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- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pCEP4
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pCEP4
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10186)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, August 22, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..10186
/organism=”synthetic DNA construct”
/mol_type=”other DNA”
enhancer 4..383
/note=”CMV enhancer”
/note=”human cytomegalovirus immediate early enhancer”
promoter 384..587
/note=”CMV promoter”
/note=”human cytomegalovirus (CMV) immediate early
promoter”
polyA_signal 672..806
/note=”SV40 poly(A) signal”
/note=”SV40 polyadenylation signal”
rep_origin 1529..3318
/note=”oriP”
/note=”Epstein-Barr virus oriP replication origin (Yates et
al., 2000)”
promoter 6066..6170
/gene=”bla”
/note=”AmpR promoter”
CDS 6171..7031
/codon_start=1
/gene=”bla”
/product=”beta-lactamase”
/note=”AmpR”
/note=”confers resistance to ampicillin, carbenicillin, and
related antibiotics”
/translation=”MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW”
rep_origin 7202..7790
/direction=RIGHT
/note=”ori”
/note=”high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication”
promoter 8221..8366
/note=”HSV TK promoter”
/note=”herpes simplex virus thymidine kinase promoter”
CDS 8409..9446
/codon_start=1
/product=”hygromycin B phosphotransferase”
/note=”HygR”
/note=”confers resistance to hygromycin”
/translation=”MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN”
polyA_signal 9488..9536
/note=”HSV TK poly(A) signal”
/note=”herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)”
ORIGIN
1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctcgaggc
661 cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa accacaacta
721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
781 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
841 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
901 ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
1021 tcaggcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga
1081 tcgatgcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
1141 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
1201 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
1261 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
1321 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
1381 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
1441 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
1501 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
1561 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
1621 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
1681 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
1741 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
1801 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
1861 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
1921 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
1981 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
2041 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
2101 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
2161 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
2221 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
2281 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
2341 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
2401 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
2461 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
2521 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
2581 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
2641 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
2701 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
2761 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
2821 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
2881 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
2941 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
3001 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
3061 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
3121 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
3181 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag
3241 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg
3301 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct
3361 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag
3421 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt
3481 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat
3541 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg
3601 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt
3661 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc
3721 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag
3781 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat
3841 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg
3901 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg
3961 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata
4021 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca
4081 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac
4141 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc
4201 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca
4261 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta
4321 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg
4381 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg
4441 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac
4501 ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc
4561 ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
4621 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
4681 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
4741 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
4801 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc
4861 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc
4921 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
4981 ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
5041 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
5101 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc
5161 ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
5221 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg
5281 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac
5341 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc
5401 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac
5461 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc
5521 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat
5581 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca
5641 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc
5701 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc
5761 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg
5821 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga
5881 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat
5941 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
6001 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
6061 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
6121 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
6181 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
6241 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
6301 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
6361 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
6421 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
6481 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
6541 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
6601 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
6661 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
6721 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
6781 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
6841 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
6901 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
6961 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
7021 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
7081 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
7141 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
7201 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
7261 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
7321 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
7381 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
7441 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
7501 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
7561 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
7621 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
7681 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
7741 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
7801 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt
7861 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
7921 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
7981 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
8041 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
8101 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt
8161 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg
8221 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
8281 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
8341 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
8401 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
8461 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
8521 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
8581 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
8641 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
8701 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
8761 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
8821 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
8881 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
8941 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
9001 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
9061 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
9121 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
9181 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
9241 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
9301 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
9361 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
9421 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
9481 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
9541 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
9601 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
9661 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta
9721 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
9781 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
9841 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
9901 cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc
9961 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
10021 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
10081 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
10141 cataatatgt acatttatat tggctcatgt ccaatatgac cgccat
质粒名称: pCEP4
其他名称:
质粒大小（bp）: 10186
抗性: Ampicillin
质粒类型: 哺乳动物细胞表达载体
是否病毒: 非病毒
启动子: CMV
稳转或瞬转:稳表达
组成型或诱导性: 组成型
表达水平: 高
蛋白标签:
| 5’测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
|---|---|
| 3’测序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
GenBank号:
其他属性:
Hosts: E.coli NM522, E.coli INValphaF’, mammal. Related vectors: EBV.
