- $569
- Alomone
- 以色列
- LK-3100
- 2025年07月13日
- 见官方网站
- 见说明书
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
液体或冻干粉
- 保存条件：
-20°C
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
6个月
- 抗原来源：
见说明书
- 目录编号：
LK-3100
- 级别：
高
- 库存：
大量
- 供应商：
上海信裕生物科技有限公司
- 宿主：
见说明书
- 应用范围：
见官方网站
- 浓度：
见官方网站
- 靶点：
见官方网站
- 抗体英文名：
ASIC3 Channel Antibody and Membrane Fractions Kit
- 抗体名：
ASIC3 Channel Antibody and Membrane Fractions Kit
- 规格：
4 Vials
ASIC3 Channel Antibody and Membrane Fractions Kit
Kit Contains Anti-ASIC3 Antibody and all Controls Necessary for Robust Western Blot AnalysisCat #: LK-3100
4 Vials
ASIC3 Channel Overexpressed Membrane Fractions (#LX-100) are Xenopus oocyte membrane fractions overexpressing ASIC3 channel. ASIC3 Channel Overexpressed Membrane Fractions are your positive control for validating Alomone Labs ASIC3 channel Anti-ASIC3 antibody (#ASC-018).
Overexpressed Membrane Fractions are:
✓ Lyophilized powder
✓ Economical
✓ Shipped at room temperature (no need for dry ice and extra shipping costs)
✓ User-friendly & time-saving. Just add water, sample buffer and load your gel
- Compounds
- Scientific Background
- Related Products
| Product Name | Cat # | Size |
|---|---|---|
Anti-ASIC3 |
ASC-018 | 1 x 0.2 ml |
ASIC3 Channel Overexpressed Membrane Fractions |
LX-100 | 2 x 0.1 ml |
Note
Included with the products in this kit:
1 x 40 µg Anti-ASIC3 control peptide antigen
1 x 0.1 ml lyophilized non-injected Xenopus oocyte overexpressed membrane fraction
Scientific Background
ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes another three members ASIC1, ASIC2 and ASIC4. The ASICs are in fact part of a larger superfamily termed degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini.
The functional channel is composed of 4 subunits that can be assembled as homo or heterotetramers with the other ASIC subunits.
A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.
Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.
Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.
References
- Kellenberger, S. and Schild, L. (2002) Physiol. Rev. 82, 735.
- Chen, C.C. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 8992.
- Price, M.P. et al. (2001) Neuron. 32, 1071.
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