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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-25b(+)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pET-25b(+)
质粒名称: pET-25b(+)
其他名称: pET25b, pet 25b
质粒大小（bp）: 5547
抗性: Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-pelB, C-HSV, C-His
5’测序引物:T7
5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: PelB sequence for periplasmic localization; same as pET27b but ampR
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| T7_transl_en_RBS | 368 | 352 | |
| lacO | 413 | 386 | |
| T7_promoter | 431 | 413 | |
| tet (300 – 563) | 463 | 726 | |
| pBRrevBam_primer | 534 | 515 | |
| lacI | 809 | 1900 | |
| tet (576 – 851) | 1959 | 2234 | |
| ROP | 2709 | 2900 | |
| pGEX_3_primer | 2916 | 2894 | |
| pBR322_origin | 3934 | 3315 | |
| Ampicillin | 4949 | 4089 | |
| AmpR_promoter | 5019 | 4991 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 568 | 1068 | |
| ORF frame 2 | 941 | 1900 | |
| ORF frame 2 | 4949 | 4089 |
| Enzyme Name | Cut |
|---|---|
| NheI | 197 |
| XhoI | 212 |
| NotI | 220 |
| EagI | 220 |
| HindIII | 227 |
| SalI | 233 |
| SacI | 244 |
| EcoRI | 246 |
| BamHI | 252 |
| NcoI | 274 |
| MscI | 279 |
| NdeI | 342 |
| XbaI | 380 |
| BglII | 446 |
| BclI | 1182 |
| ApaI | 1379 |
| HpaI | 1674 |
| PstI | 4407 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gacatcctcg | gggtcttccg | gggcgagttc | tggctggcta | 200 |
| 201 | gcccgtttga | tctcgagtgc | ggccgcaagc | ttgtcgacgg | agctcgaatt | 250 |
| 251 | cggatccgaa | ttaattccga | tatccatggc | catcgccggc | tgggcagcga | 300 |
| 301 | ggagcagcag | accagcagca | gcggtcggca | gcaggtattt | catatgtata | 350 |
| 351 | tctccttctt | aaagttaaac | aaaattattt | ctagagggga | attgttatcc | 400 |
| 401 | gctcacaatt | cccctatagt | gagtcgtatt | aatttcgcgg | gatcgagatc | 450 |
| 451 | tcgatcctct | acgccggacg | catcgtggcc | ggcatcaccg | gcgccacagg | 500 |
| 501 | tgcggttgct | ggcgcctata | tcgccgacat | caccgatggg | gaagatcggg | 550 |
| 551 | ctcgccactt | cgggctcatg | agcgcttgtt | tcggcgtggg | tatggtggca | 600 |
| 601 | ggccccgtgg | ccgggggact | gttgggcgcc | atctccttgc | atgcaccatt | 650 |
| 651 | ccttgcggcg | gcggtgctca | acggcctcaa | cctactactg | ggctgcttcc | 700 |
| 701 | taatgcagga | gtcgcataag | ggagagcgtc | gagatcccgg | acaccatcga | 750 |
| 751 | atggcgcaaa | acctttcgcg | gtatggcatg | atagcgcccg | gaagagagtc | 800 |
| 801 | aattcagggt | ggtgaatgtg | aaaccagtaa | cgttatacga | tgtcgcagag | 850 |
| 851 | tatgccggtg | tctcttatca | gaccgtttcc | cgcgtggtga | accaggccag | 900 |
