- ¥1 - 1880
- LMAI Bio
- 中国/上海
- LM2966
- 2025年12月11日
10 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-49b(+)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pET-49b(+)
质粒名称: pET-49b(+)
其他名称: pET49b, pET 49b
质粒大小（bp）: 5926
抗性: Kanamycin
质粒类型: Bacterial Expression（大肠杆菌蛋白表达）
是否病毒: 非病毒
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-GST, N-His, N-HRV 3C, C-S, C-Thrombin
5′ 测序引物及序列:T7: 5′-TAATACGACTCACTATAGGG-3′; pGEX 5′: 5′-GGGCTGGCAAGCCACGTTTGGTG-3′
3′ 测序引物及序列: T7t: 5′-TGCTAGTTATTGCTCAGCGG-3′
GenBank号:
其他属性: Same as pET47 but also has Nterm GST Tag; contains HRV 3C Protease cleavage site for fusion tag removal at low temperatures; Cterm thrombin cleavage site.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 120 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| S15 | 212 | 168 | |
| 6xHIS | 359 | 342 | |
| GST (variant) | 1088 | 438 | |
| T7_transl_en_RBS | 1113 | 1097 | |
| lacO | 1158 | 1131 | |
| T7_promoter | 1176 | 1158 | |
| tet (300 – 563) | 1212 | 1475 | |
| pBRrevBam_primer | 1283 | 1264 | |
| lacI | 1558 | 2649 | |
| ROP | 3221 | 3412 | |
| pGEX_3_primer | 3428 | 3406 | |
| pBR322_origin | 4446 | 3827 | |
| KanR2 | 4552 | 5367 | |
| f1_origin | 5898 | 5592 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 1088 | 141 | |
| ORF frame 3 | 1089 | 1817 | |
| ORF frame 1 | 1690 | 2649 | |
| ORF frame 1 | 4552 | 5367 |
| Enzyme Name | Cut |
|---|---|
| PacI | 141 |
| XhoI | 147 |
| SacI | 238 |
| NotI | 242 |
| EagI | 242 |
| HindIII | 249 |
| SalI | 255 |
| AatII | 266 |
| AscI | 270 |
| StuI | 280 |
| EcoRI | 289 |
| BamHI | 295 |
| KpnI | 306 |
| XmaI | 306 |
| SmaI | 308 |
| SacII | 338 |
| SpeI | 417 |
| MscI | 880 |
| NdeI | 1087 |
| XbaI | 1125 |
| ApaI | 2128 |
| HpaI | 2423 |
| NruI | 4640 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgtttagcag | cctaggttaa | ttaagcctcg | 150 |
| 151 | agagcagcag | aagtagagct | gtccatgtgc | tggcgttcga | atttagcagc | 200 |
| 201 | agcggtttct | ttactaccgc | gtggcaccag | agcgagctct | gcggccgcaa | 250 |
| 251 | gcttgtcgac | ggacgtcggg | cgcgccaagg | cctgtacaga | attcggatcc | 300 |
| 301 | tggtacccgg | gtccctgaaa | gaggacttca | agagccgcgg | agtgatggtg | 350 |
| 351 | atggtgatga | ccagaaccac | tagatggagt | aggagtagga | ggattgttat | 400 |
| 401 | tagaaccgcc | accaccacta | gttgaaccat | ccgattttgg | aggatggtcg | 450 |
| 451 | ccaccaccaa | acgtggcttg | ccagccctgc | aaaggccatg | ctatatactt | 500 |
| 501 | gctggatttc | aagtacttat | caatttgtgg | gatagcttca | atacgttttt | 550 |
| 551 | taaaacaaac | taattttggg | aacgcatcca | ggcacattgg | gtccatgtat | 600 |
| 601 | aaaacaacat | caagagcgtc | atacaacatg | aagtcaggat | gggttacatg | 650 |
| 651 | atcaccattt | aaatatgttt | tatgacataa | acgatcttcg | aacattttca | 700 |
| 701 | gcatttcagg | tagcttgcta | agaaaatcaa | ctttgagagt | ttcaaagtct | 750 |
| 751 | ttactatatg | caattctcga | aacaccgtat | ctaatatcca | aaaccgctcc | 800 |
| 801 | ttcaagcatt | gaaatctctg | cacgctcttt | tggacaacca | cccaacatgt | 850 |
| 851 | tgtgcttgtc | agctatataa | cgtatgatgg | ccatagactg | tgttaattta | 900 |
