- ¥560 - 11760
- 江蓝纯
- P00114
- 2025年07月12日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
江蓝纯
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ¥560.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ¥1170.0 |
| 规格： | 500ug | 产品价格： | ¥7280.0 |
| 规格： | 1mg | 产品价格： | ¥11760.0 |
| Known as | Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB |
|---|---|
| Derived from | E.coli |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris,200mM NaCl,pH8.0. |
| Shipping | The product is shipped at ambient temperature. |
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months |
| Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxin | Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Background | NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. |
Recombinant Mouse beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Met130-Arg239 is expressed.
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文献和实验Euthanasia Euthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress
0.5 ml of 5 mg/ml insulin per 500 ml bottle of EMEM (final conc’n = 5μg/ml). Epidermal Growth Factor (EGF) Recombinant human EGF; GibcoBRL, cat # 13247-051 Prepare a 10μg/ml stock: Dissolve 100μg EGF in 1 ml 0.1% BSA (10 mg ultrapure BSA
Culture of Mouse Olfactory Sensory Neurons
. Mouse breeding and colony management. Curr. Protoc. Mouse Biol. 1:239‐264. Chen, H., Dadsetan, S., Fomina, A.F., and Gong, Q. 2008. Expressing exogenous functional odorant
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