pIA2 质粒名称: pIA2
其他名称:
质粒大小(bp): 9300
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
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GenBank号:
其他属性: YE-type shuttle vector permitting FLP-driven high copy number. (personal communication) In S. cerevisiae, requires a host expressing FLP such as YPH485 or YPH494, or a host modified by pFV17 (ATCC 77170). (personal communication) When propagated in a strain carrying an integrated copy of GAL10-FLP, the copy number is 10-20 without induction and 200-400 when induced by galactose. The order of the major features in this plasmid is:REP3 – D – REP1 – REP2 – TRP1 – MCS (NotI – BamHI/NotI) – kanR. Constructed by inserting a BalI/EcoRV fragment of the 2 micron circle (interrupting FLP) into the SmaI site of pHSS6 which had been modified by the addition of a TRP1 gene. Restriction digests of the clone give the following sizes (kb): HindIII–4.8,2.2,1.4, 1.2; BamHI–9.6; NotI–9.6; ClaI–9.6. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH485, Saccharomyces cerevisiae YPH494.