手机验证
-20℃
6个月
pBAD102 Directional TOPO(pBAD102DirectionalTOPO)
大量
联迈生物
1ug
质粒名称: pBAD102 Directional TOPO
其他名称: pBAD102/D-TOPO
质粒大小(bp): 4471
抗性:氨苄青霉素(Ampicillin)
质粒类型: Bacterial Expression
是否病毒: Nonviral
启动子: araBAD
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: Tightly controlled
蛋白标签: 6x His Tag(C-端),V5 Epitope Tag(C-端);Thioredoxin(N-端),EK 切割位点
5′ 测序引物及序列:TrxFus Forward: 5′-TTCCTCGACGCTAACCTG-3′
3′ 测序引物及序列:pBAD Reverse 5′-GATTTAATCTGTATCAGG-3′
GenBank号:
其他属性: pBAD102/D-TOPO载体是阿拉伯糖调控载体,在无葡萄糖的培养基中,阿拉伯糖正向调控目的基因的表达;
通过调节阿拉伯糖的浓度水平来优化目的蛋白的可溶性表达;
采用TOPO-TA技术,只用5分钟即可将PCR片段连接到载体上去;
pBAD102/D-TOPO载体表达硫氧还蛋白(Thioredoxin)融合蛋白,硫氧还蛋白的存在促进目的蛋白的可溶性,尤其对于溶解性差的蛋白来说,是良好的选择。
质粒图谱:
质粒序列:
1 | AAGAAACCAA | TTGTCCATAT | TGCATCAGAC | ATTGCCGTCA | CTGCGTCTTT | 50 |
51 | TACTGGCTCT | TCTCGCTAAC | CAAACCGGTA | ACCCCGCTTA | TTAAAAGCAT | 100 |
101 | TCTGTAACAA | AGCGGGACCA | AAGCCATGAC | AAAAACGCGT | AACAAAAGTG | 150 |
151 | TCTATAATCA | CGGCAGAAAA | GTCCACATTG | ATTATTTGCA | CGGCGTCACA | 200 |
201 | CTTTGCTATG | CCATAGCATT | TTTATCCATA | AGATTAGCGG | ATCCTACCTG | 250 |
251 | ACGCTTTTTA | TCGCAACTCT | CTACTGTTTC | TCCATACCCG | TTTTTTTGGG | 300 |
301 | CTAGAAATAA | TTTTGTTTAA | CTTTAAGAAG | GAGATATACA | TACCCATGGG | 350 |
351 | ATCTGATAAA | ATTATTCATC | TGACTGATGA | TTCTTTTGAT | ACTGATGTAC | 400 |
401 | TTAAGGCAGA | TGGTGCAATC | CTGGTTGATT | TCTGGGCACA | CTGGTGCGGT | 450 |
451 | CCGTGCAAAA | TGATCGCTCC | GATTCTGGAT | GAAATCGCTG | ACGAATATCA | 500 |
501 | GGGCAAACTG | ACCGTTGCAA | AACTGAACAT | CGATCACAAC | CCGGGCACTG | 550 |
551 | CGCCGAAATA | TGGCATCCGT | GGTATCCCGA | CTCTGCTGCT | GTTCAAAAAC | 600 |
601 | GGTGAAGTGG | CGGCAACCAA | AGTGGGTGCA | CTGTCTAAAG | GTCAGTTGAA | 650 |
651 | AGAGTTCCTC | GACGCTAACC | TGGCCGGCTC | TGGATCCGGT | GATGACGATG | 700 |
701 | ACAAGCTGGG | AATTGATCCC | TTCACCAAGG | GCGAGCTCAA | GCTTGAAGGT | 750 |
751 | AAGCCTATCC | CTAACCCTCT | CCTCGGTCTC | GATTCTACGC | GTACCGGTCA | 800 |
801 | TCATCACCAT | CACCATTGAG | TTTAAACGGT | CTCCAGCTTG | GCTGTTTTGG | 850 |
851 | CGGATGAGAG | AAGATTTTCA | GCCTGATACA | GATTAAATCA | GAACGCAGAA | 900 |
901 | GCGGTCTGAT | AAAACAGAAT | TTGCCTGGCG | GCAGTAGCGC | GGTGGTCCCA | 950 |
951 | CCTGACCCCA | TGCCGAACTC | AGAAGTGAAA | CGCCGTAGCG | CCGATGGTAG | 1000 |
1001 | TGTGGGGTCT | CCCCATGCGA | GAGTAGGGAA | CTGCCAGGCA | TCAAATAAAA | 1050 |
1051 | CGAAAGGCTC | AGTCGAAAGA | CTGGGCCTTT | CGTTTTATCT | GTTGTTTGTC | 1100 |
1101 | GGTGAACGCT | CTCCTGAGTA | GGACAAATCC | GCCGGGAGCG | GATTTGAACG | 1150 |
1151 | TTGCGAAGCA | ACGGCCCGGA | GGGTGGCGGG | CAGGACGCCC | GCCATAAACT | 1200 |
1201 | GCCAGGCATC | AAATTAAGCA | GAAGGCCATC | CTGACGGATG | GCCTTTTTGC | 1250 |
