Mammalian gene targeting vector designed for replacement or insertional recombination. Suitable for hit-and-run strategies. Inserts must include the promoter and 5' coding sequences of an expressed gene ligated in frame with neo. The order of the major features of the plasmid is: NotI/MSC/SmaI – promoterless neoR –> – LEU2 –> – SalI/MCS/NotI – ampR/pMB1 ori. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: A.J.Brookes Hosts: vertebrate cells, E.coli.