| 901 | ccacgtttct | gcgaaaacgc | gggaaaaagt | ggaagcggcg | atggcggagc | 950 |
| 951 | tgaattacat | tcccaaccgc | gtggcacaac | aactggcggg | caaacagtcg | 1000 |
| 1001 | ttgctgattg | gcgttgccac | ctccagtctg | gccctgcacg | cgccgtcgca | 1050 |
| 1051 | aattgtcgcg | gcgattaaat | ctcgcgccga | tcaactgggt | gccagcgtgg | 1100 |
| 1101 | tggtgtcgat | ggtagaacga | agcggcgtcg | aagcctgtaa | agcggcggtg | 1150 |
| 1151 | cacaatcttc | tcgcgcaacg | cgtcagtggg | ctgatcatta | actatccgct | 1200 |
| 1201 | ggatgaccag | gatgccattg | ctgtggaagc | tgcctgcact | aatgttccgg | 1250 |
| 1251 | cgttatttct | tgatgtctct | gaccagacac | ccatcaacag | tattattttc | 1300 |
| 1301 | tcccatgaag | acggtacgcg | actgggcgtg | gagcatctgg | tcgcattggg | 1350 |
| 1351 | tcaccagcaa | atcgcgctgt | tagcgggccc | attaagttct | gtctcggcgc | 1400 |
| 1401 | gtctgcgtct | ggctggctgg | cataaatatc | tcactcgcaa | tcaaattcag | 1450 |
| 1451 | ccgatagcgg | aacgggaagg | cgactggagt | gccatgtccg | gttttcaaca | 1500 |
| 1501 | aaccatgcaa | atgctgaatg | agggcatcgt | tcccactgcg | atgctggttg | 1550 |
| 1551 | ccaacgatca | gatggcgctg | ggcgcaatgc | gcgccattac | cgagtccggg | 1600 |
| 1601 | ctgcgcgttg | gtgcggatat | ctcggtagtg | ggatacgacg | ataccgaaga | 1650 |
| 1651 | cagctcatgt | tatatcccgc | cgttaaccac | catcaaacag | gattttcgcc | 1700 |
| 1701 | tgctggggca | aaccagcgtg | gaccgcttgc | tgcaactctc | tcagggccag | 1750 |
| 1751 | gcggtgaagg | gcaatcagct | gttgcccgtc | tcactggtga | aaagaaaaac | 1800 |
| 1801 | caccctggcg | cccaatacgc | aaaccgcctc | tccccgcgcg | ttggccgatt | 1850 |
| 1851 | cattaatgca | gctggcacga | caggtttccc | gactggaaag | cgggcagtga | 1900 |
| 1901 | gcgcaacgca | attaatgtaa | gttagctcac | tcattaggca | ccgggatctc | 1950 |
| 1951 | gaccgatgcc | cttgagagcc | ttcaacccag | tcagctcctt | ccggtgggcg | 2000 |
| 2001 | cggggcatga | ctatcgtcgc | cgcacttatg | actgtcttct | ttatcatgca | 2050 |
| 2051 | actcgtagga | caggtgccgg | cagcgctctg | ggtcattttc | ggcgaggacc | 2100 |
| 2101 | gctttcgctg | gagcgcgacg | atgatcggcc | tgtcgcttgc | ggtattcgga | 2150 |
| 2151 | atcttgcacg | ccctcgctca | agccttcgtc | actggtcccg | ccaccaaacg | 2200 |
| 2201 | tttcggcgag | aagcaggcca | ttatcgccgg | catggcggcc | ccacgggtgc | 2250 |
| 2251 | gcatgatcgt | gctcctgtcg | ttgaggaccc | ggctaggctg | gcggggttgc | 2300 |
| 2301 | cttactggtt | agcagaatga | atcaccgata | cgcgagcgaa | cgtgaagcga | 2350 |
| 2351 | ctgctgctgc | aaaacgtctg | cgacctgagc | aacaacatga | atggtcttcg | 2400 |
| 2401 | gtttccgtgt | ttcgtaaagt | ctggaaacgc | ggaagtcagc | gccctgcacc | 2450 |
| 2451 | attatgttcc | ggatctgcat | cgcaggatgc | tgctggctac | cctgtggaac | 2500 |
