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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-16b
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pET-16b
质粒名称: pET-16b
其他名称:
质粒大小（bp）: 5711
抗性: Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: 非病毒
启动子: T7
稳转或瞬转: 瞬时表达
组成型或诱导性: 组成型
表达水平: 高
蛋白标签: N-His, N-Factor Xa蛋白酶切位点
5’测序引物:T7
5’测序引物序列:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性:
质粒图谱:
质粒序列:
| 1 | ttctcatgtt | tgacagctta | tcatcgataa | gctttaatgc | ggtagtttat | 50 |
| 51 | cacagttaaa | ttgctaacgc | agtcaggcac | cgtgtatgaa | atctaacaat | 100 |
| 101 | gcgctcatcg | tcatcctcgg | caccgtcacc | ctggatgctg | taggcatagg | 150 |
| 151 | cttggttatg | ccggtactgc | cgggcctctt | gcgggatatc | cggatatagt | 200 |
| 201 | tcctcctttc | agcaaaaaac | ccctcaagac | ccgtttagag | gccccaaggg | 250 |
| 251 | gttatgctag | ttattgctca | gcggtggcag | cagccaactc | agcttccttt | 300 |
| 301 | cgggctttgt | tagcagccgg | atcctcgagc | atatgacgac | cttcgatatg | 350 |
| 351 | gccgctgctg | tgatgatgat | gatgatgatg | atgatgatgg | cccatggtat | 400 |
| 401 | atctccttct | taaagttaaa | caaaattatt | tctagagggg | aattgttatc | 450 |
| 451 | cgctcacaat | tcccctatag | tgagtcgtat | taatttcgcg | ggatcgagat | 500 |
| 501 | ctcgatcctc | tacgccggac | gcatcgtggc | cggcatcacc | ggcgccacag | 550 |
| 551 | gtgcggttgc | tggcgcctat | atcgccgaca | tcaccgatgg | ggaagatcgg | 600 |
| 601 | gctcgccact | tcgggctcat | gagcgcttgt | ttcggcgtgg | gtatggtggc | 650 |
| 651 | aggccccgtg | gccgggggac | tgttgggcgc | catctccttg | catgcaccat | 700 |
| 701 | tccttgcggc | ggcggtgctc | aacggcctca | acctactact | gggctgcttc | 750 |
| 751 | ctaatgcagg | agtcgcataa | gggagagcgt | cgagatcccg | gacaccatcg | 800 |
| 801 | aatggcgcaa | aacctttcgc | ggtatggcat | gatagcgccc | ggaagagagt | 850 |
| 851 | caattcaggg | tggtgaatgt | gaaaccagta | acgttatacg | atgtcgcaga | 900 |
| 901 | gtatgccggt | gtctcttatc | agaccgtttc | ccgcgtggtg | aaccaggcca | 950 |
| 951 | gccacgtttc | tgcgaaaacg | cgggaaaaag | tggaagcggc | gatggcggag | 1000 |
| 1001 | ctgaattaca | ttcccaaccg | cgtggcacaa | caactggcgg | gcaaacagtc | 1050 |
| 1051 | gttgctgatt | ggcgttgcca | cctccagtct | ggccctgcac | gcgccgtcgc | 1100 |
| 1101 | aaattgtcgc | ggcgattaaa | tctcgcgccg | atcaactggg | tgccagcgtg | 1150 |
| 1151 | gtggtgtcga | tggtagaacg | aagcggcgtc | gaagcctgta | aagcggcggt | 1200 |
| 1201 | gcacaatctt | ctcgcgcaac | gcgtcagtgg | gctgatcatt | aactatccgc | 1250 |
| 1251 | tggatgacca | ggatgccatt | gctgtggaag | ctgcctgcac | taatgttccg | 1300 |
| 1301 | gcgttatttc | ttgatgtctc | tgaccagaca | cccatcaaca | gtattatttt | 1350 |
| 1351 | ctcccatgaa | gacggtacgc | gactgggcgt | ggagcatctg | gtcgcattgg | 1400 |
| 1401 | gtcaccagca | aatcgcgctg | ttagcgggcc | cattaagttc | tgtctcggcg | 1450 |
| 1451 | cgtctgcgtc | tggctggctg | gcataaatat | ctcactcgca | atcaaattca | 1500 |
| 1501 | gccgatagcg | gaacgggaag | gcgactggag | tgccatgtcc | ggttttcaac | 1550 |
| 1551 | aaaccatgca | aatgctgaat | gagggcatcg | ttcccactgc | gatgctggtt | 1600 |
| 1601 | gccaacgatc | agatggcgct | gggcgcaatg | cgcgccatta | ccgagtccgg | 1650 |
| 1651 | gctgcgcgtt | ggtgcggata | tctcggtagt | gggatacgac | gataccgaag | 1700 |
| 1701 | acagctcatg | ttatatcccg | ccgttaacca | ccatcaaaca | ggattttcgc | 1750 |
