Anti-ASIC1

Anti-ASIC1

Amiloride-sensitive brain sodium channel, BnaC2, ACCN2
Cat #: ASC-014
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IC, IH, IP, WB
May also work in: IFC
Reactivity: H, M, R

Anti-ASIC1Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Anti-ASIC1 (#ASC-014) is an antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC1 from rat, human, and mouse samples, and recognizes both ASIC1 isoforms.

For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.

• Applications
• Specifications
• Scientific Background
• Citations
• Related Products

Western blot

Western blot analysis of rat brain membranes:
1. Anti-ASIC1 antibody (#ASC-014), (1:200).   
2. Anti-ASIC1 antibody, preincubated with the control peptide antigen.
Immunoprecipitation
CHO-K1 and D54-MG transfected cells (see Kapoor, N. et al. (2012) J. Biol. Chem. 284, 24526.).
Immunohistochemistry

Expression of ASIC1 in rat brain
Immunohistochemical staining of rat globus pallidus using Anti-ASIC1 antibody (#ASC-014). A. Parvalbumin (PV) positive neurons are shown (green). B. Neurons with typically enmeshed dendritic trees stain intensely for ASIC1 (red). C. Double labeling with mouse anti-Parvalbumin (green) reveals strong co-localization of PV with ASIC1.
Immunocytochemistry
Mouse bone marrow derived macrophage (BMMs) (see Kong, X. et al. (2013) Cell. Immunol. 281, 44.

Human SH-SY5Y (see Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.
Immunogen
Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession number P55926). Intracellular, C-terminus.

HomologyHuman - identical; mouse - 19/20 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
SpecificityRecognizes isoforms ASIC1α and ASIC1β.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 μg peptide per 1 μg antibody.
Scientific background

ASIC1 is a member of a family of Na⁺ channels that are activated by external protons. The family includes another three members ASIC2, ASIC3 and ASIC4. 

The ASICs are in fact part of a larger superfamily termed epithelial Na⁺ channels (EnaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini.  

There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions.  

The ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium.  

Lately, ASIC1 has been implicated in processes such as learning and memory in the central nervous system.

References

1. Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735.
2. Wemmie, J. A. et al. (2002) Neuron 34, 463.
3. Wemmie, J. A. et al. (2003) J. Neurosci. 23, 5496.