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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
dNTPs Mix(10mM
- 库存:
99
- 供应商:
江蓝纯
- 规格:
500ul/1ml/10ml/10*1ml/100ml
| 规格: | 500ul | 产品价格: | ¥68.0 |
|---|---|---|---|
| 规格: | 1ml | 产品价格: | ¥116.0 |
| 规格: | 10ml | 产品价格: | ¥800.0 |
| 规格: | 10*1ml | 产品价格: | ¥1000.0 |
| 规格: | 100ml | 产品价格: | ¥6400.0 |
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文献和实验SequalPrep Long PCR Kit with dNTPs
mix as follows. Amounts below are for a 20-μl reaction. Scale the reaction volume and multiply by number of reactions as needed. Table 1 Component
. Lysis buffer (store on the benchtop without proteinase K). 60 g/ml proteinase K 10mM Tris-Cl, pH 8.2 50mM KCl 2.5mM MgCl2 0.45% Tween-20 0.05% gelatin Master mix . Prepare a master mix containing the following
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
for 10min Immediately neutralize the mix with 5ml of 1M Tris-HCl, pH= 7.5 ・ If only using single label, add 38.5ml of 10mM Tris-HCl, pH=8.0, 1mM EDTA (Volume = 67ml) ・ For dual labels, combine the Cy3 with the Cy5 (Volume = 57ul). Then rinse Cy
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