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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-24d(+)(pET24d)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pET-24d(+)(pET24d)
质粒名称: pET-24d(+)
其他名称:
质粒大小（bp）: 5307
抗性: Kanamycin
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-T7, C-His
5’测序引物:T7
5’测序引物序列:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Same as pET21abcd(+) but kanR; a,b,c,d vary by MCS; The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| T7_leader | 237 | 205 | |
| T7_transl_en_RBS | 261 | 245 | |
| lacO | 306 | 279 | |
| T7_promoter | 324 | 306 | |
| tet (300 – 563) | 356 | 619 | |
| pBRrevBam_primer | 427 | 408 | |
| lacI | 702 | 1793 | |
| tet (576 – 851) | 1852 | 2127 | |
| ROP | 2602 | 2793 | |
| pGEX_3_primer | 2809 | 2787 | |
| pBR322_origin | 3827 | 3208 | |
| KanR2 | 3933 | 4748 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 461 | 961 | |
| ORF frame 3 | 834 | 1793 | |
| ORF frame 3 | 3933 | 4748 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| NheI | 229 |
| NcoI | 234 |
| XbaI | 273 |
| BglII | 339 |
| BclI | 1075 |
| ApaI | 1272 |
| EcoRV | 1511 |
| HpaI | 1567 |
| FspI | 2143 |
| NruI | 4021 |
| ClaI | 4055 |
| XmaI | 4236 |
| SmaI | 4238 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccgacccat | ttgctgtcca | ccagtcatgc | tagccatggt | atatctcctt | 250 |
| 251 | cttaaagtta | aacaaaatta | tttctagagg | ggaattgtta | tccgctcaca | 300 |
| 301 | attcccctat | agtgagtcgt | attaatttcg | cgggatcgag | atctcgatcc | 350 |
| 351 | tctacgccgg | acgcatcgtg | gccggcatca | ccggcgccac | aggtgcggtt | 400 |
| 401 | gctggcgcct | atatcgccga | catcaccgat | ggggaagatc | gggctcgcca | 450 |
| 451 | cttcgggctc | atgagcgctt | gtttcggcgt | gggtatggtg | gcaggccccg | 500 |
| 501 | tggccggggg | actgttgggc | gccatctcct | tgcatgcacc | attccttgcg | 550 |
| 551 | gcggcggtgc | tcaacggcct | caacctacta | ctgggctgct | tcctaatgca | 600 |
| 601 | ggagtcgcat | aagggagagc | gtcgagatcc | cggacaccat | cgaatggcgc | 650 |
| 651 | aaaacctttc | gcggtatggc | atgatagcgc | ccggaagaga | gtcaattcag | 700 |
| 701 | ggtggtgaat | gtgaaaccag | taacgttata | cgatgtcgca | gagtatgccg | 750 |
| 751 | gtgtctctta | tcagaccgtt | tcccgcgtgg | tgaaccaggc | cagccacgtt | 800 |
| 801 | tctgcgaaaa | cgcgggaaaa | agtggaagcg | gcgatggcgg | agctgaatta | 850 |
| 851 | cattcccaac | cgcgtggcac | aacaactggc | gggcaaacag | tcgttgctga | 900 |
| 901 | ttggcgttgc | cacctccagt | ctggccctgc | acgcgccgtc | gcaaattgtc | 950 |
| 951 | gcggcgatta | aatctcgcgc | cgatcaactg | ggtgccagcg | tggtggtgtc | 1000 |
| 1001 | gatggtagaa | cgaagcggcg | tcgaagcctg | taaagcggcg | gtgcacaatc | 1050 |
| 1051 | ttctcgcgca | acgcgtcagt | gggctgatca | ttaactatcc | gctggatgac | 1100 |
| 1101 | caggatgcca | ttgctgtgga | agctgcctgc | actaatgttc | cggcgttatt | 1150 |
| 1151 | tcttgatgtc | tctgaccaga | cacccatcaa | cagtattatt | ttctcccatg | 1200 |
| 1201 | aagacggtac | gcgactgggc | gtggagcatc | tggtcgcatt | gggtcaccag | 1250 |
| 1251 | caaatcgcgc | tgttagcggg | cccattaagt | tctgtctcgg | cgcgtctgcg | 1300 |
| 1301 | tctggctggc | tggcataaat | atctcactcg | caatcaaatt | cagccgatag | 1350 |
| 1351 | cggaacggga | aggcgactgg | agtgccatgt | ccggttttca | acaaaccatg | 1400 |