| 1751 | ctgctggggc | aaaccagcgt | ggaccgcttg | ctgcaactct | ctcagggcca | 1800 |
| 1801 | ggcggtgaag | ggcaatcagc | tgttgcccgt | ctcactggtg | aaaagaaaaa | 1850 |
| 1851 | ccaccctggc | gcccaatacg | caaaccgcct | ctccccgcgc | gttggccgat | 1900 |
| 1901 | tcattaatgc | agctggcacg | acaggtttcc | cgactggaaa | gcgggcagtg | 1950 |
| 1951 | agcgcaacgc | aattaatgta | agttagctca | ctcattaggc | accgggatct | 2000 |
| 2001 | cgaccgatgc | ccttgagagc | cttcaaccca | gtcagctcct | tccggtgggc | 2050 |
| 2051 | gcggggcatg | actatcgtcg | ccgcacttat | gactgtcttc | tttatcatgc | 2100 |
| 2101 | aactcgtagg | acaggtgccg | gcagcgctct | gggtcatttt | cggcgaggac | 2150 |
| 2151 | cgctttcgct | ggagcgcgac | gatgatcggc | ctgtcgcttg | cggtattcgg | 2200 |
| 2201 | aatcttgcac | gccctcgctc | aagccttcgt | cactggtccc | gccaccaaac | 2250 |
| 2251 | gtttcggcga | gaagcaggcc | attatcgccg | gcatggcggc | cgacgcgctg | 2300 |
| 2301 | ggctacgtct | tgctggcgtt | cgcgacgcga | ggctggatgg | ccttccccat | 2350 |
| 2351 | tatgattctt | ctcgcttccg | gcggcatcgg | gatgcccgcg | ttgcaggcca | 2400 |
| 2401 | tgctgtccag | gcaggtagat | gacgaccatc | agggacagct | tcaaggatcg | 2450 |
| 2451 | ctcgcggctc | ttaccagcct | aacttcgatc | actggaccgc | tgatcgtcac | 2500 |
| 2501 | ggcgatttat | gccgcctcgg | cgagcacatg | gaacgggttg | gcatggattg | 2550 |
| 2551 | taggcgccgc | cctatacctt | gtctgcctcc | ccgcgttgcg | tcgcggtgca | 2600 |
| 2601 | tggagccggg | ccacctcgac | ctgaatggaa | gccggcggca | cctcgctaac | 2650 |
| 2651 | ggattcacca | ctccaagaat | tggagccaat | caattcttgc | ggagaactgt | 2700 |
| 2701 | gaatgcgcaa | accaaccctt | ggcagaacat | atccatcgcg | tccgccatct | 2750 |
| 2751 | ccagcagccg | cacgcggcgc | atctcgggca | gcgttgggtc | ctggccacgg | 2800 |
| 2801 | gtgcgcatga | tcgtgctcct | gtcgttgagg | acccggctag | gctggcgggg | 2850 |
| 2851 | ttgccttact | ggttagcaga | atgaatcacc | gatacgcgag | cgaacgtgaa | 2900 |
| 2901 | gcgactgctg | ctgcaaaacg | tctgcgacct | gagcaacaac | atgaatggtc | 2950 |
| 2951 | ttcggtttcc | gtgtttcgta | aagtctggaa | acgcggaagt | cagcgccctg | 3000 |
| 3001 | caccattatg | ttccggatct | gcatcgcagg | atgctgctgg | ctaccctgtg | 3050 |
| 3051 | gaacacctac | atctgtatta | acgaagcgct | ggcattgacc | ctgagtgatt | 3100 |
| 3101 | tttctctggt | cccgccgcat | ccataccgcc | agttgtttac | cctcacaacg | 3150 |
| 3151 | ttccagtaac | cgggcatgtt | catcatcagt | aacccgtatc | gtgagcatcc | 3200 |
| 3201 | tctctcgttt | catcggtatc | attaccccca | tgaacagaaa | tcccccttac | 3250 |
| 3251 | acggaggcat | cagtgaccaa | acaggaaaaa | accgccctta | acatggcccg | 3300 |
| 3301 | ctttatcaga | agccagacat | taacgcttct | ggagaaactc | aacgagctgg | 3350 |
| 3351 | acgcggatga | acaggcagac | atctgtgaat | cgcttcacga | ccacgctgat | 3400 |
| 3401 | gagctttacc | gcagctgcct | cgcgcgtttc | ggtgatgacg | gtgaaaacct | 3450 |
| 3451 | ctgacacatg | cagctcccgg | agacggtcac | agcttgtctg | taagcggatg | 3500 |
| 3501 | ccgggagcag | acaagcccgt | cagggcgcgt | cagcgggtgt | tggcgggtgt | 3550 |
| 3551 | cggggcgcag | ccatgaccca | gtcacgtagc | gatagcggag | tgtatactgg | 3600 |
| 3601 | cttaactatg | cggcatcaga | gcagattgta | ctgagagtgc | accatatatg | 3650 |
| 3651 | cggtgtgaaa | taccgcacag | atgcgtaagg | agaaaatacc | gcatcaggcg | 3700 |
| 3701 | ctcttccgct | tcctcgctca | ctgactcgct | gcgctcggtc | gttcggctgc | 3750 |
