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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-24a(+)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pET-24a(+)
质粒名称: pET-24a(+)
其他名称: pET24a, pet 24a
质粒大小（bp）: 5310
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-T7, C-His
5′ 测序引物:T7
5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Same as pET21abcd(+) but kanR; a,b,c,d vary by MCS; The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| T7_leader | 239 | 207 | |
| Xpress_fwd_primer | 240 | 222 | |
| T7_transl_en_RBS | 264 | 248 | |
| lacO | 309 | 282 | |
| T7_promoter | 327 | 309 | |
| tet (300 – 563) | 359 | 622 | |
| pBRrevBam_primer | 430 | 411 | |
| lacI | 705 | 1796 | |
| tet (576 – 851) | 1855 | 2130 | |
| ROP | 2605 | 2796 | |
| pGEX_3_primer | 2812 | 2790 | |
| pBR322_origin | 3830 | 3211 | |
| KanR2 | 3936 | 4751 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 464 | 964 | |
| ORF frame 3 | 837 | 1796 | |
| ORF frame 3 | 3936 | 4751 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| NheI | 231 |
| NdeI | 238 |
| XbaI | 276 |
| BglII | 342 |
| BclI | 1078 |
| ApaI | 1275 |
| EcoRV | 1514 |
| HpaI | 1570 |
| FspI | 2146 |
| NruI | 4024 |
| ClaI | 4058 |
| XmaI | 4239 |
| SmaI | 4241 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccgcgaccc | atttgctgtc | caccagtcat | gctagccata | tgtatatctc | 250 |
| 251 | cttcttaaag | ttaaacaaaa | ttatttctag | aggggaattg | ttatccgctc | 300 |
| 301 | acaattcccc | tatagtgagt | cgtattaatt | tcgcgggatc | gagatctcga | 350 |
| 351 | tcctctacgc | cggacgcatc | gtggccggca | tcaccggcgc | cacaggtgcg | 400 |
| 401 | gttgctggcg | cctatatcgc | cgacatcacc | gatggggaag | atcgggctcg | 450 |
| 451 | ccacttcggg | ctcatgagcg | cttgtttcgg | cgtgggtatg | gtggcaggcc | 500 |
| 501 | ccgtggccgg | gggactgttg | ggcgccatct | ccttgcatgc | accattcctt | 550 |
| 551 | gcggcggcgg | tgctcaacgg | cctcaaccta | ctactgggct | gcttcctaat | 600 |
| 601 | gcaggagtcg | cataagggag | agcgtcgaga | tcccggacac | catcgaatgg | 650 |
| 651 | cgcaaaacct | ttcgcggtat | ggcatgatag | cgcccggaag | agagtcaatt | 700 |
| 701 | cagggtggtg | aatgtgaaac | cagtaacgtt | atacgatgtc | gcagagtatg | 750 |
| 751 | ccggtgtctc | ttatcagacc | gtttcccgcg | tggtgaacca | ggccagccac | 800 |
| 801 | gtttctgcga | aaacgcggga | aaaagtggaa | gcggcgatgg | cggagctgaa | 850 |
| 851 | ttacattccc | aaccgcgtgg | cacaacaact | ggcgggcaaa | cagtcgttgc | 900 |
| 901 | tgattggcgt | tgccacctcc | agtctggccc | tgcacgcgcc | gtcgcaaatt | 950 |
| 951 | gtcgcggcga | ttaaatctcg | cgccgatcaa | ctgggtgcca | gcgtggtggt | 1000 |
| 1001 | gtcgatggta | gaacgaagcg | gcgtcgaagc | ctgtaaagcg | gcggtgcaca | 1050 |
| 1051 | atcttctcgc | gcaacgcgtc | agtgggctga | tcattaacta | tccgctggat | 1100 |
| 1101 | gaccaggatg | ccattgctgt | ggaagctgcc | tgcactaatg | ttccggcgtt | 1150 |
| 1151 | atttcttgat | gtctctgacc | agacacccat | caacagtatt | attttctccc | 1200 |
| 1201 | atgaagacgg | tacgcgactg | ggcgtggagc | atctggtcgc | attgggtcac | 1250 |
| 1251 | cagcaaatcg | cgctgttagc | gggcccatta | agttctgtct | cggcgcgtct | 1300 |
| 1301 | gcgtctggct | ggctggcata | aatatctcac | tcgcaatcaa | attcagccga | 1350 |
| 1351 | tagcggaacg | ggaaggcgac | tggagtgcca | tgtccggttt | tcaacaaacc | 1400 |