1251 | GTTTCTACAA | ACTCTTTTGT | TTATTTTTCT | AAATACATTC | AAATATGTAT | 1300 |
1301 | CCGCTCATGA | GACAATAACC | CTGATAAATG | CTTCAATAAT | ATTGAAAAAG | 1350 |
1351 | GAAGAGTATG | AGTATTCAAC | ATTTCCGTGT | CGCCCTTATT | CCCTTTTTTG | 1400 |
1401 | CGGCATTTTG | CCTTCCTGTT | TTTGCTCACC | CAGAAACGCT | GGTGAAAGTA | 1450 |
1451 | AAAGATGCTG | AAGATCAGTT | GGGTGCACGA | GTGGGTTACA | TCGAACTGGA | 1500 |
1501 | TCTCAACAGC | GGTAAGATCC | TTGAGAGTTT | TCGCCCCGAA | GAACGTTTTC | 1550 |
1551 | CAATGATGAG | CACTTTTAAA | GTTCTGCTAT | GTGGCGCGGT | ATTATCCCGT | 1600 |
1601 | GTTGACGCCG | GGCAAGAGCA | ACTCGGTCGC | CGCATACACT | ATTCTCAGAA | 1650 |
1651 | TGACTTGGTT | GAGTACTCAC | CAGTCACAGA | AAAGCATCTT | ACGGATGGCA | 1700 |
1701 | TGACAGTAAG | AGAATTATGC | AGTGCTGCCA | TAACCATGAG | TGATAACACT | 1750 |
1751 | GCGGCCAACT | TACTTCTGAC | AACGATCGGA | GGACCGAAGG | AGCTAACCGC | 1800 |
1801 | TTTTTTGCAC | AACATGGGGG | ATCATGTAAC | TCGCCTTGAT | CGTTGGGAAC | 1850 |
1851 | CGGAGCTGAA | TGAAGCCATA | CCAAACGACG | AGCGTGACAC | CACGATGCCT | 1900 |
1901 | GTAGCAATGG | CAACAACGTT | GCGCAAACTA | TTAACTGGCG | AACTACTTAC | 1950 |
1951 | TCTAGCTTCC | CGGCAACAAT | TAATAGACTG | GATGGAGGCG | GATAAAGTTG | 2000 |
2001 | CAGGACCACT | TCTGCGCTCG | GCCCTTCCGG | CTGGCTGGTT | TATTGCTGAT | 2050 |
2051 | AAATCTGGAG | CCGGTGAGCG | TGGGTCTCGC | GGTATCATTG | CAGCACTGGG | 2100 |
2101 | GCCAGATGGT | AAGCCCTCCC | GTATCGTAGT | TATCTACACG | ACGGGGAGTC | 2150 |
2151 | AGGCAACTAT | GGATGAACGA | AATAGACAGA | TCGCTGAGAT | AGGTGCCTCA | 2200 |
2201 | CTGATTAAGC | ATTGGTAACT | GTCAGACCAA | GTTTACTCAT | ATATACTTTA | 2250 |
2251 | GATTGATTTA | AAACTTCATT | TTTAATTTAA | AAGGATCTAG | GTGAAGATCC | 2300 |
2301 | TTTTTGATAA | TCTCATGACC | AAAATCCCTT | AACGTGAGTT | TTCGTTCCAC | 2350 |
2351 | TGAGCGTCAG | ACCCCGTAGA | AAAGATCAAA | GGATCTTCTT | GAGATCCTTT | 2400 |
2401 | TTTTCTGCGC | GTAATCTGCT | GCTTGCAAAC | AAAAAAACCA | CCGCTACCAG | 2450 |
2451 | CGGTGGTTTG | TTTGCCGGAT | CAAGAGCTAC | CAACTCTTTT | TCCGAAGGTA | 2500 |
2501 | ACTGGCTTCA | GCAGAGCGCA | GATACCAAAT | ACTGTCCTTC | TAGTGTAGCC | 2550 |
2551 | GTAGTTAGGC | CACCACTTCA | AGAACTCTGT | AGCACCGCCT | ACATACCTCG | 2600 |
2601 | CTCTGCTAAT | CCTGTTACCA | GTGGCTGCTG | CCAGTGGCGA | TAAGTCGTGT | 2650 |
2651 | CTTACCGGGT | TGGACTCAAG | ACGATAGTTA | CCGGATAAGG | CGCAGCGGTC | 2700 |
2701 | GGGCTGAACG | GGGGGTTCGT | GCACACAGCC | CAGCTTGGAG | CGAACGACCT | 2750 |
2751 | ACACCGAACT | GAGATACCTA | CAGCGTGAGC | TATGAGAAAG | CGCCACGCTT | 2800 |
2801 | CCCGAAGGGA | GAAAGGCGGA | CAGGTATCCG | GTAAGCGGCA | GGGTCGGAAC | 2850 |
