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- CYT-251
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
Hepatocyte Growth Factor Human Recombinant, CHO
- 库存：
大量
- 供应商：
上海沪震实业有限公司
- 规格：
2μg/10μg/1mg
CATALOGUE NUMBER
CYT-251
SYNONYMS
Scatter Factor (SF), Hepatopoietin (HPTA), HGF.
INTRODUCTION
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.
DESCRIPTION
Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of approximately 75kDa.
The HGF is purified by proprietary chromatographic techniques.
SOURCE
Chinese Hamster Ovarian Cells.PHYSICAL APPEARANCE
Sterile Filtered White lyophilized (freeze-dried) powder.
FORMULATION
The protein was lyophilized from a concentrated (292µg/ml) solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4.
SOLUBILITY
It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
STABILITY
Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
PURITY
Greater than 97.0% as determined by SDS-PAGE.
BIOLOGICAL ACTIVITY
The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.
USAGE
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文献和实验up 300 ml of CHO medium to 37 deg.C and add cytochalasin B (150 ul of 10 mg/ml) and nocodazole (300 ul of 10 mg/ml) Make sure all buffers are in coldroom, there is rocker in the coldroom and there is a good aspirator in the coldroom. Hook up a sawed
up 300 ml of CHO medium to 37 deg.C and add cytochalasin B (150 ul of 10 mg/ml) and nocodazole (300 ul of 10 mg/ml) Make sure all buffers are in coldroom, there is rocker in the coldroom and there is a good aspirator in the coldroom. Hook up a sawed
for sialic acid transfer has not yet succeeded in providing a human-like pattern of glycoforms to protein drugs. To date, there is a need for engineering animal cells and get highly sialylated products that resemble as closely as possible to human proteins
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