| 1401 | caaatgctga | atgagggcat | cgttcccact | gcgatgctgg | ttgccaacga | 1450 |
| 1451 | tcagatggcg | ctgggcgcaa | tgcgcgccat | taccgagtcc | gggctgcgcg | 1500 |
| 1501 | ttggtgcgga | tatctcggta | gtgggatacg | acgataccga | agacagctca | 1550 |
| 1551 | tgttatatcc | cgccgttaac | caccatcaaa | caggattttc | gcctgctggg | 1600 |
| 1601 | gcaaaccagc | gtggaccgct | tgctgcaact | ctctcagggc | caggcggtga | 1650 |
| 1651 | agggcaatca | gctgttgccc | gtctcactgg | tgaaaagaaa | aaccaccctg | 1700 |
| 1701 | gcgcccaata | cgcaaaccgc | ctctccccgc | gcgttggccg | attcattaat | 1750 |
| 1751 | gcagctggca | cgacaggttt | cccgactgga | aagcgggcag | tgagcgcaac | 1800 |
| 1801 | gcaattaatg | taagttagct | cactcattag | gcaccgggat | ctcgaccgat | 1850 |
| 1851 | gcccttgaga | gccttcaacc | cagtcagctc | cttccggtgg | gcgcggggca | 1900 |
| 1901 | tgactatcgt | cgccgcactt | atgactgtct | tctttatcat | gcaactcgta | 1950 |
| 1951 | ggacaggtgc | cggcagcgct | ctgggtcatt | ttcggcgagg | accgctttcg | 2000 |
| 2001 | ctggagcgcg | acgatgatcg | gcctgtcgct | tgcggtattc | ggaatcttgc | 2050 |
| 2051 | acgccctcgc | tcaagccttc | gtcactggtc | ccgccaccaa | acgtttcggc | 2100 |
| 2101 | gagaagcagg | ccattatcgc | cggcatggcg | gccccacggg | tgcgcatgat | 2150 |
| 2151 | cgtgctcctg | tcgttgagga | cccggctagg | ctggcggggt | tgccttactg | 2200 |
| 2201 | gttagcagaa | tgaatcaccg | atacgcgagc | gaacgtgaag | cgactgctgc | 2250 |
| 2251 | tgcaaaacgt | ctgcgacctg | agcaacaaca | tgaatggtct | tcggtttccg | 2300 |
| 2301 | tgtttcgtaa | agtctggaaa | cgcggaagtc | agcgccctgc | accattatgt | 2350 |
| 2351 | tccggatctg | catcgcagga | tgctgctggc | taccctgtgg | aacacctaca | 2400 |
| 2401 | tctgtattaa | cgaagcgctg | gcattgaccc | tgagtgattt | ttctctggtc | 2450 |
| 2451 | ccgccgcatc | cataccgcca | gttgtttacc | ctcacaacgt | tccagtaacc | 2500 |
| 2501 | gggcatgttc | atcatcagta | acccgtatcg | tgagcatcct | ctctcgtttc | 2550 |
| 2551 | atcggtatca | ttacccccat | gaacagaaat | cccccttaca | cggaggcatc | 2600 |
| 2601 | agtgaccaaa | caggaaaaaa | ccgcccttaa | catggcccgc | tttatcagaa | 2650 |
| 2651 | gccagacatt | aacgcttctg | gagaaactca | acgagctgga | cgcggatgaa | 2700 |
| 2701 | caggcagaca | tctgtgaatc | gcttcacgac | cacgctgatg | agctttaccg | 2750 |
| 2751 | cagctgcctc | gcgcgtttcg | gtgatgacgg | tgaaaacctc | tgacacatgc | 2800 |
| 2801 | agctcccgga | gacggtcaca | gcttgtctgt | aagcggatgc | cgggagcaga | 2850 |
| 2851 | caagcccgtc | agggcgcgtc | agcgggtgtt | ggcgggtgtc | ggggcgcagc | 2900 |
| 2901 | catgacccag | tcacgtagcg | atagcggagt | gtatactggc | ttaactatgc | 2950 |
| 2951 | ggcatcagag | cagattgtac | tgagagtgca | ccatatatgc | ggtgtgaaat | 3000 |
| 3001 | accgcacaga | tgcgtaagga | gaaaataccg | catcaggcgc | tcttccgctt | 3050 |
| 3051 | cctcgctcac | tgactcgctg | cgctcggtcg | ttcggctgcg | gcgagcggta | 3100 |
| 3101 | tcagctcact | caaaggcggt | aatacggtta | tccacagaat | caggggataa | 3150 |
| 3151 | cgcaggaaag | aacatgtgag | caaaaggcca | gcaaaaggcc | aggaaccgta | 3200 |
| 3201 | aaaaggccgc | gttgctggcg | tttttccata | ggctccgccc | ccctgacgag | 3250 |
| 3251 | catcacaaaa | atcgacgctc | aagtcagagg | tggcgaaacc | cgacaggact | 3300 |
| 3301 | ataaagatac | caggcgtttc | cccctggaag | ctccctcgtg | cgctctcctg | 3350 |
| 3351 | ttccgaccct | gccgcttacc | ggatacctgt | ccgcctttct | cccttcggga | 3400 |