| 901 | acatcaccat | caatataata | aggaagattg | ggaaactcca | aacccaattc | 950 |
| 951 | aaactttttg | tttcgccatt | tatcaccttc | atcgcgctca | tacaaatgct | 1000 |
| 1001 | cttcatattt | ttcttcaaga | tattccaaaa | gaagtcgagt | gggttgcaca | 1050 |
| 1051 | aggcccttaa | ttttccaata | acctagtata | ggggacatat | gtatatctcc | 1100 |
| 1101 | ttcttaaagt | taaacaaaat | tatttctaga | ggggaattgt | tatccgctca | 1150 |
| 1151 | caattcccct | atagtgagtc | gtattaattt | cgcgggatcg | agatcgatct | 1200 |
| 1201 | cgatcctcta | cgccggacgc | atcgtggccg | gcatcaccgg | cgccacaggt | 1250 |
| 1251 | gcggttgctg | gcgcctatat | cgccgacatc | accgatgggg | aagatcgggc | 1300 |
| 1301 | tcgccacttc | gggctcatga | gcgcttgttt | cggcgtgggt | atggtggcag | 1350 |
| 1351 | gccccgtggc | cgggggactg | ttgggcgcca | tctccttgca | tgcaccattc | 1400 |
| 1401 | cttgcggcgg | cggtgctcaa | cggcctcaac | ctactactgg | gctgcttcct | 1450 |
| 1451 | aatgcaggag | tcgcataagg | gagagcgtcg | agatcccgga | caccatcgaa | 1500 |
| 1501 | tggcgcaaaa | cctttcgcgg | tatggcatga | tagcgcccgg | aagagagtca | 1550 |
| 1551 | attcagggtg | gtgaatgtga | aaccagtaac | gttatacgat | gtcgcagagt | 1600 |
| 1601 | atgccggtgt | ctcttatcag | accgtttccc | gcgtggtgaa | ccaggccagc | 1650 |
| 1651 | cacgtttctg | cgaaaacgcg | ggaaaaagtg | gaagcggcga | tggcggagct | 1700 |
| 1701 | gaattacatt | cccaaccgcg | tggcacaaca | actggcgggc | aaacagtcgt | 1750 |
| 1751 | tgctgattgg | cgttgccacc | tccagtctgg | ccctgcacgc | gccgtcgcaa | 1800 |
| 1801 | attgtcgcgg | cgattaaatc | tcgcgccgat | caactgggtg | ccagcgtggt | 1850 |
| 1851 | ggtgtcgatg | gtagaacgaa | gcggcgtcga | agcctgtaaa | gcggcggtgc | 1900 |
| 1901 | acaatcttct | cgcgcaacgc | gtcagtgggc | tgatcattaa | ctatccgctg | 1950 |
| 1951 | gatgaccagg | atgccattgc | tgtggaagct | gcctgcacta | atgttccggc | 2000 |
| 2001 | gttatttctt | gatgtctctg | accagacacc | catcaacagt | attattttct | 2050 |
| 2051 | cccatgaaga | cggtacgcga | ctgggcgtgg | agcatctggt | cgcattgggt | 2100 |
| 2101 | caccagcaaa | tcgcgctgtt | agcgggccca | ttaagttctg | tctcggcgcg | 2150 |
| 2151 | tctgcgtctg | gctggctggc | ataaatatct | cactcgcaat | caaattcagc | 2200 |
| 2201 | cgatagcgga | acgggaaggc | gactggagtg | ccatgtccgg | ttttcaacaa | 2250 |
| 2251 | accatgcaaa | tgctgaatga | gggcatcgtt | cccactgcga | tgctggttgc | 2300 |
| 2301 | caacgatcag | atggcgctgg | gcgcaatgcg | cgccattacc | gagtccgggc | 2350 |
| 2351 | tgcgcgttgg | tgcggacatc | tcggtagtgg | gatacgacga | taccgaagac | 2400 |
| 2401 | agctcatgtt | atatcccgcc | gttaaccacc | atcaaacagg | attttcgcct | 2450 |
| 2451 | gctggggcaa | accagcgtgg | accgcttgct | gcaactctct | cagggccagg | 2500 |
| 2501 | cggtgaaggg | caatcagctg | ttgcccgtct | cactggtgaa | aagaaaaacc | 2550 |
| 2551 | accctggcgc | ccaatacgca | aaccgcctct | ccccgcgcgt | tggccgattc | 2600 |
| 2601 | attaatgcag | ctggcacgac | aggtttcccg | actggaaagc | gggcagtgag | 2650 |
| 2651 | cgcaacgcaa | ttaatgtaag | ttagctcact | cattaggcac | cgggatctcg | 2700 |
| 2701 | accgatgccc | ttgagagcct | tcaacccagt | cagctccttc | cggtgggcgc | 2750 |