质粒图谱:
质粒序列:
LOCUS Exported 10186 bp ds-DNA circular SYN 22-AUG-2016DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10186)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, August 22, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..10186
/organism=”synthetic DNA construct”
/mol_type=”other DNA”
enhancer 4..383
/note=”CMV enhancer”
/note=”human cytomegalovirus immediate early enhancer”
promoter 384..587
/note=”CMV promoter”
/note=”human cytomegalovirus (CMV) immediate early
promoter”
polyA_signal 672..806
/note=”SV40 poly(A) signal”
/note=”SV40 polyadenylation signal”
rep_origin 1529..3318
/note=”oriP”
/note=”Epstein-Barr virus oriP replication origin (Yates et
al., 2000)”
promoter 6066..6170
/gene=”bla”
/note=”AmpR promoter”
CDS 6171..7031
/codon_start=1
/gene=”bla”
/product=”beta-lactamase”
/note=”AmpR”
/note=”confers resistance to ampicillin, carbenicillin, and
related antibiotics”
/translation=”MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW”
rep_origin 7202..7790
/direction=RIGHT
/note=”ori”
/note=”high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication”
promoter 8221..8366
/note=”HSV TK promoter”
/note=”herpes simplex virus thymidine kinase promoter”
CDS 8409..9446
/codon_start=1
/product=”hygromycin B phosphotransferase”
/note=”HygR”
/note=”confers resistance to hygromycin”
/translation=”MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN”
polyA_signal 9488..9536
/note=”HSV TK poly(A) signal”
/note=”herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)”
ORIGIN
1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctcgaggc
661 cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa accacaacta
721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
781 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
841 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
901 ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
1021 tcaggcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga
1081 tcgatgcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
1141 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
1201 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
1261 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
1321 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
1381 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
1441 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
1501 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
1561 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
1621 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
1681 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
1741 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
1801 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
1861 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
1921 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
1981 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
2041 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
2101 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
2161 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
2221 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
2281 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
2341 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
2401 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
2461 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
2521 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
2581 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
2641 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
2701 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
2761 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
2821 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
2881 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
2941 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
3001 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
3061 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
3121 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
3181 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag
3241 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg
3301 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct
3361 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag
3421 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt
3481 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat
3541 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg
3601 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt
3661 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc
3721 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag
3781 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat
3841 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg
3901 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg
3961 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata
4021 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca
4081 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac
4141 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc
4201 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca
4261 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta
4321 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg
4381 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg
4441 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac
4501 ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc
4561 ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
4621 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
4681 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
4741 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
4801 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc
4861 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc
4921 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
4981 ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
5041 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
5101 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc
5161 ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
5221 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg
5281 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac
5341 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc
5401 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac
5461 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc
5521 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat
5581 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca
5641 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc
5701 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc
5761 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg
5821 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga
5881 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat
5941 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
6001 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
6061 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
6121 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
6181 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
6241 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
6301 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
6361 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
6421 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
6481 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
6541 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
6601 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
6661 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
6721 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
6781 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
6841 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
6901 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
6961 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
7021 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
7081 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
7141 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
7201 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
7261 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
7321 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
7381 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
7441 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
7501 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
7561 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
7621 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
7681 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
7741 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
7801 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt
7861 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
7921 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
7981 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
8041 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
8101 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt
8161 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg
8221 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
8281 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
8341 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
8401 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
8461 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
8521 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
8581 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
8641 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
8701 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
8761 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
8821 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
8881 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
8941 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
9001 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
9061 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
9121 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
9181 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
9241 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
9301 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
9361 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
9421 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
9481 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
9541 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
9601 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
9661 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta
9721 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
9781 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
9841 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
9901 cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc
9961 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
10021 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
10081 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
10141 cataatatgt acatttatat tggctcatgt ccaatatgac cgccat
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