| 3751 | ggcgagcggt | atcagctcac | tcaaaggcgg | taatacggtt | atccacagaa | 3800 |
| 3801 | tcaggggata | acgcaggaaa | gaacatgtga | gcaaaaggcc | agcaaaaggc | 3850 |
| 3851 | caggaaccgt | aaaaaggccg | cgttgctggc | gtttttccat | aggctccgcc | 3900 |
| 3901 | cccctgacga | gcatcacaaa | aatcgacgct | caagtcagag | gtggcgaaac | 3950 |
| 3951 | ccgacaggac | tataaagata | ccaggcgttt | ccccctggaa | gctccctcgt | 4000 |
| 4001 | gcgctctcct | gttccgaccc | tgccgcttac | cggatacctg | tccgcctttc | 4050 |
| 4051 | tcccttcggg | aagcgtggcg | ctttctcata | gctcacgctg | taggtatctc | 4100 |
| 4101 | agttcggtgt | aggtcgttcg | ctccaagctg | ggctgtgtgc | acgaaccccc | 4150 |
| 4151 | cgttcagccc | gaccgctgcg | ccttatccgg | taactatcgt | cttgagtcca | 4200 |
| 4201 | acccggtaag | acacgactta | tcgccactgg | cagcagccac | tggtaacagg | 4250 |
| 4251 | attagcagag | cgaggtatgt | aggcggtgct | acagagttct | tgaagtggtg | 4300 |
| 4301 | gcctaactac | ggctacacta | gaaggacagt | atttggtatc | tgcgctctgc | 4350 |
| 4351 | tgaagccagt | taccttcgga | aaaagagttg | gtagctcttg | atccggcaaa | 4400 |
| 4401 | caaaccaccg | ctggtagcgg | tggttttttt | gtttgcaagc | agcagattac | 4450 |
| 4451 | gcgcagaaaa | aaaggatctc | aagaagatcc | tttgatcttt | tctacggggt | 4500 |
| 4501 | ctgacgctca | gtggaacgaa | aactcacgtt | aagggatttt | ggtcatgaga | 4550 |
| 4551 | ttatcaaaaa | ggatcttcac | ctagatcctt | ttaaattaaa | aatgaagttt | 4600 |
| 4601 | taaatcaatc | taaagtatat | atgagtaaac | ttggtctgac | agttaccaat | 4650 |
| 4651 | gcttaatcag | tgaggcacct | atctcagcga | tctgtctatt | tcgttcatcc | 4700 |
| 4701 | atagttgcct | gactccccgt | cgtgtagata | actacgatac | gggagggctt | 4750 |
| 4751 | accatctggc | cccagtgctg | caatgatacc | gcgagaccca | cgctcaccgg | 4800 |
| 4801 | ctccagattt | atcagcaata | aaccagccag | ccggaagggc | cgagcgcaga | 4850 |
| 4851 | agtggtcctg | caactttatc | cgcctccatc | cagtctatta | attgttgccg | 4900 |
| 4901 | ggaagctaga | gtaagtagtt | cgccagttaa | tagtttgcgc | aacgttgttg | 4950 |
| 4951 | ccattgctgc | aggcatcgtg | gtgtcacgct | cgtcgtttgg | tatggcttca | 5000 |
| 5001 | ttcagctccg | gttcccaacg | atcaaggcga | gttacatgat | cccccatgtt | 5050 |
| 5051 | gtgcaaaaaa | gcggttagct | ccttcggtcc | tccgatcgtt | gtcagaagta | 5100 |
| 5101 | agttggccgc | agtgttatca | ctcatggtta | tggcagcact | gcataattct | 5150 |
| 5151 | cttactgtca | tgccatccgt | aagatgcttt | tctgtgactg | gtgagtactc | 5200 |
| 5201 | aaccaagtca | ttctgagaat | agtgtatgcg | gcgaccgagt | tgctcttgcc | 5250 |
| 5251 | cggcgtcaac | acgggataat | accgcgccac | atagcagaac | tttaaaagtg | 5300 |
| 5301 | ctcatcattg | gaaaacgttc | ttcggggcga | aaactctcaa | ggatcttacc | 5350 |
| 5351 | gctgttgaga | tccagttcga | tgtaacccac | tcgtgcaccc | aactgatctt | 5400 |
| 5401 | cagcatcttt | tactttcacc | agcgtttctg | ggtgagcaaa | aacaggaagg | 5450 |
| 5451 | caaaatgccg | caaaaaaggg | aataagggcg | acacggaaat | gttgaatact | 5500 |
| 5501 | catactcttc | ctttttcaat | attattgaag | catttatcag | ggttattgtc | 5550 |
| 5551 | tcatgagcgg | atacatattt | gaatgtattt | agaaaaataa | acaaataggg | 5600 |
| 5601 | gttccgcgca | catttccccg | aaaagtgcca | cctgacgtct | aagaaaccat | 5650 |
| 5651 | tattatcatg | acattaacct | ataaaaatag | gcgtatcacg | aggccctttc | 5700 |
| 5701 | gtcttcaaga | a | 5711 |
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pET-16b
¥1 - 1880
