- ¥520
- 江蓝纯
- RG001020
- 2025年07月15日
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- 库存：
99
- 供应商：
江蓝纯
- 规格：
100ul
| 英文名称 | His Tag Rabbit Polyclonal antibody |
|---|---|
| 浓度 | 1mg/ml |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment. |
| 储存液 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 60% Glycerol |
| 性状 | Lyophilized or Liquid |
| 克隆类型 | Polyclonal antibody |
| 来源 | Rabbit |
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells
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文献和实验Purification and Characterization of His-Tagged Antibody Fragments
, typically His6 , fused to the recombinant protein. In the case of antibody fragments that are composed of different chains, e.g., Fv and Fab, the site of attachment for the His6 tag has to be carefully chosen, and mild chromatographic conditions
Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions
Protein Antigen Expression in Escherichia coli for Antibody Production
Escherichia coli is a frequently used expression system for the generation of protein encoded by genes from diverse kingdoms and, thus, it is well suited for the production of protein antigens for antibody generation. It is a system of choice
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