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- 详细信息
- 文献和实验
- 技术资料
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详见说明书
- 保质期:
详见说明书
- 英文名:
Tris(dibenzylideneacetone)dipalladium-chloroform adduct
- 库存:
2~15
- 供应商:
上海禾午生物科技有限公司
- CAS号:
52522-40-4
- 规格:
0.98
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文献和实验Deproteination using phenol/chloroform
to a final concentration of 0.1%, and equilibrated three times with an equal volume of 1M Tris.HCl, pH 7.0. The final Tris wash is replaced with TE (10mM Tris, 1mM EDTA, pH8.0) and the phenol stored in the dark at 4℃. 8-hydroxyquinoline is added
Deproteination using phenol/chloroform
mM Tris, 1mM EDTA, pH8.0) and the phenol stored in the dark at 4℃. 8-hydroxyquinoline is added to prevent oxidation of the phenol and to act as an inhibitor of RNases. 'Chloroform' as used is a water saturated 24:1(v/v) mixture of chloroform and isoamyl
DNA Isolation from Rhizobium by Phenol Chloroform Method
l TE. Add 40 μl 10% SDS and 5μl Proteinase K (20mg/ml). Incubate at 56 o C for 45 mins. Add 400 μl of tris saturated phenol (pH 8.0). Centrifuge at 10,000 rpm for 10 mins. Take the supernatant and add 200 μl of tris
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