| 3401 | agcgtggcgc | tttctcatag | ctcacgctgt | aggtatctca | gttcggtgta | 3450 |
| 3451 | ggtcgttcgc | tccaagctgg | gctgtgtgca | cgaacccccc | gttcagcccg | 3500 |
| 3501 | accgctgcgc | cttatccggt | aactatcgtc | ttgagtccaa | cccggtaaga | 3550 |
| 3551 | cacgacttat | cgccactggc | agcagccact | ggtaacagga | ttagcagagc | 3600 |
| 3601 | gaggtatgta | ggcggtgcta | cagagttctt | gaagtggtgg | cctaactacg | 3650 |
| 3651 | gctacactag | aaggacagta | tttggtatct | gcgctctgct | gaagccagtt | 3700 |
| 3701 | accttcggaa | aaagagttgg | tagctcttga | tccggcaaac | aaaccaccgc | 3750 |
| 3751 | tggtagcggt | ggtttttttg | tttgcaagca | gcagattacg | cgcagaaaaa | 3800 |
| 3801 | aaggatctca | agaagatcct | ttgatctttt | ctacggggtc | tgacgctcag | 3850 |
| 3851 | tggaacgaaa | actcacgtta | agggattttg | gtcatgaaca | ataaaactgt | 3900 |
| 3901 | ctgcttacat | aaacagtaat | acaaggggtg | ttatgagcca | tattcaacgg | 3950 |
| 3951 | gaaacgtctt | gctctaggcc | gcgattaaat | tccaacatgg | atgctgattt | 4000 |
| 4001 | atatgggtat | aaatgggctc | gcgataatgt | cgggcaatca | ggtgcgacaa | 4050 |
| 4051 | tctatcgatt | gtatgggaag | cccgatgcgc | cagagttgtt | tctgaaacat | 4100 |
| 4101 | ggcaaaggta | gcgttgccaa | tgatgttaca | gatgagatgg | tcagactaaa | 4150 |
| 4151 | ctggctgacg | gaatttatgc | ctcttccgac | catcaagcat | tttatccgta | 4200 |
| 4201 | ctcctgatga | tgcatggtta | ctcaccactg | cgatccccgg | gaaaacagca | 4250 |
| 4251 | ttccaggtat | tagaagaata | tcctgattca | ggtgaaaata | ttgttgatgc | 4300 |
| 4301 | gctggcagtg | ttcctgcgcc | ggttgcattc | gattcctgtt | tgtaattgtc | 4350 |
| 4351 | cttttaacag | cgatcgcgta | tttcgtctcg | ctcaggcgca | atcacgaatg | 4400 |
| 4401 | aataacggtt | tggttgatgc | gagtgatttt | gatgacgagc | gtaatggctg | 4450 |
| 4451 | gcctgttgaa | caagtctgga | aagaaatgca | taaacttttg | ccattctcac | 4500 |
| 4501 | cggattcagt | cgtcactcat | ggtgatttct | cacttgataa | ccttattttt | 4550 |
| 4551 | gacgagggga | aattaatagg | ttgtattgat | gttggacgag | tcggaatcgc | 4600 |
| 4601 | agaccgatac | caggatcttg | ccatcctatg | gaactgcctc | ggtgagtttt | 4650 |
| 4651 | ctccttcatt | acagaaacgg | ctttttcaaa | aatatggtat | tgataatcct | 4700 |
| 4701 | gatatgaata | aattgcagtt | tcatttgatg | ctcgatgagt | ttttctaaga | 4750 |
| 4751 | attaattcat | gagcggatac | atatttgaat | gtatttagaa | aaataaacaa | 4800 |
| 4801 | ataggggttc | cgcgcacatt | tccccgaaaa | gtgccacctg | aaattgtaaa | 4850 |
| 4851 | cgttaatatt | ttgttaaaat | tcgcgttaaa | tttttgttaa | atcagctcat | 4900 |
| 4901 | tttttaacca | ataggccgaa | atcggcaaaa | tcccttataa | atcaaaagaa | 4950 |
| 4951 | tagaccgaga | tagggttgag | tgttgttcca | gtttggaaca | agagtccact | 5000 |
| 5001 | attaaagaac | gtggactcca | acgtcaaagg | gcgaaaaacc | gtctatcagg | 5050 |
| 5051 | gcgatggccc | actacgtgaa | ccatcaccct | aatcaagttt | tttggggtcg | 5100 |
| 5101 | aggtgccgta | aagcactaaa | tcggaaccct | aaagggagcc | cccgatttag | 5150 |
| 5151 | agcttgacgg | ggaaagccgg | cgaacgtggc | gagaaaggaa | gggaagaaag | 5200 |
| 5201 | cgaaaggagc | gggcgctagg | gcgctggcaa | gtgtagcggt | cacgctgcgc | 5250 |
| 5251 | gtaaccacca | cacccgccgc | gcttaatgcg | ccgctacagg | gcgcgtccca | 5300 |
| 5301 | ttcgcca | 5307 |
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pET-24d(+)(pET24d)
¥1 - 1880
