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- 库存:
99
- 供应商:
江蓝纯
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥960.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1600.0 |
| 英文名称 | Mouse Histone H2A.X(Phospho-Ser139) Monoclonal Antibody |
|---|---|
| 别名 | Histone H2A.X(Phospho-Ser139) |
| 应用 | WB,IF |
| 稀释比例 | WB1:2000 IHC1:400 |
| 交叉反应 | Human,Mouse |
| 蛋白分子量 | 15KDa |
| Gene ID | 3014 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment. |
| 储存液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 69% Glycerol. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG2a |
| 免疫原 | Synthetic Peptide |
| 性状 | Lyophilized or Liquid |
| Swiss Prot | P16104 |
| 克隆号 | 2A9-B5 |
| 克隆类型 | Monoclonal Antibody |
| 来源 | Mouse |
| 背景资料 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. |
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
Monoclonal Antibody Production Protocol
of debris, rounded and refractive under phase contrast, and that they are harvested in log or late log phase growth (between 3.5 and 9 x105 cells/ml). Thaw cells 7 days before scheduled fusion. Myeloma do not grow well after being in culture
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
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