Human Apolipoprotein H ELISA Kit is designed for detection of human Apo H in plasma, serum, urine, saliva, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Apo H in less than 4 hours. A polyclonal antibody specific for human Apo H has been pre-coated onto a 96-well microplate with removable strips. ApoH in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for ApoH, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.