| 1401 | atgcaaatgc | tgaatgaggg | catcgttccc | actgcgatgc | tggttgccaa | 1450 |
| 1451 | cgatcagatg | gcgctgggcg | caatgcgcgc | cattaccgag | tccgggctgc | 1500 |
| 1501 | gcgttggtgc | ggatatctcg | gtagtgggat | acgacgatac | cgaagacagc | 1550 |
| 1551 | tcatgttata | tcccgccgtt | aaccaccatc | aaacaggatt | ttcgcctgct | 1600 |
| 1601 | ggggcaaacc | agcgtggacc | gcttgctgca | actctctcag | ggccaggcgg | 1650 |
| 1651 | tgaagggcaa | tcagctgttg | cccgtctcac | tggtgaaaag | aaaaaccacc | 1700 |
| 1701 | ctggcgccca | atacgcaaac | cgcctctccc | cgcgcgttgg | ccgattcatt | 1750 |
| 1751 | aatgcagctg | gcacgacagg | tttcccgact | ggaaagcggg | cagtgagcgc | 1800 |
| 1801 | aacgcaatta | atgtaagtta | gctcactcat | taggcaccgg | gatctcgacc | 1850 |
| 1851 | gatgcccttg | agagccttca | acccagtcag | ctccttccgg | tgggcgcggg | 1900 |
| 1901 | gcatgactat | cgtcgccgca | cttatgactg | tcttctttat | catgcaactc | 1950 |
| 1951 | gtaggacagg | tgccggcagc | gctctgggtc | attttcggcg | aggaccgctt | 2000 |
| 2001 | tcgctggagc | gcgacgatga | tcggcctgtc | gcttgcggta | ttcggaatct | 2050 |
| 2051 | tgcacgccct | cgctcaagcc | ttcgtcactg | gtcccgccac | caaacgtttc | 2100 |
| 2101 | ggcgagaagc | aggccattat | cgccggcatg | gcggccccac | gggtgcgcat | 2150 |
| 2151 | gatcgtgctc | ctgtcgttga | ggacccggct | aggctggcgg | ggttgcctta | 2200 |
| 2201 | ctggttagca | gaatgaatca | ccgatacgcg | agcgaacgtg | aagcgactgc | 2250 |
| 2251 | tgctgcaaaa | cgtctgcgac | ctgagcaaca | acatgaatgg | tcttcggttt | 2300 |
| 2301 | ccgtgtttcg | taaagtctgg | aaacgcggaa | gtcagcgccc | tgcaccatta | 2350 |
| 2351 | tgttccggat | ctgcatcgca | ggatgctgct | ggctaccctg | tggaacacct | 2400 |
| 2401 | acatctgtat | taacgaagcg | ctggcattga | ccctgagtga | tttttctctg | 2450 |
| 2451 | gtcccgccgc | atccataccg | ccagttgttt | accctcacaa | cgttccagta | 2500 |
| 2501 | accgggcatg | ttcatcatca | gtaacccgta | tcgtgagcat | cctctctcgt | 2550 |
| 2551 | ttcatcggta | tcattacccc | catgaacaga | aatccccctt | acacggaggc | 2600 |
| 2601 | atcagtgacc | aaacaggaaa | aaaccgccct | taacatggcc | cgctttatca | 2650 |
| 2651 | gaagccagac | attaacgctt | ctggagaaac | tcaacgagct | ggacgcggat | 2700 |
| 2701 | gaacaggcag | acatctgtga | atcgcttcac | gaccacgctg | atgagcttta | 2750 |
| 2751 | ccgcagctgc | ctcgcgcgtt | tcggtgatga | cggtgaaaac | ctctgacaca | 2800 |
| 2801 | tgcagctccc | ggagacggtc | acagcttgtc | tgtaagcgga | tgccgggagc | 2850 |
| 2851 | agacaagccc | gtcagggcgc | gtcagcgggt | gttggcgggt | gtcggggcgc | 2900 |
| 2901 | agccatgacc | cagtcacgta | gcgatagcgg | agtgtatact | ggcttaacta | 2950 |
| 2951 | tgcggcatca | gagcagattg | tactgagagt | gcaccatata | tgcggtgtga | 3000 |
| 3001 | aataccgcac | agatgcgtaa | ggagaaaata | ccgcatcagg | cgctcttccg | 3050 |
| 3051 | cttcctcgct | cactgactcg | ctgcgctcgg | tcgttcggct | gcggcgagcg | 3100 |
| 3101 | gtatcagctc | actcaaaggc | ggtaatacgg | ttatccacag | aatcagggga | 3150 |
| 3151 | taacgcagga | aagaacatgt | gagcaaaagg | ccagcaaaag | gccaggaacc | 3200 |
| 3201 | gtaaaaaggc | cgcgttgctg | gcgtttttcc | ataggctccg | cccccctgac | 3250 |
| 3251 | gagcatcaca | aaaatcgacg | ctcaagtcag | aggtggcgaa | acccgacagg | 3300 |
| 3301 | actataaaga | taccaggcgt | ttccccctgg | aagctccctc | gtgcgctctc | 3350 |
| 3351 | ctgttccgac | cctgccgctt | accggatacc | tgtccgcctt | tctcccttcg | 3400 |
