcaaaccgc	ctctccccgc	gcgttggccg	attcattaat	50
51	gcagctggca	cgacaggttt	cccgactgga	aagcgggcag	tgagcgcaac	100
101	gcaattaatg	tgagttagct	cactcattag	gcaccccagg	ctttacactt	150
151	tatgcttccg	gctcgtatgt	tgtgtggaat	tgtgagcgga	taacaatttc	200
201	acacaggaaa	cagctatgac	catgattacg	aattcccgcg	gccgctcgac	250
251	ctgcagccaa	gcttggcact	ggccgtcgtt	ttacaacgtc	gtgactggga	300
301	aaaccctggc	gttacccaac	ttaatcgcct	tgcagcacat	ccccctttcg	350
351	ccagctggcg	taatagcgaa	gaggcccgca	ccgatcgccc	ttcccaacag	400
401	ttgcgcagcc	tgaatggcga	atggcgcctg	atgcggtatt	ttctccttac	450
451	gcatctgtgc	ggtatttcac	accgcatatg	gtgcactctc	agtacaatct	500
501	gctctgatgc	cgcatagtta	agccagcccc	gacacccgcc	aacacccgct	550
551	gacgcgccct	gacgggcttg	tctgctcccg	gcatccgctt	acagacaagc	600
601	tgtgaccgtc	tccgggagct	gcatgtgtca	gaggttttca	ccgtcatcac	650
651	cgaaacgcgc	gagacgaaag	ggcctcgtga	tacgcctatt	tttataggtt	700
701	aatgtcatga	taataatggt	ttcttagacg	tcaggtggca	cttttcgggg	750
751	aaatgtgcgc	ggaaccccta	tttgtttatt	tttctaaata	cattcaaata	800
801	tgtatccgct	catgagacaa	taaccctgat	aaatgcttca	ataatattga	850
851	aaaaggaaga	gtatgagtat	tcaacatttc	cgtgtcgccc	ttattccctt	900
901	ttttgcggca	ttttgccttc	ctgtttttgc	tcacccagaa	acgctggtga	950
951	aagtaaaaga	tgctgaagat	cagttgggtg	cacgagtggg	ttacatcgaa	1000
1001	ctggatctca	acagcggtaa	gatccttgag	agttttcgcc	ccgaagaacg	1050
1051	ttttccaatg	atgagcactt	ttaaagttct	gctatgtggc	gcggtattat	1100
1101	cccgtattga	cgccgggcaa	gagcaactcg	gtcgccgcat	acactattct	1150
1151	cagaatgact	tggttgagta	ctcaccagtc	acagaaaagc	atcttacgga	1200
1201	tggcatgaca	gtaagagaat	tatgcagtgc	tgccataacc	atgagtgata	1250
1251	acactgcggc	caacttactt	ctgacaacga	tcggaggacc	gaaggagcta	1300
1301	accgcttttt	tgcacaacat	gggggatcat	gtaactcgcc	ttgatcgttg	1350
1351	ggaaccggag	ctgaatgaag	ccataccaaa	cgacgagcgt	gacaccacga	1400
1401	tgcctgtagc	aatggcaaca	acgttgcgca	aactattaac	tggcgaacta	1450
1451	cttactctag	cttcccggca	acaattaata	gactggatgg	aggcggataa	1500
1501	agttgcagga	ccacttctgc	gctcggccct	tccggctggc	tggtttattg	1550
1551	ctgataaatc	tggagccggt	gagcgtgggt	ctcgcggtat	cattgcagca	1600
1601	ctggggccag	atggtaagcc	ctcccgtatc	gtagttatct	acacgacggg	1650
1651	gagtcaggca	actatggatg	aacgaaatag	acagatcgct	gagataggtg	1700
1701	cctcactgat	taagcattgg	taactgtcag	accaagttta	ctcatatata	1750
1751	ctttagattg	atttaaaact	tcatttttaa	tttaaaagga	tctaggtgaa	1800
1801	gatccttttt	gataatctca	tgaccaaaat	cccttaacgt	gagttttcgt	1850
1851	tccactgagc	gtcagacccc	gtagaaaaga	tcaaaggatc	ttcttgagat	1900
1901	cctttttttc	tgcgcgtaat	ctgctgcttg	caaacaaaaa	aaccaccgct	1950
1951	accagcggtg	gtttgtttgc	cggatcaaga	gctaccaact	ctttttccga	2000
2001	aggtaactgg	cttcagcaga	gcgcagatac	caaatactgt	ccttctagtg	2050
2051	tagccgtagt	taggccacca	cttcaagaac	tctgtagcac	cgcctacata	2100
2101	cctcgctctg	ctaatcctgt	taccagtggc	tgctgccagt	ggcgataagt	2150
2151	cgtgtcttac	cgggttggac	tcaagacgat	agttaccgga	taaggcgcag	2200
2201	cggtcgggct	gaacgggggg	ttcgtgcaca	cagcccagct	tggagcgaac	2250
2251	gacctacacc	gaactgagat	acctacagcg	tgagctatga	gaaagcgcca	2300
2301	cgcttcccga	agggagaaag	gcggacaggt	atccggtaag	cggcagggtc	2350
2351	ggaacaggag	agcgcacgag	ggagcttcca	gggggaaacg	cctggtatct	2400
2401	ttatagtcct	gtcgggtttc	gccacctctg	acttgagcgt	cgatttttgt	2450
2451	gatgctcgtc	aggggggcgg	agcctatgga	aaaacgccag	caacgcggcc	2500
2501	tttttacggt	tcctggcctt	ttgctggcct	tttgctcaca	tgttctttcc	2550
2551	tgcgttatcc	cctgattctg	tggataaccg	tattaccgcc	tttgagtgag	2600
2601	ctgataccgc	tcgccgcagc	cgaacgaccg	agcgcagcga	gtcagtgagc	2650
2651	gaggaagcgg	aaga	2664