Anti-Aquaporin 9
AQP9
Cat #: AQP-009
Sizes: 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
Reactivity: M, R
Anti-Aquaporin 9Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
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Anti-Aquaporin 9 (#AQP-009) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP9 from mouse and rat samples.
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- Applications
- Specifications
- Scientific Background
- Citations
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Western blot
Western blot analysis of rat brain (lanes 1 and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes:
1,2,3. Anti-Aquaporin 9 antibody (#AQP-009), (1:200).
4,5,6. Anti-Aquaporin 9 antibody, preincubated with the control peptide antigen.
Western blot analysis of rat H4-II-E-C3 hepatoma cell line lysate:
1. Anti-Aquaporin 9 antibody (#AQP-009), (1:200).
2. Anti-Aquaporin 9 antibody, preincubated with the control peptide antigen.
Western blot analysis of mouse brain membranes:
1. Anti-Aquaporin 9 antibody (#AQP-009), (1:200).
2. Anti-Aquaporin 9 antibody, preincubated with the control peptide antigen.
Immunohistochemistry
Expression of Aquaporin 9 in rat liver
Immunohistochemical staining of rat liver paraffin embedded sections using Anti-Aquaporin 9 antibody (#AQP-009) (1:100). Aquaporin 9 (brown staining) is expressed in hepatocytes of the liver parenchyma.
Hematoxilin is used as the counterstain.
Expression of Aquaporin 9 in rat epididymus
Immunohistochemical staining of rat testis paraffin embedded section using Anti-Aquaporin 9 antibody (#AQP-009), (1:100). Aquaporin 9 (brown staining) is expressed in the columnar epithelium of the epididymus (arrows). Hematoxilin is used as the counterstain.
Immunogen
Peptide (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP-9 (Accession number P56627). Intracellular, N-terminus.
HomologyMouse - 13/16 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 0.025% NaN3.
SpecificityUnlikely to recognize human samples.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 μg peptide per 1 μg antibody.
Scientific background
Aquaporin 9 (AQP-9) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include AQP-1, AQP-2, AQP4, AQP-5, AQP-6 and AQP-8, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.2
AQP-9 is expressed in several tissues including brain, liver, testis and spleen.
In the liver AQP-9 has a central role in glycerol metabolism as demonstrated in experiments using AQP-9 knockout mice: mice deficient in AQP-9 expression showed enhanced plasma levels of glycerol and triglycerides.3
References
- Rojek, A.M. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 3609.
- King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
- Koyama, Y. et al. (1997) J Biol Chem 272, 30329.