| 3401 | ggaagcgtgg | cgctttctca | tagctcacgc | tgtaggtatc | tcagttcggt | 3450 |
| 3451 | gtaggtcgtt | cgctccaagc | tgggctgtgt | gcacgaaccc | cccgttcagc | 3500 |
| 3501 | ccgaccgctg | cgccttatcc | ggtaactatc | gtcttgagtc | caacccggta | 3550 |
| 3551 | agacacgact | tatcgccact | ggcagcagcc | actggtaaca | ggattagcag | 3600 |
| 3601 | agcgaggtat | gtaggcggtg | ctacagagtt | cttgaagtgg | tggcctaact | 3650 |
| 3651 | acggctacac | tagaaggaca | gtatttggta | tctgcgctct | gctgaagcca | 3700 |
| 3701 | gttaccttcg | gaaaaagagt | tggtagctct | tgatccggca | aacaaaccac | 3750 |
| 3751 | cgctggtagc | ggtggttttt | ttgtttgcaa | gcagcagatt | acgcgcagaa | 3800 |
| 3801 | aaaaaggatc | tcaagaagat | cctttgatct | tttctacggg | gtctgacgct | 3850 |
| 3851 | cagtggaacg | aaaactcacg | ttaagggatt | ttggtcatga | acaataaaac | 3900 |
| 3901 | tgtctgctta | cataaacagt | aatacaaggg | gtgttatgag | ccatattcaa | 3950 |
| 3951 | cgggaaacgt | cttgctctag | gccgcgatta | aattccaaca | tggatgctga | 4000 |
| 4001 | tttatatggg | tataaatggg | ctcgcgataa | tgtcgggcaa | tcaggtgcga | 4050 |
| 4051 | caatctatcg | attgtatggg | aagcccgatg | cgccagagtt | gtttctgaaa | 4100 |
| 4101 | catggcaaag | gtagcgttgc | caatgatgtt | acagatgaga | tggtcagact | 4150 |
| 4151 | aaactggctg | acggaattta | tgcctcttcc | gaccatcaag | cattttatcc | 4200 |
| 4201 | gtactcctga | tgatgcatgg | ttactcacca | ctgcgatccc | cgggaaaaca | 4250 |
| 4251 | gcattccagg | tattagaaga | atatcctgat | tcaggtgaaa | atattgttga | 4300 |
| 4301 | tgcgctggca | gtgttcctgc | gccggttgca | ttcgattcct | gtttgtaatt | 4350 |
| 4351 | gtccttttaa | cagcgatcgc | gtatttcgtc | tcgctcaggc | gcaatcacga | 4400 |
| 4401 | atgaataacg | gtttggttga | tgcgagtgat | tttgatgacg | agcgtaatgg | 4450 |
| 4451 | ctggcctgtt | gaacaagtct | ggaaagaaat | gcataaactt | ttgccattct | 4500 |
| 4501 | caccggattc | agtcgtcact | catggtgatt | tctcacttga | taaccttatt | 4550 |
| 4551 | tttgacgagg | ggaaattaat | aggttgtatt | gatgttggac | gagtcggaat | 4600 |
| 4601 | cgcagaccga | taccaggatc | ttgccatcct | atggaactgc | ctcggtgagt | 4650 |
| 4651 | tttctccttc | attacagaaa | cggctttttc | aaaaatatgg | tattgataat | 4700 |
| 4701 | cctgatatga | ataaattgca | gtttcatttg | atgctcgatg | agtttttcta | 4750 |
| 4751 | agaattaatt | catgagcgga | tacatatttg | aatgtattta | gaaaaataaa | 4800 |
| 4801 | caaatagggg | ttccgcgcac | atttccccga | aaagtgccac | ctgaaattgt | 4850 |
| 4851 | aaacgttaat | attttgttaa | aattcgcgtt | aaatttttgt | taaatcagct | 4900 |
| 4901 | cattttttaa | ccaataggcc | gaaatcggca | aaatccctta | taaatcaaaa | 4950 |
| 4951 | gaatagaccg | agatagggtt | gagtgttgtt | ccagtttgga | acaagagtcc | 5000 |
| 5001 | actattaaag | aacgtggact | ccaacgtcaa | agggcgaaaa | accgtctatc | 5050 |
| 5051 | agggcgatgg | cccactacgt | gaaccatcac | cctaatcaag | ttttttgggg | 5100 |
| 5101 | tcgaggtgcc | gtaaagcact | aaatcggaac | cctaaaggga | gcccccgatt | 5150 |
| 5151 | tagagcttga | cggggaaagc | cggcgaacgt | ggcgagaaag | gaagggaaga | 5200 |
| 5201 | aagcgaaagg | agcgggcgct | agggcgctgg | caagtgtagc | ggtcacgctg | 5250 |
| 5251 | cgcgtaacca | ccacacccgc | cgcgcttaat | gcgccgctac | agggcgcgtc | 5300 |
| 5301 | ccattcgcca | 5310 |
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pET-24a(+)
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