氨基末端激酶1|2|3免疫荧光

英文名称JNK1+JNK2+JNK3
中文名称氨基末端激酶1/2/3抗体
别    名JNK1 + JNK2 + JNK3; JNK1/2/3; JNK1+2+3; JNK1 + JNK2 + JNK3; MAPK10; c Jun N terminal kinase 1; c Jun N terminal kinase 2; c Jun N terminal kinase 3; JNK; JNK1; JNK2; JNK2ALPHA; JNK2BETA; JNK3; MAPK8; MAPK9; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9; SAPK(beta); Stress activated protein kinase JNK1; Stress activated protein kinase JNK2; Stress activated protein kinase JNK3; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA.  
文献引用

研究领域细胞生物  细胞凋亡  糖尿病  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量42-47kDa
细胞定位细胞核 细胞浆 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human JNK1/2/3:151-250/384 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
JNK1(MAPK8) is a member of the MAP kinase family. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7.
JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.
JNK3 is a neuron-specific form of c-Jun N-terminal kinases. Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways of neuronal apoptosis.
The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity.


SWISS:
Q61831

Gene ID:
5599

Database links:
Entrez Gene: 5599 Human
Entrez Gene: 26419 Mouse
Entrez Gene: 116554 Rat
Omim: 601158 Human JNK1
SwissProt: P45983 Human JNK1
Unigene: 138211 Human JNK1
Unigene: 21495 Mouse JNK1
Unigene: 4090 Rat JNK1
Entrez Gene: 5601 Human JNK2
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 

产品图片

Sample:  Kidney (Mouse) Lysate at 40 ug Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42-47 kD Observed band size:42-52 kD Sample:  Hela(Human) CellLysate at 30 ug Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42-47 kD Observed band size:42-52 kD Sample:  K562 (Human) Lysate at 30 ug Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42-47 kD Observed band size:42-52 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-JNK1/2/3 Polyclonal Antibody, Unconjugated(bs-2592R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-JNK1+JNK2+JNK3 antibody (bs-9587R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.