| 2501 | acctacatct | gtattaacga | agcgctggca | ttgaccctga | gtgatttttc | 2550 |
| 2551 | tctggtcccg | ccgcatccat | accgccagtt | gtttaccctc | acaacgttcc | 2600 |
| 2601 | agtaaccggg | catgttcatc | atcagtaacc | cgtatcgtga | gcatcctctc | 2650 |
| 2651 | tcgtttcatc | ggtatcatta | cccccatgaa | cagaaatccc | ccttacacgg | 2700 |
| 2701 | aggcatcagt | gaccaaacag | gaaaaaaccg | cccttaacat | ggcccgcttt | 2750 |
| 2751 | atcagaagcc | agacattaac | gcttctggag | aaactcaacg | agctggacgc | 2800 |
| 2801 | ggatgaacag | gcagacatct | gtgaatcgct | tcacgaccac | gctgatgagc | 2850 |
| 2851 | tttaccgcag | ctgcctcgcg | cgtttcggtg | atgacggtga | aaacctctga | 2900 |
| 2901 | cacatgcagc | tcccggagac | ggtcacagct | tgtctgtaag | cggatgccgg | 2950 |
| 2951 | gagcagacaa | gcccgtcagg | gcgcgtcagc | gggtgttggc | gggtgtcggg | 3000 |
| 3001 | gcgcagccat | gacccagtca | cgtagcgata | gcggagtgta | tactggctta | 3050 |
| 3051 | actatgcggc | atcagagcag | attgtactga | gagtgcacca | tatatgcggt | 3100 |
| 3101 | gtgaaatacc | gcacagatgc | gtaaggagaa | aataccgcat | caggcgctct | 3150 |
| 3151 | tccgcttcct | cgctcactga | ctcgctgcgc | tcggtcgttc | ggctgcggcg | 3200 |
| 3201 | agcggtatca | gctcactcaa | aggcggtaat | acggttatcc | acagaatcag | 3250 |
| 3251 | gggataacgc | aggaaagaac | atgtgagcaa | aaggccagca | aaaggccagg | 3300 |
| 3301 | aaccgtaaaa | aggccgcgtt | gctggcgttt | ttccataggc | tccgcccccc | 3350 |
| 3351 | tgacgagcat | cacaaaaatc | gacgctcaag | tcagaggtgg | cgaaacccga | 3400 |
| 3401 | caggactata | aagataccag | gcgtttcccc | ctggaagctc | cctcgtgcgc | 3450 |
| 3451 | tctcctgttc | cgaccctgcc | gcttaccgga | tacctgtccg | cctttctccc | 3500 |
| 3501 | ttcgggaagc | gtggcgcttt | ctcatagctc | acgctgtagg | tatctcagtt | 3550 |
| 3551 | cggtgtaggt | cgttcgctcc | aagctgggct | gtgtgcacga | accccccgtt | 3600 |
| 3601 | cagcccgacc | gctgcgcctt | atccggtaac | tatcgtcttg | agtccaaccc | 3650 |
| 3651 | ggtaagacac | gacttatcgc | cactggcagc | agccactggt | aacaggatta | 3700 |
| 3701 | gcagagcgag | gtatgtaggc | ggtgctacag | agttcttgaa | gtggtggcct | 3750 |
| 3751 | aactacggct | acactagaag | gacagtattt | ggtatctgcg | ctctgctgaa | 3800 |
| 3801 | gccagttacc | ttcggaaaaa | gagttggtag | ctcttgatcc | ggcaaacaaa | 3850 |
| 3851 | ccaccgctgg | tagcggtggt | ttttttgttt | gcaagcagca | gattacgcgc | 3900 |
| 3901 | agaaaaaaag | gatctcaaga | agatcctttg | atcttttcta | cggggtctga | 3950 |
| 3951 | cgctcagtgg | aacgaaaact | cacgttaagg | gattttggtc | atgagattat | 4000 |
| 4001 | caaaaaggat | cttcacctag | atccttttaa | attaaaaatg | aagttttaaa | 4050 |