2851 | AGGAGAGCGC | ACGAGGGAGC | TTCCAGGGGG | AAACGCCTGG | TATCTTTATA | 2900 |
2901 | GTCCTGTCGG | GTTTCGCCAC | CTCTGACTTG | AGCGTCGATT | TTTGTGATGC | 2950 |
2951 | TCGTCAGGGG | GGCGGAGCCT | ATGGAAAAAC | GCCAGCAACG | CGGCCTTTTT | 3000 |
3001 | ACGGTTCCTG | GCCTTTTGCT | GGCCTTTTGC | TCACATGTTC | TTTCCTGCGT | 3050 |
3051 | TATCCCCTGA | TTCTGTGGAT | AACCGTATTA | CCGCCTTTGA | GTGAGCTGAT | 3100 |
3101 | ACCGCTCGCC | GCAGCCGAAC | GACCGAGCGC | AGCGAGTCAG | TGAGCGAGGA | 3150 |
3151 | AGCGGAAGAG | CGCCTGATGC | GGTATTTTCT | CCTTACGCAT | CTGTGCGGTA | 3200 |
3201 | TTTCACACCG | CATATGGTGC | ACTCTCAGTA | CAATCTGCTC | TGATGCCGCA | 3250 |
3251 | TAGTTAAGCC | AGTATACACT | CCGCTATCGC | TACGTGACTG | GGTCATGGCT | 3300 |
3301 | GCGCCCCGAC | ACCCGCCAAC | ACCCGCTGAC | GCGCCCTGAC | GGGCTTGTCT | 3350 |
3351 | GCTCCCGGCA | TCCGCTTACA | GACAAGCTGT | GACCGTCTCC | GGGAGCTGCA | 3400 |
3401 | TGTGTCAGAG | GTTTTCACCG | TCATCACCGA | AACGCGCGAG | GCAGCAGATC | 3450 |
3451 | AATTCGCGCG | CGAAGGCGAA | GCGGCATGCA | TAATGTGCCT | GTCAAATGGA | 3500 |
3501 | CGAAGCAGGG | ATTCTGCAAA | CCCTATGCTA | CTCCGTCAAG | CCGTCAATTG | 3550 |
3551 | TCTGATTCGT | TACCAATTAT | GACAACTTGA | CGGCTACATC | ATTCACTTTT | 3600 |
3601 | TCTTCACAAC | CGGCACGGAA | CTCGCTCGGG | CTGGCCCCGG | TGCATTTTTT | 3650 |
3651 | AAATACCCGC | GAGAAATAGA | GTTGATCGTC | AAAACCAACA | TTGCGACCGA | 3700 |
3701 | CGGTGGCGAT | AGGCATCCGG | GTGGTGCTCA | AAAGCAGCTT | CGCCTGGCTG | 3750 |
3751 | ATACGTTGGT | CCTCGCGCCA | GCTTAAGACG | CTAATCCCTA | ACTGCTGGCG | 3800 |
3801 | GAAAAGATGT | GACAGACGCG | ACGGCGACAA | GCAAACATGC | TGTGCGACGC | 3850 |
3851 | TGGCGATATC | AAAATTGCTG | TCTGCCAGGT | GATCGCTGAT | GTACTGACAA | 3900 |
3901 | GCCTCGCGTA | CCCGATTATC | CATCGGTGGA | TGGAGCGACT | CGTTAATCGC | 3950 |
3951 | TTCCATGCGC | CGCAGTAACA | ATTGCTCAAG | CAGATTTATC | GCCAGCAGCT | 4000 |
4001 | CCGAATAGCG | CCCTTCCCCT | TGCCCGGCGT | TAATGATTTG | CCCAAACAGG | 4050 |
4051 | TCGCTGAAAT | GCGGCTGGTG | CGCTTCATCC | GGGCGAAAGA | ACCCCGTATT | 4100 |
4101 | GGCAAATATT | GACGGCCAGT | TAAGCCATTC | ATGCCAGTAG | GCGCGCGGAC | 4150 |
4151 | GAAAGTAAAC | CCACTGGTGA | TACCATTCGC | GAGCCTCCGG | ATGACGACCG | 4200 |
4201 | TAGTGATGAA | TCTCTCCTGG | CGGGAACAGC | AAAATATCAC | CCGGTCGGCA | 4250 |
4251 | AACAAATTCT | CGTCCCTGAT | TTTTCACCAC | CCCCTGACCG | CGAATGGTGA | 4300 |
4301 | GATTGAGAAT | ATAACCTTTC | ATTCCCAGCG | GTCGGTCGAT | AAAAAAATCG | 4350 |
4351 | AGATAACCGT | TGGCCTCAAT | CGGCGTTAAA | CCCGCCACCA | GATGGGCATT | 4400 |
4401 | AAACGAGTAT | CCCGGCAGCA | GGGGATCATT | TTGCGCTTCA | GCCATACTTT | 4450 |
4451 | TCATACTCCC | GCCATTCAGA | G | 4471 |
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