| 2751 | ggggcatgac | tagcatgatc | gtgctcctgt | cgttgaggac | ccggctaggc | 2800 |
| 2801 | tggcggggtt | gccttactgg | ttagcagaat | gaatcaccga | tacgcgagcg | 2850 |
| 2851 | aacgtgaagc | gactgctgct | gcaaaacgtc | tgcgacctga | gcaacaacat | 2900 |
| 2901 | gaatggtctt | cggtttccgt | gtttcgtaaa | gtctggaaac | gcggaagtca | 2950 |
| 2951 | gcgccctgca | ccattatgtt | ccggatctgc | atcgcaggat | gctgctggct | 3000 |
| 3001 | accctgtgga | acacctacat | ctgtattaac | gaagcgctgg | cattgaccct | 3050 |
| 3051 | gagtgatttt | tctctggtcc | cgccgcatcc | ataccgccag | ttgtttaccc | 3100 |
| 3101 | tcacaacgtt | ccagtaaccg | ggcatgttca | tcatcagtaa | cccgtatcgt | 3150 |
| 3151 | gagcatcctc | tctcgtttca | tcggtatcat | tacccccatg | aacagaaatc | 3200 |
| 3201 | ccccttacac | ggaggcatca | gtgaccaaac | aggaaaaaac | cgcccttaac | 3250 |
| 3251 | atggcccgct | ttatcagaag | ccagacatta | acgcttctgg | agaaactcaa | 3300 |
| 3301 | cgagctggac | gcggatgaac | aggcagacat | ctgtgaatcg | cttcacgacc | 3350 |
| 3351 | acgctgatga | gctttaccgc | agctgcctcg | cgcgtttcgg | tgatgacggt | 3400 |
| 3401 | gaaaacctct | gacacatgca | gctcccggag | acggtcacag | cttgtctgta | 3450 |
| 3451 | agcggatgcc | gggagcagac | aagcccgtca | gggcgcgtca | gcgggtgttg | 3500 |
| 3501 | gcgggtgtcg | gggcgcagcc | atgacccagt | cacgtagcga | tagcggagtg | 3550 |
| 3551 | tatactggct | taactatgcg | gcatcagagc | agattgtact | gagagtgcac | 3600 |
| 3601 | catatatgcg | gtgtgaaata | ccgcacagat | gcgtaaggag | aaaataccgc | 3650 |
| 3651 | atcaggcgct | cttccgcttc | ctcgctcact | gactcgctgc | gctcggtcgt | 3700 |
| 3701 | tcggctgcgg | cgagcggtat | cagctcactc | aaaggcggta | atacggttat | 3750 |
| 3751 | ccacagaatc | aggggataac | gcaggaaaga | acatgtgagc | aaaaggccag | 3800 |
| 3801 | caaaaggcca | ggaaccgtaa | aaaggccgcg | ttgctggcgt | ttttccatag | 3850 |
| 3851 | gctccgcccc | cctgacgagc | atcacaaaaa | tcgacgctca | agtcagaggt | 3900 |
| 3901 | ggcgaaaccc | gacaggacta | taaagatacc | aggcgtttcc | ccctggaagc | 3950 |
| 3951 | tccctcgtgc | gctctcctgt | tccgaccctg | ccgcttaccg | gatacctgtc | 4000 |
| 4001 | cgcctttctc | ccttcgggaa | gcgtggcgct | ttctcatagc | tcacgctgta | 4050 |
| 4051 | ggtatctcag | ttcggtgtag | gtcgttcgct | ccaagctggg | ctgtgtgcac | 4100 |
| 4101 | gaaccccccg | ttcagcccga | ccgctgcgcc | ttatccggta | actatcgtct | 4150 |
| 4151 | tgagtccaac | ccggtaagac | acgacttatc | gccactggca | gcagccactg | 4200 |
| 4201 | gtaacaggat | tagcagagcg | aggtatgtag | gcggtgctac | agagttcttg | 4250 |
| 4251 | aagtggtggc | ctaactacgg | ctacactaga | aggacagtat | ttggtatctg | 4300 |
| 4301 | cgctctgctg | aagccagtta | ccttcggaaa | aagagttggt | agctcttgat | 4350 |
| 4351 | ccggcaaaca | aaccaccgct | ggtagcggtg | gtttttttgt | ttgcaagcag | 4400 |
| 4401 | cagattacgc | gcagaaaaaa | aggatctcaa | gaagatcctt | tgatcttttc | 4450 |
| 4451 | tacggggtct | gacgctcagt | ggaacgaaaa | ctcacgttaa | gggattttgg | 4500 |
| 4501 | tcatgaacaa | taaaactgtc | tgcttacata | aacagtaata | caaggggtgt | 4550 |
| 4551 | tatgagccat | attcaacggg | aaacgtcttg | ctctaggccg | cgattaaatt | 4600 |