| 4051 | tcaatctaaa | gtatatatga | gtaaacttgg | tctgacagtt | accaatgctt | 4100 |
| 4101 | aatcagtgag | gcacctatct | cagcgatctg | tctatttcgt | tcatccatag | 4150 |
| 4151 | ttgcctgact | ccccgtcgtg | tagataacta | cgatacggga | gggcttacca | 4200 |
| 4201 | tctggcccca | gtgctgcaat | gataccgcga | gacccacgct | caccggctcc | 4250 |
| 4251 | agatttatca | gcaataaacc | agccagccgg | aagggccgag | cgcagaagtg | 4300 |
| 4301 | gtcctgcaac | tttatccgcc | tccatccagt | ctattaattg | ttgccgggaa | 4350 |
| 4351 | gctagagtaa | gtagttcgcc | agttaatagt | ttgcgcaacg | ttgttgccat | 4400 |
| 4401 | tgctgcaggc | atcgtggtgt | cacgctcgtc | gtttggtatg | gcttcattca | 4450 |
| 4451 | gctccggttc | ccaacgatca | aggcgagtta | catgatcccc | catgttgtgc | 4500 |
| 4501 | aaaaaagcgg | ttagctcctt | cggtcctccg | atcgttgtca | gaagtaagtt | 4550 |
| 4551 | ggccgcagtg | ttatcactca | tggttatggc | agcactgcat | aattctctta | 4600 |
| 4601 | ctgtcatgcc | atccgtaaga | tgcttttctg | tgactggtga | gtactcaacc | 4650 |
| 4651 | aagtcattct | gagaatagtg | tatgcggcga | ccgagttgct | cttgcccggc | 4700 |
| 4701 | gtcaatacgg | gataataccg | cgccacatag | cagaacttta | aaagtgctca | 4750 |
| 4751 | tcattggaaa | acgttcttcg | gggcgaaaac | tctcaaggat | cttaccgctg | 4800 |
| 4801 | ttgagatcca | gttcgatgta | acccactcgt | gcacccaact | gatcttcagc | 4850 |
| 4851 | atcttttact | ttcaccagcg | tttctgggtg | agcaaaaaca | ggaaggcaaa | 4900 |
| 4901 | atgccgcaaa | aaagggaata | agggcgacac | ggaaatgttg | aatactcata | 4950 |
| 4951 | ctcttccttt | ttcaatatta | ttgaagcatt | tatcagggtt | attgtctcat | 5000 |
| 5001 | gagcggatac | atatttgaat | gtatttagaa | aaataaacaa | ataggggttc | 5050 |
| 5051 | cgcgcacatt | tccccgaaaa | gtgccacctg | aaattgtaaa | cgttaatatt | 5100 |
| 5101 | ttgttaaaat | tcgcgttaaa | tttttgttaa | atcagctcat | tttttaacca | 5150 |
| 5151 | ataggccgaa | atcggcaaaa | tcccttataa | atcaaaagaa | tagaccgaga | 5200 |
| 5201 | tagggttgag | tgttgttcca | gtttggaaca | agagtccact | attaaagaac | 5250 |
| 5251 | gtggactcca | acgtcaaagg | gcgaaaaacc | gtctatcagg | gcgatggccc | 5300 |
| 5301 | actacgtgaa | ccatcaccct | aatcaagttt | tttggggtcg | aggtgccgta | 5350 |
| 5351 | aagcactaaa | tcggaaccct | aaagggagcc | cccgatttag | agcttgacgg | 5400 |
| 5401 | ggaaagccgg | cgaacgtggc | gagaaaggaa | gggaagaaag | cgaaaggagc | 5450 |
| 5451 | gggcgctagg | gcgctggcaa | gtgtagcggt | cacgctgcgc | gtaaccacca | 5500 |
| 5501 | cacccgccgc | gcttaatgcg | ccgctacagg | gcgcgtccca | ttcgcca | 5547 |
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pET-25b(+)
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