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氨基末端激酶1|2|3免疫荧光
抗体KLH conjugated
IgG
冻干粉
零下20度保存
多克隆
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人，大小鼠等多抗
Rabbit
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
1mg/ml
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JNK1+JNK2+JNK3
JNK1+JNK2+JNK3
100ul
| 产品图片 |  Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42-47 kD Observed band size:42-52 kD  Sample: Hela(Human) CellLysate at 30 ug Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42-47 kD Observed band size:42-52 kD  Sample: K562 (Human) Lysate at 30 ug Primary: Anti-JNK1+JNK2+JNK3 (bs-2592R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42-47 kD Observed band size:42-52 kD  Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JNK1+JNK2+JNK3) Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-JNK1/2/3 Polyclonal Antibody, Unconjugated(bs-2592R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-JNK1+JNK2+JNK3 antibody (bs-9587R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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