| 4601 | ccaacatgga | tgctgattta | tatgggtata | aatgggctcg | cgataatgtc | 4650 |
| 4651 | gggcaatcag | gtgcgacaat | ctatcgattg | tatgggaagc | ccgatgcgcc | 4700 |
| 4701 | agagttgttt | ctgaaacatg | gcaaaggtag | cgttgccaat | gatgttacag | 4750 |
| 4751 | atgagatggt | cagactaaac | tggctgacgg | aatttatgcc | tcttccgacc | 4800 |
| 4801 | atcaagcatt | ttatccgtac | tcctgatgat | gcatggttac | tcaccactgc | 4850 |
| 4851 | gatccccggc | aaaacagcat | tccaggtatt | agaagaatat | cctgattcag | 4900 |
| 4901 | gtgaaaatat | tgttgatgcg | ctggcagtgt | tcctgcgccg | gttgcattcg | 4950 |
| 4951 | attcctgttt | gtaattgtcc | ttttaacagt | gatcgcgtat | ttcgtctcgc | 5000 |
| 5001 | tcaggcgcaa | tcacgaatga | ataacggttt | ggttgatgcg | agtgattttg | 5050 |
| 5051 | atgacgagcg | taatggctgg | cctgttgaac | aagtctggaa | agaaatgcat | 5100 |
| 5101 | aaacttttgc | cattctcacc | ggattcagtc | gtcactcatg | gtgatttctc | 5150 |
| 5151 | acttgataac | cttatttttg | acgaggggaa | attaataggt | tgtattgatg | 5200 |
| 5201 | ttggacgagt | cggaatcgca | gaccgatacc | aggatcttgc | catcctatgg | 5250 |
| 5251 | aactgcctcg | gtgagttttc | tccttcatta | cagaaacggc | tttttcaaaa | 5300 |
| 5301 | atatggtatt | gataatcctg | atatgaataa | attgcagttt | catttgatgc | 5350 |
| 5351 | tcgatgagtt | tttctaagaa | ttaattcatg | agcggataca | tatttgaatg | 5400 |
| 5401 | tatttagaaa | aataaacaaa | taggggttcc | gcgcacattt | ccccgaaaag | 5450 |
| 5451 | tgccacctga | aattgtaaac | gttaatattt | tgttaaaatt | cgcgttaaat | 5500 |
| 5501 | ttttgttaaa | tcagctcatt | ttttaaccaa | taggccgaaa | tcggcaaaat | 5550 |
| 5551 | cccttataaa | tcaaaagaat | agaccgagat | agggttgagt | gttgttccag | 5600 |
| 5601 | tttggaacaa | gagtccacta | ttaaagaacg | tggactccaa | cgtcaaaggg | 5650 |
| 5651 | cgaaaaaccg | tctatcaggg | cgatggccca | ctacgtgaac | catcacccta | 5700 |
| 5701 | atcaagtttt | ttggggtcga | ggtgccgtaa | agcactaaat | cggaacccta | 5750 |
| 5751 | aagggagccc | ccgatttaga | gcttgacggg | gaaagccggc | gaacgtggcg | 5800 |
| 5801 | agaaaggaag | ggaagaaagc | gaaaggagcg | ggcgctaggg | cgctggcaag | 5850 |
| 5851 | tgtagcggtc | acgctgcgcg | taaccaccac | acccgccgcg | cttaatgcgc | 5900 |
| 5901 | cgctacaggg | cgcgtcccat | tcgcca | 5926 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
, either alone or in combination with chemo- or radiotherapy at the more advanced stages. However, many patients present with metastatic disease at the time of diagnosis. Both computed tomography (CT) and positron emission tomography (PET) using fluorodeoxyglucose (FDG) play
什么是PET 树脂 ---PET是英文Polyethylene terephthalate的缩写，PET的中文意思是聚对苯二甲酸乙二醇酯。PET是以石油中的对苯二甲酸和乙二醇为原料，在高温高真空条件下进行化学反应而形成的树脂之一。PET瓶具有综合性能优良的特点，在饮料行业被广泛使用。 核医学 ---PET是英文Positron Emission Tomography的缩写，PET的中文意思是正电子发射计算机断层扫描。PET是核医学发展的一项新技术。 PET瓶片－－
PET and PET/CT in the Management of Thyroid Cancer
The introduction of PET(-CT) has brought about a major paradigm shift in the management of thyroid carcinoma, especially from the diagnostic standpoint. From the viewpoint of patient management, the areas where it has made significant impact
技术资料暂无技术资料 索取技术资料
pET-49b(+)
¥1 - 1880
